Alessandra Boletta

𝗠𝗲𝗲𝘁 𝗼𝘂𝗿 𝗸𝗲𝘆𝗻𝗼𝘁𝗲 𝘀𝗽𝗲𝗮𝗸𝗲𝗿𝘀 𝗳𝗼𝗿 #𝗖𝗶𝗹𝗶𝗮𝟮𝟬𝟮𝟳!

We are grateful for their participation and look forward to welcoming the cilia community on 8-12, March 2027.

Wishing you all a happy and inspiring 2026! 🎉

#Cilia #Milan

PKD disease of the month!

We were asked to summarize PKD in 2000words+bullets 😰 hope it is ok thanks to wonderful infographics from Sara Clerici!

Thanks 🙏 @cp-trendsmolecmed.bsky.social for inviting us

Cilia2027, the 7th European Cilia Conference to be held in Italy from March 8th- 12th, 2027.

🌍 Cilia2027 | Mar 8–12, 2027 | 📍 Stresa, Lake Maggiore, Italy

Join the global cilia community for science, collaboration & discovery (+ fun!!).

#Cilia2027 #CiliaBiology

Top: lf5 mutant cells untransformed, or expressing CDKL5-GFP, CDKL5K33R-GFP, CDKL5S162A,T164A,Y166A-GFP, or CDKL5Y166F-GFP, and lf2 mutant cells expressing CDKL5-GFP were stained for flagella (acetylated tubulin, red) and CDKL5 (GFP, green top panels, gray in bottom panels). The cell bodies of all strains are green (or gray) due to autofluorescence. Wild-type CDKL5-GFP concentrates at the basal end of the flagellar shaft (arrows). Note that all of the mutant CDKL5 proteins lack the major enrichment at the basal end of the flagellum but continue to show punctate stain along the flagellar shaft. Scale bar, 5 µm. Z projection of slices taken at 0.5-µm intervals. Bottom: Larger version of the first image in the top row.

CDKL5 Deficiency Disorder (CDD) leads to seizures & intellectual disability. This study shows that #Chlamydomonas CDKL5 kinase is activated by the LF2/CDK20 kinase; CDKL5 in turn regulates #intraflagellar transport via protein abundance & phosphorylation #cilia @plosbiology.org 🧪 plos.io/45490eP

CiliaQ Editor just received a convenient upgrade allowing to faster inspect and correct segmentations of #cilia in the #CiliaQ workflow (automatic color & BC adjustment, new functions, etc.). If you update FIJI, you automatically update to the new version. All details at github.com/hansenjn/Cil....

Advances in ciliary proteomics – towards cracking the hidden proteome code of cilia Summary: This Review explores proteomic strategies for investigating cilia, tracing the evolution from early discoveries to recent advancements enabled by proximity labeling techniques.

📣 Here it is — our overview on ciliary proteomics! Great discussions and writing with @mick-lab.bsky.social and Ronald Roepman 🙏🙏
Hope we didn’t miss anything important - apologies if we did!

#cilia #ciliopathies #proteomics
@theracilproject.bsky.social @for5547.bsky.social

tinyurl.com/yc8ztn5v

Save the date!
March 2027 on beautiful lake Maggiore right outside #Milan, are you ready for #Cilia2027? 🥳
Stay tuned for more info!

We have an open #ERC funded PhD position to work on #cilia #mitochondria #PKD and #matabolism

Deadline July 7th. Look forward to welcoming a talented PhD student to our lab in beautiful Milan

👇

How a mysterious epidemic of kidney disease is killing thousands of young men Nature - Repeated damage from extreme heat over time seems to be a leading factor causing kidneys to fail.

Repeated damage from extreme heat over time seems to be a leading factor causing kidneys to fail

https://go.nature.com/45L9NlX

Centrioles generate two scaffolds with distinct biophysical properties to build mitotic centrosomes Spd-2 organizes the assembly of two distinct centriole-scaffolds to build mitotic centrosomes in Drosophila.

🔬 Excited to share our latest paper! We’ve found how Spd-2 coordinates with Polo and Aurora A to build critical scaffolds in centrosome assembly in Drosophila. 🧬 www.science.org/doi/10.1126/...

Cross-organ metabolite production and consumption in healthy and atherogenic conditions An atlas mapping organ-specific metabolite production and consumption across various pathophysiological states in pigs shows impaired metabolic activity across organs by a Western diet or hypercholest...

Cross-organ metabolite production and consumption in healthy and atherogenic conditions: Cell www.cell.com/cell/fulltex...

Today "a milestone in the evolution of personalized therapies for rare & ultra-rare inborn errors of metabolism"
—the 1st human to undergo custom genome editing
—from decades of NIH funded research
www.nejm.org/doi/full/10....
@nejm.org
www.nejm.org/doi/full/10....
www.nytimes.com/2025/05/15/h...

Very happy to find our last article in Kidney International Reports online today!
#cilia #ciliopathies #kidney

schemes for curvature

How curvature affects epithelial tissues?
Answer in 5 papers! To understand this, one must first gain control over curvature.

The most logical way of doing this is through scaffolding in vitro — make a shape and attach cells.

🎉 Huge congratulations to Susan Dutcher @dutcherlab.bsky.social on her election to the National Academy of Sciences! A well-deserved recognition of her outstanding contributions to cell biology and cilia research. 👏🔬 #WomenInSTEM #Cilia

Major congratulations to our fearless leader and team captain Susan Dutcher @dutcherlab.bsky.social for being elected to the National Academy of Sciences! 🙌

Another big win for #cilia biology!!

Congratulations Susan! Super deserved!

📣 Don’t miss the review by @bolettalab.bsky.social & S. Clerici on metabolic and mitochondrial alterations in #PolycysticKidneyDisease and other renal #ciliopathies ➡️ doi.org/10.1038/s443...

at IRCCS San Raffaele Scientific Institute

BSCB GenSoc UK Cilia and Centrosome Network e-Symposia Series BSCB GenSoc UK Cilia and Centrosome Network e-Symposia Series

A week until our 51st @bscb-official.bsky.social @gensocuk.bsky.social @ukcilianetwork.bsky.social UK Cilia and Centrosome Network e-symposium: 15/04/2025 15:00-17:15 BST. Still not registered for the series- join us here: www.eventbrite.co.uk/e/bscb-genso... Free and open to all! /1

Dear cilia researchers, join us in the heart of the beautiful Vosges mountains to share your latest findings! This year, partnership with @for5547.bsky.social and keynote lectures by @micromotility.bsky.social and @mick-lab.bsky.social
Registration is now open www.cfc2025.conferences-pasteur.org

The ciliary pocket: an endocytic membrane domain at the base of primary and motile cilia Cilia and flagella are eukaryotic organelles involved in multiple cellular functions. The primary cilium is generally non motile and found in numerous vertebrate cell types where it controls key signa...

journals.biologists.com/jcs/article/...

I've been doing this for 30 years & I still can't believe that when people ask me what I do for a living I get to tell them "I'm a scientist". It feels like a kid's fantasy career that they dress up as for Halloween, like "astronaut" or "pirate". I feel very lucky I get to do this day in & day out.

Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes in C. elegans - Nature Communications Decoding the content of extracellular vesicles (EVs) is key to understanding cell communication. Nikonorova et al. used targeted proximity labeling to uncover polycystin EV cargo and cargo-loading mec...

Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes in C. elegans
www.nature.com/articles/s41...

Imaging and spatially resolved mass spectrometry applications in nephrology Nature Reviews Nephrology, Published online: 27 March 2025; doi:10.1038/s41581-025-00946-1In this Review, the authors discuss mass spectrometry (MS) imaging and spatially resolved MS approaches that are being employed in nephrology applications. They also highlight emerging MS methods and applications, as well as the integration of MS data with data from other omics approaches.

ICYMI:

Looking for a home for your #cilia submission? Still time to be part of this special issue of the Journal of Cell Science- the journal for the cell biology community, by the cell biology community! ⏰ Reach out to @lottepedersen.bsky.social or I to discuss....

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

Journal of Cell Science logo Meet the Editor at Biologists @ 100: Lotte B. Pedersen Areas of expertise: Cell biology, primary cilia, centrosomes, intraciliary trafficking (IFT), cilia assembly, signalling via cilia.

Our Guest Editor @lottepedersen.bsky.social is attending our Biologists @ 100 conference. Reach out to Lotte if you have questions about submitting your research to our special issue on cilia and flagella biology: journals.biologists.com/jcs/pages/ci...
Submission deadline: 31 March
#biologists100

In L.A. heading to Ventura Beach for the #GRC on #mitochondria. Excited to share our long-standing work on mitochondria in #PKD and our more recent discovery of the cross-talk between #cilia and #mitochondria
@erc.europa.eu

Epithelial polarization by the planar cell polarity complex is exclusively non–cell autonomous For cells to polarize collectively along a tissue plane, asymmetrically localized planar cell polarity (PCP) complexes must form intercellular contacts between neighboring cells. Yet, it is unknown wh...

How many cells do you need to establish PCP? The magic number is 3! Beautiful work by Lena Basta in Danelle Devenport's lab. Happy to have contributed. www.science.org/doi/10.1126/...

Lipoylation inhibition enhances radiation control of lung cancer by suppressing homologous recombination DNA damage repair Genetic screen finds lipoylation as a target to improve radiation success in lung cancer by blocking DNA damage repair.

1/Happy to share a new paper reporting a metabolic approach to radiosensitization in lung cancer, from Rachel Chiang, Faith Zhang and colleagues at UT Southwestern Radiation Oncology and @cri-utsw.bsky.social
www.science.org/doi/10.1126/...