Bastian B.

Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity In this study, an imaging technology, “VISUN,” is developed that allows live-cell visualization of unmodified influenza virus during infection with single-vRNP resolution. Combining VISUN with multipl...

Out now in Cell Systems: We develop a technique to visualize influenza A virus in live cells with single viral RNA resolution. Combined with precise readouts of viral transcription, we map the whole influenza A viral life cycle and identify key bottlenecks in infection.

www.cell.com/cell-systems...

My guess is an mRNA with tagged with fluorogenic protein Pepper?!

Productive weeks at the Chemical Biology department at @mpi-mr @kjohnsson 🎉
Check out the fantastic work by @steffi-k on SNAP-tag2 and @deensun3 on KinProLa in @natchembio.nature.com ⏬

We also released a great collab with @zhixingchen2 on the photophysics of self-labeling tags in @pnas.org. (1/4)

Investigating kinase activity in living cells

📣Paper alert! 👏 If you're interested in investigating kinase activities in living cells, our scientists @kjohnsson.bsky.social have built a molecular recorder to monitor them spatially and temporally. Check out our website or Nature Chemical Biology (link in comments)!
www.mr.mpg.de/14652710/mol...

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:

www.biorxiv.org/content/10.1...

Congratulations to Julian Kompa and the entire team.

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

📣Paper alert! Interested in faster and brighter protein labeling than before? 👀 @steffi-k.bsky.social & other colleagues have developed an improved tool for labeling proteins with synthetic fluorophores for bioimaging. Also works in STED microscopy 🤩🔬 Congratulations!🥳
www.nature.com/articles/s41...

Please check out our new high-affinity split-HaloTag for live-cell protein labeling www.biorxiv.org/content/10.1...
Congratulations to Yin-Hsi Lin!

Improving split‐HaloTag through computational protein engineering Split-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual ....

Improving split-HaloTag through computational protein engineering:

onlinelibrary.wiley.com/doi/10.1002/...

1/5 🚀 Excited to share our latest preprint from: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 on #bioRxiv!
www.biorxiv.org/content/10.1...

#JohnssonLab and #ArndtLab with valued collaborators

Mapping the complete influenza A virus infection cycle through single vRNP imaging Cell-to-cell heterogeneity is a common feature of viral infection that can generate enormous complexity, complicating understanding of infection progression and interpretation of differences between v...

Our new paper is out: "Mapping the complete influenza A virus infection cycle through single vRNP imaging". Combining newly-developed single-molecule imaging approaches with in situ viral transcriptomics, we identify numerous non-canonical infection pathways.

www.biorxiv.org/content/10.1...

Long‐Term Single‐Molecule Tracking in Living Cells using Weak‐Affinity Protein Labeling A self-labeling protein tag in combination with exchangeable fluorophore ligands bypasses photobleaching and enables long-term single-molecule imaging in living cells. This extends observation times ...

Hi Bluesky! 👋
Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬
#ChemBio #microscopy

onlinelibrary.wiley.com/doi/10.1002/...