Joey Tidei

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

🟢FastAct™ goes green! 🟢🔬

We launch SPY488-FastAct™ a green channel live cell actin probe

SPY488-FastAct allows imaging F-actin in live and fixed cells

Compatible with SPY555 and SPY650 for easy 3 color imaging.

spirochrome.com/product/spy4...

available at our distributors in a couple of days. 🧪

Up close and personal with a branching neurite in a DIV2 hippocampal neuron expressing FTractin-GFP. This was imaged every 6 seconds over 10 minutes on the trusty @zeiss-microscopy.bsky.social LSM880. Happy #FluorescenceFriday

Also looks like it has some actin within it as well. You've really got me wondering now 😂

Although the final frame looks like it may have some attachment, or just a coincidental new branch forming in the same area.

That's a great question, I have no idea lol. I'm only collecting one z-plane here so its possible if its a neuronal structure I could be missing its attachment to the neighboring neurite.

Thank you!!

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

Thank you! And I bet you have some idea.... ol' reliable. SiR-Tubulin and SPY-FastActX 👍

Cell body getting taken for a ride in this early stage neuron. Microtubules🔵 and Actin🟣 labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday

So excited to share the preprint with the bulk of my PhD work in the Beach @myosincity.bsky.social and Oakes @pwoakes.bsky.social Labs at Loyola University Chicago! We investigate the structure, recruitment, and function of septin networks around the nucleus. A thread:
doi.org/10.64898/202...

a woman in a suit and tie has the words wait what written on her face ALT: a woman in a suit and tie has the words wait what written on her face

Two new 🔥pre-prints 🔥 TODAY by us & @pwoakes.bsky.social @myosincity.bsky.social
change the way we think about the septin cytoskeleton

Septins buffer mechanical stress on actin and membranes!

Septins are like intermediate filaments

www.biorxiv.org/content/10.6...

www.biorxiv.org/content/10.6...

Thank you!

I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

Microtubules 🟢 protruding into the dynamic actin 🟣 network of a neuronal growth cone in DIV2 hippocampal neurons. @spirochrome.com SiR-Tubulin and SPY-FastActX working wonders on this #FluorescenceFriday

LIM Domain Proteins link molecular and global tension by recognizing strained actin in adhesions Mechanotransduction is fundamental to cell signaling and depends on force-sensitive adhesion proteins. How these proteins differentiate and integrate their responses to tension remains an open questio...

Our new preprint is out! Led by @stefanosala89.bsky.social sky.social we investigate the difference between molecular tension sensing and global tension on the focal adhesion. Follow along for a thread of our findings:
www.biorxiv.org/content/10.1...

A day ahead of #FluorescenceFriday, a (temporal) switch from the bulb to the cortex.

🟣 NeuN
🔵 AnkG

🔬🧠🧪

Imaging neurons quickly for 3min typically won't give you huge cell-wide cytoskeletal events, but you do get to capture fun little tugs and loops in the soma and neurites (as well as whatever the hell is going on in the bottom right).

Stain: @spirochrome.com SiR-Tubulin

Happy #FlourescenceFriday!

Myosin 2 – A general contractor for the cytoskeleton Cells derive their shape, and in turn much of their behavior, from the organization of the cytoskeleton. While a myriad of proteins contribute to the …

Did you wake up this morning and think "Huh, I'd love to learn more about how myosin 2 filaments assemble and their contribution to cell architecture and function"? What a coincidence, I've got just the review for you.

Our PhD student Louis Romette is optimizing long-term live-cell imaging. He just casually dropped this 65-hour movie of a growing neuron (div 3 to 6) with knocked-in actin 🔥🕶️ #realtimemicroscopy

Beautiful! What imaging media did you wind up going with?

Two months left for FASEB Conference - Septins: Spatial regulators of cell biology
Register now:
events.faseb.org/event/septin...
Come find out what these filaments do at the interface of the actin and microtubule cytoskeletons and cell membranes. You'll be hooked for life.

Thank you @daniellebeckman.bsky.social ! Obviously the same for all of your images!

Congrats to the NeuroArt Grand Prize winners!
🥇 Totally Tubular –Joey Tidei @j-today.bsky.social
🥈 Neurosphere – Adam Soos
🥉 Unwelcome Guest – Danielle Beckman @daniellebeckman.bsky.social

Thanks to all who shared their art! Want to be next? Enter our contest! 🔗 neuroart.com/annual-grand...

For this #FluorescenceFriday I share another example of the possibilities of making art with imageJ processing. This is a 3 day long time-lapse of a growing slime mold, with 15 min growth waves color coded.
the white things are oat flakes. I'll share the original video in comments

#Sciart

Almost forgot to mention.... @kwolbachia.bsky.social Citynight LUT strikes again!

I've been lacking on #FluorescenceFriday the past month or two, but winning the annual MBF NeuroArt competition was a lovely surprise to end the week. Thank you @mbfbioscience.bsky.social for the recognition!

neuroart.com/annual-grand...

Unfortunately it is. Happy to see ideas being thrown around here though lol

I do most of my troubleshooting imaging with NBM/B27 but if I need good clear imaging I would use NEUMO/SOS. I'll have to check but I believe the longest I did with that media was overnight and it was definitely reduced autofluorescence. Relatively new, now relatively old I guess.

Following since I've just been informed NEUMO/SOS is discontinued 😧

The world desperately needs more microscope merch. I’d spend half my stipend.