Klaas van Wijk Lab

Hypoxia‐activated fluorescent probes as markers of oxygen levels in plant cells and tissues Low oxygen signalling in plants is important in development and stress responses. Measurement of oxygen levels in plant cells and tissues is hampered by a lack of chemical tools with which to reliab...

And now formally published, a chemical probe that can detect hypoxia in plants - congratulations to all authors! nph.onlinelibrary.wiley.com/doi/full/10....

super important👇

Detection of human unannotated microproteins by mass spectrometry-based proteomics: a community assessment Thousands of short open reading frames (sORFs) are translated outside of annotated coding sequences. Recent studies have pioneered searching for sORF-encoded microproteins in mass spectrometry (MS)-ba...

#Microproteins may be the next frontier in #proteomics. But figuring out the publications can be tough.

If you are looking for some advice about microprotein evidence, check out the latest from our consortium. Lead by @carvunis.bsky.social and Aaron Wacholder!

🧬 💻
#genomics
#RNASky

bit.ly/4bgJSDm

Gordon Research Conference on Chloroplast Biotechnology; Engineering Plastids for Better Crops and New Functions Sun 30 Mar, 5:00 PM - Fri 4 Apr, 9:00 AM [EDT]: The Chloroplast Biotechnology GRC is a premier, international scientific conference focused on advancing the frontiers of science through the…

📅New #PlantSciEvents Event Added: Gordon Research Conference on Chloroplast Biotechnology; Engineering Plastids for Better Crops and New Functions🌱

👉https://eventform.plantae.org/plantae.calendar/detail/1367/1743368400000

#PlantScience

BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition
academic.oup.com/plcell/advan...
#plantscience

Aptamer and N-Degron Ensemble (AptaGron) as a Target Protein Degradation Strategy Target protein degradation (TPD) is a promising strategy for catalytic downregulation of target proteins through various cellular proteolytic pathways. Despite numerous reports on novel TPD mechanisms, the discovery of target-specific ligands remains a major challenge. Unlike small-molecule ligands, aptamers offer significant advantages, owing to their SELEX-based systematic screening method. To fully utilize aptamers for TPD, we designed an aptamer and N-degron ensemble system (AptaGron) that circumvents the need for synthetic conjugations between aptamers and proteolysis-recruiting units. In our AptaGron system, a peptide nucleic acid containing an N-degron peptide and a sequence complementary to the aptamer was designed. Using this system, we successfully degraded three target proteins, tau, nucleolin, and eukaryotic initiation factor 4E (eIF4E), which lack specific small-molecule ligands. Our results highlight the potential of the AptaGron approach as a robust platform for targeted protein degradation.

If you are interested in TPD tech but worried about synthesis of the compound, you can still design your own TPD agent using Aptamer and N-Degron Ensemble (AptaGron) platform. Check out our strategy from our latest study.

pubs.acs.org/doi/10.1021/...

#KoreaUniversity #PROTAC #TPD

New evidence for the presence and function of phosphoinositides (PPIs) in the chloroplast - preLights PPIs: The messengers between organelles in plant cells.

PPIs: The messengers between organelles in plant cells. 🌿

Shreya Pramanik covers work from Sedaghatmehr & colleagues #MPIMP_Potsdam exploring the lipid composition of chloroplast membranes by developing PPI biosensors. #preprint #PlantBiology

#preLight ⬇️👀
prelights.biologists.com/highlights/n...

Fluorescence microscopy of plant leaf cells showing mitochondrial and chloroplast targeting of proteins.

Second paper from the lab out today (woohoo!). This one led by Rachael DeTar. Check out what happens to mito+plastid translation machinery in plants that have lost photosynthesis!

Published in @pnas.org: www.pnas.org/doi/10.1073/...

Also available on bioRxiv: www.biorxiv.org/content/10.1...

New Insight Into Chloroplast Evolution - Astrobiology One of the most momentous events in the history of life involved endosymbiosis

New Insight Into Chloroplast Evolution
astrobiology.com/2024/12/new-... #AGU #AGU24 #AGU2024 #astrobiology #evolution #microbiology #genomics

Species-wide inventory of Arabidopsis thaliana organellar variation reveals ample phenotypic variation for photosynthetic performance | PNAS Efforts to improve photosynthetic performance are increasingly employing natural genetic variation. However, genetic variation in the organellar ge...

Species-wide inventory of Arabidopsis thaliana organellar variation reveals ample phenotypic variation for photosynthetic performance | PNAS www.pnas.org/doi/10.1073/... @pnas.org

Abstract Submission OPEN for #ICAR2025 International Conference on Arabidopsis Research!
Topics for Concurrent and Workshop sessions published!
📅 June 16-20 Ghent 🇧🇪
🕸️ icar2025.com

Happy to be here on Bluesky. We'll be doing a little series of "meet the committee members" over the next few weeks, having just held our elections for new members. Look out for the old and new faces coming soon 😊

Spatial Proteomics is the Method of the Year 2024!

Trying out Bluesky social for posting and following science news about the van Wijk lab in Cornell, novel discoveries in proteolysis & proteostasis, chloroplast biology and advances in proteomics and mass spectrometry