@hugovdhoek
Cryo-ET of eukaryotic cells & cilia 🔬👨🔬 Looking at tiny slices of frozen cells in 3D🦠 Former Engel Lab, Biozentrum, Uni Basel 🇨🇭🏡 Metalhead, he/him. Nature is hella strange and I'm all here for it 🤓🕵🏼♂️
This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell!
This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍
[Maybe long thread ahead]
C2CD3 localizes to a ring structure observed in the lumen of the distal centriole by in situ cryo-electron tomography. Top: Confocal image of an expanded mouse photoreceptor cell immunolabeled for tubulin (magenta) and C2CD3 (green). DC, daughter centriole; BB, basal body; TZ, transition zone; CC, connecting cilium. Scale bar: 500 nm. Middle: In situ cryo-tomogram slices taken along the longitudinal axis of the centriole and connecting cilium. White arrowheads indicate microtubule triplets, black arrowheads indicate microtubule doublets, orange arrowheads mark the luminal ring structure, and yellow highlights the membrane. Scale bar: 50 nm. Bottom: Model of the architecture of the distal region of the human centriole.
How does C2CD3 contribute to distal appendage formation in centrioles? @centriolelab.bsky.social show that C2CD3 acts as a central architectural organizer of the distal #centriole, bridging the luminal distal ring complex & peripheral appendage sites @plosbiology.org 🧪 plos.io/496XZuH
Woo! Very excited to share our collaborative work on the centriole's Luminal Distal Ring ⭕. This project started 13 years ago with @hugovdhoek.bsky.social & @stearnslab.bsky.social, and then beautifully came together with @computingcaitie.bsky.social & @centriolelab.bsky.social.
#TeamTomo #UExM 🧪🧶🧬🔬
We’re kicking off the DinoSphere Online Seminar Series! Join us for our first session with Karel Mockaer (Heidelberg) & Yong Heng Phua (OIST)
📅 1 July 9AM CEST
🔗 tinyurl.com/4mjaverj
Spread the word!
@protistwtmostest.bsky.social @ehehenberger.bsky.social @chandnibhickta.bsky.social&Norico Yamada
PhD Timeline xkcd.com/3081
"The only thing necessary for the triumph of evil is for good men to do nothing.”
Keeps resonating with me, painfully so. The democratic party is such a joke. They bend over backwards to republicans/MAGA to stay relevant. Idk what they're even doing right now to stop the dictatorship. Nothing?...
Right???! Total apathy. Every day further solidifies their power and authority. If I were a US citizen, I'd try to evacuate anyone I can from there, ASAP. Before they're quite literally trapped in "Techno-feudalism meets The Handmaid's Tale"
Rare cellular events observed in the cryo-ET dataset. Slices through tomograms (left) with corresponding 3D segmentation (middle and right). Right panels show enlarged views of the boxed regions in the middle panels. A) Mitochondrion at the plasma membrane, tethered to microtubules by thin filaments of unknown identity. Segmented classes: nuclear envelope (light blue), nuclear pore complex (dark blue), 80S ribosomes (orange), mitochondrial membranes (dark red), ATP synthase (royal blue), plasma membranes (grey), microtubules (pink), filaments (yellow), endoplasmic reticulum (purple). Zoomed-in panel shows thin filaments running between microtubules and the mitochondrion at the plasma membrane. Note that this tomogram contains two closely appressed cells, and thus, two plasma membranes with cell walls between. B) Mitochondrial fission event with ER membrane interactions. Segmented classes: mitochondrial membranes (dark red), ATP synthase (royal blue), 80S ribosomes (orange), thylakoid membranes (green), PSII (bright yellow), cell wall (grey), ER (purple), fission site containing filamentous structures perpendicular to the mitochondrial long axis (pale yellow). C) Ciliary transition zone between basal body and axoneme, including assembling IFT train and stellate structure77. Segmented classes: microtubule doublets (light blue), central microtubule pair (light green), stellate structure (pale red), IFT train (yellow). D) Pyrenoid tubule extending from the thylakoids into the phase-separated Rubisco matrix of the pyrenoid. Minitubules originate from thylakoid membranes82. Segmented classes: thylakoid membranes (dark green), PSII (yellow), pyrenoid tubule (lime green), Rubisco (lavender blue), minitubules (orange). Scale bars in A-D: 100 nm. Related to Fig. 2C-H.
To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽💻
www.ebi.ac.uk/empiar/EMPIA...
A little thread about what's new... 🧵 1/n
#World: :((((((((
Me: ((((:
( #Everything is a lot right now. Have a #squirrel )
The dominoes meme. The smallesr domino is a funny dog on the internet, the largest domino is the total fascist takeover of the U.S. government Made by myself
Sigh. A big warm hug to y'all. Undoing a #dictatorship will not be easy.
#Science, #Census, #USAID and many more #institutions have already fallen. Their datasets etc has been erased. No access. It's going so fast.
I wish you all the strength in the world and more💕 I'm afraid you're gonna need it
Hey #TeamTomo❄️🔬 computer wizards!👩💻🧙♂️ This is an amazing opportunity for a permanent position working alongside the fantastic Alicia Michael and Florian Schur, pushing #CryoET image analysis of chromatin and actin networks inside native cells!🧬🧶🧪 Vienna's not a bad place to be either.🍻😁
As a structural biologist there's a stage in discovery where you and your colleagues have seen an amazing structure but there's still work to do before it's ready for dissemination. You walk around knowing that every person, every living animal, carries that fascinating structure in their body.
A Reese's butter cup with scientific papers, images and data coming out of it
Please be sure to check you kid's candy this year. I just found another project I have no time for in a Reese's cup
Meet the PyShell!🥧🐚
Diatom pyrenoids fix up to 20% of the Earth's #CO2🌏. We discovered that their Rubisco is encased in a tight protein lattice that is essential for efficient carbon fixation and cell growth🌱
Super excited to share our new preprint📜: www.biorxiv.org/content/10.1...
🌾🧪 🧶🧬 ❄️🔬
Don't worry, they will have everything fixed by 2070 (not a joke) www.zdf.de/nachrichten/...
It is my pleasure to announce the @EMBO course on “In situ structural biology by Cryo-FIB and Cryo-ET” taking place @MPI_Biochem from 8.-14. April 2024. If you are interested in learning about cryo-ET and cryo-FIB including lift-out, apply by December 8th!
meetings.embo.org/event/24-in-...
Walking new people through tomogram processing:
