4/4 This work was a true team effort between @amolf-nl.bsky.social, IHB Basel, and the @Hubrechtinstitute.bsky.social — led by @danielkrueger.bsky.social and Kasper Spoelstra. Huge thanks to all collaborators for making it possible.

3/4 This reveals a safeguard mechanism: epithelial cells constantly test each other’s mechanical strength. Too little (or too much) force destabilizes the barrier, linking extrusion to diseases like tufting enteropathy. Gut renewal is an active, force-regulated process.

2/4 Using live imaging, CRISPR myosin reporters, organoid villus substrates & optogenetics, we found that myosin-driven tissue tension—not compression—controls extrusion. Cells with reduced contractility are preferentially removed.

Epithelial tension controls intestinal cell extrusion Cell extrusion is essential for homeostatic self-renewal of the intestinal epithelium. Extrusion is thought to be triggered by crowding-induced compression of cells at the intestinal villus tip. In th...

1/4 New in Science: Gut renewal is not passive “crowd control.” Instead of being pushed out by crowding or dying from apoptosis, cells compete in a mechanical tug-of-war. Weaker cells are eliminated—reframing intestinal homeostasis as a force-regulated process.
www.science.org/doi/10.1126/...

2/4 Using live imaging, CRISPR myosin reporters, organoid villus substrates & optogenetics, we found that myosin-driven tissue tension—not compression—controls extrusion. Cells with reduced contractility are preferentially removed.

Do you want to learn how to make mouse thymus organoids?

Our lab recently published a STAR Protocol on generating thymic epithelial organoids from adult mouse thymus tissue.

Read it here: doi.org/10.1016/j.xp...

#Thymus #Organoids #STARProtocol #TEC

Our PhD student @samwillemsen.bsky.social presented our work on thymus organoids at this year’s Global Thymus Network meeting, ThymUS 2025.

#ThymUS2025 #Organoids #Thymus

The Clevers lab is in Spain! Our postdocs Hanxiao, @sarinashabso.bsky.social, @amandaanderssonr.bsky.social and a PhD student @katarinabalazova.bsky.social are taking part in an @embo.org Workshop on Liver and Pancreas in Metabolic Diseases in Girona 🌞 #embo #liver #pancreas #girona

IFN-responsive intestinal BEST4/CA7+ cells are targets of bacterial diarrheal toxins @cp-cellstemcell.bsky.social @thecleverslab.bsky.social
www.cell.com/cell-stem-ce...

Organoid drug profiling identifies methotrexate as a therapy for SCCOHT, a rare pediatric cancer
www.science.org/doi/10.1126/...
@thecleverslab.bsky.social

A Novel Three-in-One Pancreatic Organoid An innovative organoid model mirrors the pancreas' complexity, generating all three key cell types to explore development and possible regeneration.

Check out this new article highlighting our postdoc @amandaanderssonr.bsky.social ’s work on a novel pancreatic organoid model that includes all three key cell types, offering new insights into pancreatic development: www.the-scientist.com/a-novel-thre...

📢New human fetal pancreatic organoid model generates all three key cell types of the pancreas. New paper in @cellpress.bsky.social by @amandaanderssonr.bsky.social , @hansclevers.bsky.social and collaborators. See the article here: www.sciencedirect.com/science/arti...