Adrià Sogues

Great science, nice environment and a very exciting project!! Highly recommended! 👇

SAVE THE DATE!
The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027.
Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social.
More info soon.

#MicroSky

#microsky

Fun fact: the 2D class averages of recombinant AbpX super-bundles look uncannily like a French pastry called carré confiture. How sweet?!

Preprint out! That was a really enjoyable collaboration! We report the structure and higher-order assembly of AbpX, a biofilm component in Archaea. And as a bonus, this protein gave me one of the most beautiful crystals I’ve ever had! 🤩💎

AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structure determination - Nature Methods An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of pred...

www.nature.com/articles/s41...

Apply now to attend the next EMBO Bacterial Networks meeting #EMBOBacNet

🗓️13-18 September 2026
📍Sant Feliu de Guixols, Spain

📝Program and registration info: meetings.embo.org/event/26-bac...

👩‍🔬Organised with co-chair @s-lab.bsky.social and ECR @coralietesseur.bsky.social

#MicroSky

S-layer, a (re)blooming field and a fascinating world for both microbiologists and structural biologists. A very complete and up-to-date review of S-layer functions, structure, and biogenesis. Congrats to all the authors!

🦠🔬🤖🧑‍💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training

📜 www.biorxiv.org/content/10.1...

The line-up I would dream of 🤩

Cryo-Flow : when science freezes and flows ❄️ YouTube video by Institut Pasteur EDUCATION

Cryo-Flow : when science freezes and flows ❄️

After turning her thesis on tuberculosis into a rap, Yaëlle Wormser aka Lady Pipette, doctoral student at the Institut Pasteur, is back with a new track — Cryo-Flow — a song that turns Cryo-Electron Microscopy (Cryo-EM) into rhythm & rhyme.

@pasteur.fr

Thanks Yoann! I always hoped to come back, even though I had an amazing time in Brussels! I’ll definitely miss it!

CRISPRi on #Bdellovibrio to study essential genes 🤗🤩 #MicroSky

A new chapter begins! Happy to be back at the @pasteur.fr , a place that feels like home. Excited for the journey ahead and all the science to come!

Pasteur-Paris University International Doctoral Programme, The call for enrollment of students in October 2026 will be open from September 1st to October 20th, 2025

🎓 Applications for Institut Pasteur's PhD programs open Sept 1-Oct 20, 2025 for October 2026 entry.

Apply to up to 4 projects across 6 programs: PPU, PPU-IMAGINE @institutimagine.bsky.social, PPU-@ox.ac.uk, PPU-EID @upcite.bsky.social & more 🧬

Register now ✍️ www.pasteur.fr/en/education...

Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes - Nature Communications B. thuringiensis spores contain uncharacterized protein filaments that extend from the surface of the exosporium. Here, the authors show that these filaments feature conserved β-barrel neck domains an...

Many spores from the Firmicutes group are decorated with hair-like structures. Since the 60s, they remained a mystery. @remaut-lab.bsky.social named these fibres ENdospore Appendages (ENAs). Using Cryo-ID, we report the identity, structure and function of F-ENA.
www.nature.com/articles/s41...

Thrilled to see our work published in @embojournal.org
#asgard #archaea #cytoskeleton #tubulin #FtsZ
We sincerely appreciate the constructive peer review and the reviewers’ thoughtful, supportive feedback, which greatly strengthened our work.

Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria Protein–protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein net...

Excited to share our newly book chapter on using #miniTurbo proximity labeling in #bacteria
We wrote it as a hands-on guide for anyone looking to get started with this approach.
Congrats to @coralietesseur.bsky.social and Ophélie Remy 👏 @lalouxlab.bsky.social
link.springer.com/protocol/10....

Mapping the ultrastructural topology of the corynebacterial cell surface The unique cell envelope of corynebacteria remains poorly understood at the ultrastructural level. This study maps the C. glutamicum cell surface, revealing a patchy S-layer organization and the speci...

Don’t forget to check out the fantastic complementary work by @tbharat-lab.bsky.social and @bupbuse.bsky.social

journals.plos.org/plosbiology/...

Huge thanks to all the co-authors: @mikesleutel.bsky.social and @remaut-lab.bsky.social (from VIB-VUB), Julienne Petit and Anne Marie Wehenkel (from @pasteur.fr @ipdbsc.bsky.social), Nicolas Bayan(from @i2bcparissaclay.bsky.social ) and Daniela Megrian (from @ipmontevideo.bsky.social)

4/4🧵Finally, we believe that our engineered PS2 S-layer can be exploited in the biomaterials and cell display fields, as S-layers are regular self-assembling platforms in which we can now bind other proteins covalently. We've also added our plasmids to @addgene.bsky.social

3/4🧵How does the S-layer grow during cell division? Using pulse-chase labeling with the SpyTag-SpyCatcher system, we discovered that new S-layer is inserted exclusively at the cell poles, exactly where peptidoglycan synthesis occurs. S-layer assembly and growth are tightly coordinated!

2/4🧵We show across different strains that the S-layer provides mechanical support to the cell wall, especially when it's compromised (such as after lysozyme treatment). The S-layer acts as a protective scaffold, reinforcing the envelope under stress.

1/4🧵 We show across different strains that the S-layer provides mechanical support to the cell wall, especially when it's compromised (such as after lysozyme treatment). The S-layer acts as a protective scaffold, reinforcing the envelope under stress.

Cryo-EM structure and polar assembly of the PS2 S-layer of Corynebacterium glutamicum | PNAS The polar-growing Corynebacteriales have a complex cell envelope architecture characterized by the presence of a specialized outer membrane compose...

Happy to see the final version out! Cryo-EM structure of the PS2 S-layer in Corynebacterium 🔬! We show it provides mechanical support to the cell wall and assembles at the poles in coordination with PG. It can also be used for covalent surface display.

www.pnas.org/doi/10.1073/...

#microsky

Our review on contact-dependent #predation is now out ! We define predation processes and describe how predators kill and eat their prey 😈 Repost appreciated ❤️

Bacterial cell wall & periplasm paradigm shift - important read #MicroSky

www.nature.com/articles/s41...

Strategies and mechanisms of contact-dependent predation in bacteria Predatory bacteria kill other bacteria to acquire nutrients. Among diverse strategies to kill and feed on prey, contact-dependent predation stands out…

#MicroSky Summer read!

Predatory bacteria kill and eat other bacteria. Their deadly actions come in many flavors, often via direct physical contact between predator &
prey 🧛

How do they do it?
Our review 📖 @coralietesseur.bsky.social @ysantin.bsky.social

www.sciencedirect.com/science/arti...

New review from our lab! Compartmentalization during bacterial spore formation. Focusing on latest discoveries in Bacillus subtilis #subtiwiki

www.sciencedirect.com/science/arti...

Folddisco finds similar (dis)continuous 3D motifs in large protein structure databases. Its efficient index enables fast uncharacterized active site annotation, protein conformational state analysis and PPI interface comparison. 1/9🧶🧬
📄 www.biorxiv.org/content/10.1...
🌐 search.foldseek.com/folddisco