Now available - Nanosoft FIB Autogrid Rings - The ring design offers major technical & usability improvements over other available FIB rings - For example significant increase in the area of your grid accessible for milling with the ion beam - Learn More zurl.co/hLTVf
Let's get ready for a full month of training in image analysis in @pasteur.fr , this summer!
Are you interested in learning how to do bioimage analysis? For light microscopy? for cryo-EM? And for cellular cryo-EM?
Stay tuned, Pasteur offers access to THREE courses, this June.
👇🎓
Figure 1. Cryo-EM of abundant protein complexes in native membranes.
Figure 2. Cryo-EM of membrane proteins in vesicles.
Figure 3. 3D reconstruction of V-ATPase in native synaptic vesicle membranes.
Figure 4. Generation of membrane vesicles for structure determination of proteins in their native lipid bilayer.
I've written a review on what I think is an extremely exciting direction in cryo-EM:
Cryo-EM of endogenous membrane proteins in their native lipid bilayer
Open access in Quarterly Reviews of Biophysics:
doi.org/10.1017/S003...
Thermo Fisher’s new Cryo-EM Drug Discovery Center in the Bay Area gives biotech and pharma teams shared access to advanced cryo-EM tools and expert support.
corporate.thermofisher.com/us/en/index/...
Hello BlueSky Community, do you know of any European Cryo-EM facility which possess a High-pressure freezer being used under BSL-2 safety level? The sample would be a bacterial biofilm.
Q-score as a reliability measure for atomic coordinate models
Q-scores are calculated for atomic models derived from 3D electron microscopy maps, measure how well the model fits the map and reflect the quality of the map itself.
🔗 Find out more:
https://ow.ly/4Vkx50Ynrxi
@ucsandiego @ActaCrystD @IUCr #CryoEM #QScores #Validation
Register: March 26 Webinar on the New PDB Beta Archive
Learn how to become an early adopter of the future PDB
The new directory structure is organized around extended PDB IDs and will replace the current archive when all 4-character PDB IDs are assigned
New preprint by @alok-bharadwaj.bsky.social explaining the workings of SURFER, a simple tool to segment membrane, micelle or nanodisc densities in cryoEM maps.
Try it via the @chimerax.ucsf.edu ToolShed:
cxtoolshed.rbvi.ucsf.edu/apps/chimera...
Despite LocScale in the name, it works on any map.
We’ve added our latest webinar to the product demos and technical presentations on our Videos page. Explore recordings covering our cameras, workflows, and real-world research applications.
Visit: https://directelectron.com/videos/
Question for the #cryoEM crowd: have you played with ModelAngelo, CryoAtom and EMProt, and would you care to offer thoughts/notes on the comparison? I have ModelAngelo installed and I love it, but the other two claim improved performance - which should I try first?
Since last year, I've been making a RELION GUI that supports viewing, navigating and running jobs in a way completely compatible with the original RELION pipeline. Now it's working nicely for both SPA and tomography! Please try it out - github.com/hanjinliu/hi...
Trimbody with rigid AI-designed scaffolds enables atomic-resolution cryo-EM structure determination of small proteins pubmed.ncbi.nlm.nih.gov/41735342/ #cryoEM
ChimeraX screenshot showing CubeNTube plugin.
The new ChimeraX CubeNTube plugin allows erasing parts of maps using cube, cylinder, and custom shapes and has undo. Created by Tamino Cairoli. Available from ChimeraX menu Tools / More Tools....
"QuickStainer": a rapid negative staining device for improved preservation of molecular structure pubmed.ncbi.nlm.nih.gov/41730344/ #cryoEM
Hassan Zafar et al.: Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies #CryoElectronMicroscopyCryoEM #StructuralBiology #SingleParticleAnalysis @uvmvermont...#IUCr https://journals.iucr.org/paper?S205979832600080X
Henry N. Jones et al.: Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements #ContrastEnhancement ... #IUCr https://journals.iucr.org/paper?S2059798326001166
Cheat sheet for ChimeraX.
ChimeraX cheat sheet brought to you by Alejandro Pereira. www.rbvi.ucsf.edu/chimerax/dat...
Missed our recent webinar on the DE API for Direct Electron cameras and scan controllers? The recording is now on YouTube! Explore integration strategies and advanced workflow control with R&D Scientist Dr. Carter Francis. View the full recording: https://youtu.be/kJi1kLvHcGo
The amyloid packing difference (APD) is a new metric to compare amyloid structures. Analysis of disease-specific structures provides context for the interpretation of APDs for new comparisons. I hope it will be useful! 🤗
www.biorxiv.org/content/10.6...
The next #CryoEM Current Practices Webinar speaker is Vladimir Kolyadko from Children’s Hospital of Philadelphia speaking on "Cryo-EM of Liposome-Assembled Macromolecular Complexes to Reveal Protein-Membrane Interactions in Hemostasis" 2/26/2026 at 12PM ET / 9AM PT
us02web.zoom.us/webinar/regi...
Pulsed #lasers integrated with #cryoEM: @fitzpatricklab.bsky.social postdoc Daniel Du aims to boost contrast in #cryoET zuckermaninstitute.columbia.edu/making-world-s-best-microscopes-biology-better
zuckermaninstitute.columbia.edu/science-life-daniel-du
elifesciences.org/reviewed-preprints/109793v1
Please spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳
mrc.tal.net/vx/mobile-0/...
A gallium arsenide hybrid-pixel counting detector for 100 keV cryo-electron microscopy pubmed.ncbi.nlm.nih.gov/41680324/ #cryoEM
By 2028, all 4-character PDB IDs will be exhausted.
After that, all new entries will receive extended IDs: 12 characters total, formatted as pdb_ + 8 alphanumeric characters (pdb_1000axyz)
Test it from PDB Beta Archive
Read more: www.wwpdb.org/news/news?ye...
Calling all microscopy enthusiasts! I have been working with Gail McConnell to coordinate the life-science section of the 21st International Microscopy Congress taking place in Liverpool, UK this September. We have some great symposia lined up!
Registration and abstracts 👉 www.imc21.org.uk
AlignPCA-2D: PCA-Reduced Euclidean Vector Alignment for 2D Classification in Cryo-EM https://www.biorxiv.org/content/10.64898/2026.02.11.705027v1
TANGO: Analysis and curation of particles in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/41667456/ #cryoEM
If you missed Alice-Roza Eruera's January webinar on Building CryoET Training Workflows Using the SBGrid Ecosystem, you can now find it on our YouTube channel.
Here: buff.ly/CPcy0Nz
#SBGrid #Webinars #ScienceMatters
Cryo-electron tomogram on a navy background with text: Cryo-ET Data Processing Workshop. June 7-11, 2026
Join us this summer for U-M's 2026 #CryoET Data Processing Workshop!
This four-day workshop will introduce participants to standard image processing packages and all aspects of cryo-ET data processing.
Apply by March 20: myumi.ch/lsi-cryo-workshop-2026
@jbquerido.bsky.social @shyamalm.bsky.social
Now available - Nanosoft FIB Autogrid Rings - The ring design offers major technical & usability improvements over other available FIB rings - For example significant increase in the area of your grid accessible for milling with the ion beam - Learn More zurl.co/hLTVf
