A short Bluetorial on plots of NIH application success rates as a function of percentile.
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09.03.2026 14:56
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A short Bluetorial on plots of NIH application success rates as a function of percentile.
1/20
I don’t know. Impossible to reach. No one replying to inquiry email.
Does anyone know of an anti mStayGold antibody that works for immunoblots / IP? Anyone tried the one from HUABIO (not even sure that’s a real company)? Thanks!
So, is GSK3 locally activated / inactivated in developing neurons? Where and how?
Just in time for 2026!
Hmmm … nothing 😁
The hidden danger of Biorender
(& the death of scientific illustration)
A short thread 🧵
🧪hey all, a friend and colleague, @maddoxfornc.bsky.social, is running for Congress in North Carolina. He is a scientist, professor, and parent, perspectives that are especially important right now. I hope you will support his run for Congress. Find out more here: www.maddoxforcongress.com/about/
Don’t see why not, imaging is through a regular coverslip.
Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.
'Innovation does not occur in a vacuum. It requires raw materials: the findings of basic research. We are currently burning through a stockpile of knowledge accumulated during a more enlightened era of public investment, while allocating insufficient resources for replenishing it.'
Well … we are both definitely in there
Yes, especially for flat things like cell monolayers or your hand 😜
No, this was me messing around independently. Only deskew; no rotation. It is actually surprising how little difference it makes.
Dilute more than recommended. At least 1:4000. Probably even more for the SPY-DNA dyes.
... and a projection perpendicular to the light sheet, so not truly a view from the top but at 38 (or 52) degrees ... this is still giving me a headache.
SPY-555 DNA and SPY-650 FastActX ...
well ... maybe, if someone invites me 🤪. I am realizing that one full scan of three channels of a relatively flat volume is 1GB ... this is going to present some challenges for time-lapse.
Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.
Yep. That’s the question. Tempted to test with nanocages because it it is really this bright and stable would be very useful… however, @erin-rod.bsky.social, is this thing a dimer?
Intriguing …
Finally having some time to play with ‘Snouty’ again. Thanks a million once more @tanner-fadero.bsky.social and @amsikking.bsky.social
Vaccine samples from the 1880s in Louis Pasteur’s laboratory in Arbois … Vaccines work!
Bluetorial: Non-Competitive Renewal (Continuation) Awards
Normally, multiyear NIH grants are paid with awards each year, pending administratively reviewed progress reports. “NIH” has been slow in making these awards. This has led to $1.83 B being withheld from institutions through February 2025.
Got stentor … thanks @wallaceucsf.bsky.social
At the North Bay Science Discovery Day today in Santa Rosa
Crowd is forming in SF #standupforscience
