Silvia's simulations showed that while all epitopes expose Sia for binding, Siglec-6 recognises and binds only GM1 because of a key interaction with the membrane through W127 and K126, which orientates the V-set domain to bind the Sia through Arg122 and the terminal Gal to the C-C' loop 😎
Structure of a protein (cyan/teal) rendered as surface with 50 frames representing the dynamic ensemble of the glycans (white surface and sticks) depicted at the three sites (N93, N99 and N104) indicated by the 2D SNFG symbols and corresponding GlyTouCan IDs. Graphic rendering with VMD
Happy new year 2026 #glycotime! 🥳 We just introduced some useful options to Re-Glyco Ensemble.
In the Advanced Settings you can now select a seed (positive integer) to reproduce structural ensemble of your favourite glycoforms such as the one below that you will see soon in an upcoming paper,
Super interesting work from @carolynbertozzi.bskyverified.social group about the effects of glycosylation in protein structure and function at the proteome scale 🤯💥🤩
Results show that sialylation and fucosylation are crucial, which makes a lot of sense. We did some extra bits 🧐 with GlycoShape ⬇️🧵
Front page of Glycoshape website with the new background with floating SNFG symbols representing different monosaccharides. In the website the background is animated, so check it out at https://glycoshape.org
When you rebuild a glycosprotein with Re-Glyco Ensemble, now you can download the multiframe PDB with SASA values in the B-values column. The structure on the left shows in blue all the accessible surface and in light blue and red inaccessible residues due to the glycan presence. The buttons below are in cyan for regular multiframe PDB download (left) and download of all the file jobs (right) the middle gradient colored button allows users to download the PDB with SASA values
We just did some maintenance and updates to glycoshape.org,
✅ Fancy background with floating SNFG symbols 🤩
✅ 'Latest Updates' feed from this account on bsky 🤩
✅ Download PDB with SASA values in Re-Glyco Ensemble 🤩
Check it out!
Hello #glycotime! GlycoShape makes its debut on bsky today 🦋🎉 Follow us for news, updates and fun-facts (I take full responsibility for those, Ojas @ojas-singh.bsky.social 😆) below ⬇️
Super excited by our new #glycotime preprint! A long-term project from my group with awesome collaborator @elisafadda.bsky.social
Regulation of N-glycosylation efficiency by eukaryotic oligosaccharyltransferase www.biorxiv.org/content/10.1...
thread below 👇
Schematic representation of the 14 human siglecs, 4 evolutionarily conserved on the left, with low sequence similarity, and 10 on the right sharing a higher sequence similarity to CD33. Siglec-6 is highlighted in yellow as it is the focus of this work. This is Figure 1 in the paper
When glycan recognition is linked to immune response, you need fine tuning! 😎
Here ⬇️ we show how human Siglecs act as molecular precision tools, recognising the glycan and also the biological environment (or context) these glycans are found in. A 1/5 #glycotime🧵 ⬇️
doi.org/10.1101/2025...
Registration to our "Structural Glycoscience" Summer School is open !! Come to Grenoble for #glycotime lectures, practicals, visit to @esrf.fr @cermav.bsky.social and IBS. Information and registration at glycoalps.univ-grenoble-alpes.fr/news/summer-...
Glycoshape is 1 year old! 🎂🎁 🎉 Last year today we launched glycoshape.org after extensive consultation with a team of friends and colleagues. As head chef, it is my pleasure and duty to give a few thanks before the toast🥂 1/3
Cover of Nov 2024 issue of Nature Methods (not our cover) showing an image of a section of a mouse intestine mostly yellow and green and cyan at the top, black background
Front page of our article
Figure 1 (left) and figure 2 of our article
Christmas arrived early for me🎄🎁 just got a hard copy of the Nature Methods Nov 2024 issue with our GlycoShape article in it ❤️❤️ it smells sooo nice!!! 🤩🤣
doi.org/10.1038/s415...
Announcement of best posters awards at the SfG2024
And the best poster award #SfG2024 goes to.....Ojas Singh @ojas-singh.bsky.social for his incredible work on GlycoShape!!!! 🎉🎊🎉🎊🎉🎊 Super congrats Ojas we are suuuper proud of you! #glycotime
Thanks @elisafadda.bsky.social 😸
