Its kind of funny that some of those very basic mass spec'ing things become challenging with these advanced models.. 😂
11.03.2026 20:47
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Its kind of funny that some of those very basic mass spec'ing things become challenging with these advanced models.. 😂
Setting up a decent DDA method on Astral is so mentally taxing.. It isn't meant to follow any of the data dependency criteria.. Enforcing it to follow the criteria is where it gets taxing.. Wonder how it is working for those MS2-TMT workflows.. 🤔🤔
It is an incredible honour to be featured in the Faces of Mass Spectrometry series of JASMS and share the story of a nerdy mass spectrometrist.. who despite being an introvert, always love to talk about how wonderful mass spectrometry is.. ☺️☺️
Proud to be a part of #TeamMassSpec and #FeMS.. Thanks..
Today at 13:50, on the dot, the sequences running on both Ascend and Eclipse came to an abrupt stop.. Just at random.. In the middle of a run.. Neo kept running but no MS acquisition.. Had to restart Xcalibur and reconnect LC to resume the sequence.. Very spooky!!! #MassSpecMusings
That sounds like an idea.. Thanks..
Anybody doing proteomics on FACS sorted cells? People are using BSA coated tubes to avoid cell adhesion onto the walls. BSA is all over the protein extract. How to avoid this BSA? Saw Polyvinyl alcohol (PVA) can be used in place of BSA. Any experience with its compatibility for downstream MS? Thanks
"This work provides insight to help future proteomics study designers make more informed decisions on whether physical normalization is necessary for their experiments"
Guess I am that old rigid mass spectrometrist now who finds it hard to keep an open mind to new concepts.. 😉😉
I have all these glycan specific fragments from a glycopeptide analysis.. Any software suggestions for identifying both the peptide and the attached glycan composition from such spectra?? Thanks very much.. #glycotime #TeamMassSpec
Thanks.. Have seen a few studies reporting proof of principle with MS3 strategies and MS2-MS2 ETD strategies with cleavable crosslinkers.. Any comments on that??
The interest here is exploratory where the subunits in complex are uncharacterized..
I am actually looking for the latter option.. The crosslinked peptides need to be identified by MS for confirmation.. Any suggestions?? Thanks..
#TeamMassSpec Question for XL-MS practitioners.. Looking for a suitable crosslinker to stabilise a protein complex in vitro.. What are the pros and cons for MS-cleavable vs non-cleavable cross-linkers?? Is PhoX a good choice?? Any recommendations for MS-cleavable cross-linkers?? Thanks very much..
The Spatial Omics Summer School offers a structured, hands-on introduction to spatially resolved targeted transcriptomics, proteomics, and metabolomics.
If you want to join, submit your application by 2 February 2026.
https://www.vibconferences.be/events/spatial-omics-summer-school
King's college London
Apparently this is exactly what happened with the Lumos in my facility a few years back.. Yup, had to get it replaced..
It's almost a futile effort for people who have to go through a visa stamping process aiming to attend ASMS now.. Hopefully, the conference will be hosted in Canada again at some point..🤞🏾🤞🏾🤞🏾
(J Proteom) Two-dimensional electrophoresis-based proteomics reveals soybean seed hypocotyl proteoforms: Publication date: Available online 17 January 2026
Source: Journal of Proteomics
Author(s): Jian-Zhong Tan, Hiroyuki Kagawa, Keiko Kizawa, Hisashi Hirano #MassSpecRSS
Peptide:TMT ratio and pH are major factors influencing labeling efficiency.. Ensure both variables are controlled.. Could inject a labeled sample before pooling to quantity labeling efficiency.. Use TMT as a dynamic mod for this.. ideally up to 98% labeling on K and ~95% on N-term is anticipated..
Big evaluation of our Orbitrap Astral Zoom mass spectrometer by the Olsen group, now peer reviewed, published and open access.
www.sciencedirect.com/science/arti...
(JASMS) [ASAP] Faces of Mass Spectrometry/Khatereh Motamedchaboki: Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00430 (RSS) #MassSpecRSS #JASMS
I am a fan of TMT. There is no reason to feel intimidated. It will be alright. Follow the protocol. Design the experiment carefully and follow the steps diligently. Ensure normalisations and labeling efficiencies are checked and controlled. The TMT quantitative data is awesome. All the very best.
Vanquish Duo as Neo Mono with EasySpray column setup.. Works quite alright.. Guess have to get uPAC to test the dual column setup at nano flow rate..
A slide from a presentation I gave in 2013 on mass spectrometry proteomics.. Ironically, still a relevant topic to include this slide as such in a talk I am preparing to give after the new year..
Column emitter with a weird thing stuck on..
Holidays started.. And the festive decor is on.. 🎉🎉#MassSpecHolidayDecor
Post-doc positions:
"Academic freedom is under pressure today. This requires rescue havens of free research. ... [we] invite early career researchers, whose work is restricted due to political pressure in the USA..."
uni-freiburg.de/frias/call-f...
Yup.. Forces you to be more disciplined in the lab. Get all the samples ready and load up in the autosampler before starting the sequence..
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Yup.. I am skeptical too.. testing it out to see whether it works as a single neo if the other pump and column over are removed from the configuration..
Guess at which point I realized that the column heater is not connected.. I am very glad that this is the only boss-back-on-bench catastrophe so far.. The muscle memory from years of proteomics works still.. Thankfully, nothing got blown up.. yet.. 😂😂
Damn.. Neo firmware update 2.51 locks you out of the autosampler and prevents you from opening the door once the sequence is started.. Damn..
Changed the 50um lines to 20um.. With this setup the delay is not larger than what generally is for a setup where the column oven is in use (like when uPAC columns are being in use).. Frankly, the plan is to set it up as a single neo instead of a duo for now.. Yet to test this setup though..
#TeamMassSpec Can someone please help me remember whether the Vanquish Duo launched at ASMS2024 was meant for nanoLC applications?? Somehow, I remember it as the potential high speed solution from Thermo for Astral high SPD applications.. However, the Duo I got hold of here is a microflow version!!