We have started a project trying to predic the interactions/structures of all yeast protein pairs using an AlphaFold pooling approach. We are making the current dataset open and we welcome collaborations.
www.evocellnet.com/2026/03/mapp...
Mammalian cells form hibernating disomes akin to those in bacterial cells - but connected via ribosomal RNA
www.science.org/doi/10.1126/...
co-first with: @andschwarz.bsky.social
with: @lea-dietrich.bsky.social, @sgiando.bsky.social, @erin-schuman.bsky.social and many more
𧡠0/6
#MolBio π§ͺ
Please share!
My group at @zmbp-tuebingen.bsky.social is offering a post-doctoral position (4 years). We look for a structural biologist with experience in Cryo-EM/Cryo-ET to investigate the mechanisms of host invasion by pathogenic fungi. Deadline February 28th!
uni-tuebingen.de/universitaet...
LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes β revealing a new paradigm for lysosomal failure.
π doi.org/10.64898/202...
#CryoET
New preprint from the lab! π
We combined in situ correlative cryo-ET π₯½π¬with multiscale simulations π₯οΈβ¨οΈ to study condensates inside yeast cells π₯Όπ§ͺ
Link below π
Prohibitins arenβt hanging out just anywhere in the membrane β they prefer the πββοΈπβ¨VIP sectionπ
πͺ©π₯
Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.
s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. WeΒ analyzeΒ thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
Now that itβs November and Halloween is over, is it okay to put up the Christmas tree yet?
Orange and green poster of the In-situ structural biology by Cryo-FIB and Cryo-ET in Frankfurt am Main, with EMBO logo. The poster promotes childcare grants, travel grants and that this is a sustainable event.
Have you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? π§ Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts!
π Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
π Frankfurt | π April 12β20, 2026
Figure 1 from the preprint
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And thereβs a surprise at the end π΅οΈ
www.biorxiv.org/content/10.1...
#teamtomo
Text box says: "We are hiring".
Are you ready to lead ground-breaking research in Infection Biochemistry?
We are recruiting outstanding scientists to establish research groups based at @cssbhamburg.bsky.social tackling fundamental questions in molecular mechanisms of infection.
embl.wd103.myworkdayjobs.com/en-US/EMBL/j...
3D-Animation of the nanobody (light yellow/semi-transparent) inhibiting HSV-2 glycoprotein B. The different domains of gB are color-coded. Three domains are held together by the footprint of the nanobody. IMAGE: Benjamin Vollmer / Centre for Structural Systems Biology
Researchers from CSSB & @mpi-nat.bsky.social have generated a highly potent nanobody that neutralizes a protein essential for the herpes virus infection. Published in @nature.com, the findings promise new therapies to treat & prevent infections @leibnizliv.bsky.social @uni-hamburg.de
t1p.de/cxt7a
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
More great cryo-ET benchmarking work from my colleagues Maarten Tuijtel, TomΓ‘Ε‘ Majtner, Beata TuroΕovΓ‘, & @becklab.bsky.social: Systematic study of how tilt increment choice affects downstream TM & STA. Preprint has the optimally balanced tilt increment. #TeamTomo www.biorxiv.org/content/10.1...
Weβre hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! π§¬βοΈ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. π Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...
New luminescent animal unlocked!
Proud to share our first lab pre-print: βSND3 is the membrane insertase within a fungal multipass transloconβ where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion β‘οΈ doi.org/10.1101/2025...
Speak up to prevent a major loss for the field. CASP, which helped shaping structural biology including AlphaFold is on the verge of shutting down. NIH funding has lapsed, UC Davis support ends and the core team is being let go. Painfully shortsighted.
This is Anaβs third ERC grant! Weβre proud to celebrate her success and groundbreaking work here at the Max Planck Institute of Biophysics. #ERCAdG Read more here π www.biophys.mpg.de/2872321/garcia-saez-awarded-erc-grant
Our latest research is out π !
This study provides an interaction map of Sla2 & the regulatory proteins Sla1 & Pan1, predicted by AI modeling & confirmed by molecular biophysics.
@lucasdefelipe.bsky.social @meytallandau.bsky.social
@emilgustavsson.bsky.social
@embl.org
@cssbhamburg.bsky.social
The electron cryo-microscope adapted for use at liquid nitrogen temperatures within its custom-made black box.
A colder frontier in cryo-EM π§ͺπ¬
Chris Russoβs group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Excited to share our preprint on the molecular architecture of heterochromatin in human cells π§¬π¬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, TuroΕovΓ‘ lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
π Preprint here tinyurl.com/3a74uanv
Look at those nucleosomes! 𧬠Impressive work on chromatin architecture from our colleagues, check it out below! β¬οΈ
Swinging lever mechanism of myosin shown by time-resolved cryoEM www.nature.com/articles/s41... so glad to finally be able to share this with the community! @astburycentre.bsky.social @astbury-bsl.bsky.social @stemuench.bsky.social @snmcmillan.bsky.social @davidklebl.bsky.social
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
Hey #teamtomo, if you often find yourself using the excellent membrain-seg from @lorenzlamm.bsky.social et al., you might find my napari plugin "napari-segselect" useful. Let's say your tomogram contains the edges of two bacterial cells, each with a membrane and cell wall:
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
Interesting sample preparation method moving away from blotting
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... Whatβs new?
I will think of this paper next time I am there... π
