ruby pelingon

Uncovering bacterial pseudaminylation with pan-specific antibody tools - Nature Chemical Biology Pseudaminic acids (Pse) are a family of carbohydrates found within bacterial lipopolysaccharides, capsular polysaccharides and glycoproteins. Now, monoclonal antibodies have been developed that recogn...

Monoclonal antibodies against pseudaminic acid (Pse) – present in bacterial LPS, capsular polysaccharides, and glycoproteins – have been developed that enable Pse glycoproteome profiling and show therapeutic potential #glycotime

www.nature.com/articles/s41...

Exciting new #glycotime work from the Woo lab @harvard.edu 👏

#glycotime!

Iterative Bump-and-hole engineering creates a bioorthogonal reporter for N-acetylglucosaminyltransferase I https://www.biorxiv.org/content/10.64898/2026.01.16.699845v1

A biocompatible Lossen rearrangement in Escherichia coli - Nature Chemistry Biocompatible chemistry merges chemo-catalytic reactions with cellular metabolism for sustainable small-molecule synthesis. Now a biocompatible Lossen rearrangement has been demonstrated to control ba...

www.nature.com/articles/s41...

New cell-based glycosyltransferase activity assay using mammalian surface display with click chemistry enables rapid quantification of GT activity. Screening of 1680 pig ST3Gal1 mutants yielded enzymes with improved enzymatic properties.
tinyurl.com/5e5t4dmv
#glycotime

Uncovering mRNA sequences that control translation initiation Nature Reviews Molecular Cell Biology, Published online: 21 May 2025; doi:10.1038/s41580-025-00862-zIn this Tools of the Trade article, Abdallah (Gilbert lab) describes the development of direct analysis of ribosome targeting (DART), a tool designed to explore 5' UTR sequences for their potential to efficiently initiate translation.

ICYMI: New Online! Uncovering mRNA sequences that control translation initiation

Chemical proteomics for a comprehensive understanding of functional activity and the interactome Traditional mass spectrometry (MS)-based proteomics aims to detect and measure protein expression on a global scale and elucidate the link between protein function and phenotypic characteristics. Although advances in MS technology have significantly broadened the scope of detectable proteomes, these methodologies p

Don't miss this #OpenAccess Tutorial review by Kostiantyn Kozoriz and Jun-Seok Lee

"Chemical proteomics for a comprehensive understanding of functional activity and the interactome"

Read the full Tutorial review here 👇
pubs.rsc.org/en/cont...

New Review out!
Can we eventually design not only proteins, but entire cells from scratch? Check out the review I wrote in @natrevbioeng.nature.com about how to combine protein design with synthetic cell science.
Link: www.nature.com/articles/s44...

Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins - Nature Biotechnology Biomacromolecules are delivered into cells using nanoparticles made of elastin and endosomal escape sequences.

Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins - @bidmcreseach.bsky.social go.nature.com/4mxazJw

Protein catalysts designed to do non-natural chemistry Researchers couple AI and chemical know-how to create tools for cyclopropanation and silylation reactions

Researchers have combined artificial intelligence (AI) and their own chemical know-how to create protein catalysts that can perform chemical reactions not found in nature. cen.acs.org/synthesis/bi... 🧪 #biosky #chemsky

Specificity in glycosylation! Have you ever wondered why some glycosyltransferases recognize glycoproteins, some glycolipids, and some both? Together with @kmikolajczyk.bsky.social and @rkaczmarekdr.bsky.social, we try to answer that complicated question.

#glycotime
tinyurl.com/5b6kxhp3

Biosynthesis of nitrile-containing amino acids for rapid protein conjugation and fluorogenic labeling The biosynthesis of bioorthogonal chemical handles for precise protein labeling remains a significant challenge. In this issue of Chem, Thomas Huber and colleagues report the successful biosynthesis of nitrile-containing amino acids and their site-specific incorporation into proteins, enabling efficient protein conjugation and fluorogenic labeling.

Online now: Biosynthesis of nitrile-containing amino acids for rapid protein conjugation and fluorogenic labeling

Edible mRNA vaccine in lettuce chloroplasts - Nature Reviews Bioengineering Messenger RNA (mRNA) vaccines hold promise for creating new and improved immunizations; however, their delivery is challenging in terms of compliance, reactogenicity, cost and scalability. In response...

www.nature.com/articles/s44...

Where do proteins go in cells? Next-generation methods map the molecules’ hidden lives Spatial proteomics is helping biologists to uncover how cells work by mapping where proteins operate.

www.nature.com/articles/d41...

RNA-binding proteins and glycoRNAs form domains on the cell surface for cell-penetrating peptide entry Mammalian cells present RNA-binding proteins on the cell surface that form clustered domains containing glycoRNAs.

www.cell.com/cell/fulltex...

A subcellular map of translational machinery composition and regulation at the single-molecule level Millions of ribosomes are packed within mammalian cells, yet we lack tools to visualize them in toto and characterize their subcellular composition. In this study, we present ribosome expansion micros...

Exciting new work from Barna Lab @stanfordmedicine.bsky.social

www.science.org/doi/10.1126/...

A new paper led by @mlagisz.bsky.social - how to know how good your search string is

Branched chemically modified poly(A) tails enhance the translation capacity of mRNA - Nature Biotechnology mRNA with engineered poly(A) tails produces prolonged higher levels of protein.

www.nature.com/articles/s41...

Carbohydrate-active enzymes from Akkermansia muciniphila break down mucin O-glycans to completion - Nature Microbiology Biochemical characterization of 66 carbohydrate-active enzymes from the gut microorganism Akkermansia muciniphila reveals that these enzymes can break down a range of host glycans, including mucin, which they degrade to completion.

Biochemical characterization of 66 carbohydrate-active enzymes from the gut microorganism Akkermansia muciniphila reveals that these enzymes can break down a range of host glycans, including mucin, which they degrade to completion, according to a Nature Microbiology paper. 🧪

Protein codes promote selective subcellular compartmentalization Cells have evolved mechanisms to distribute ~10 billion protein molecules to subcellular compartments where diverse proteins involved in shared functions must assemble. Here, we demonstrate that prote...

www.science.org/doi/10.1126/...