Matt Johnson

Our new preprint 🚨🌱☀️on flourescent biosensing of ATP levels in chloroplasts in vivo shows disequilibrium between proton motive force and ATP levels. We show PGR5 dependent cyclic electron transfer does not augment ATP/NADPH ratios @gustaf23.bsky.social 🙌

www.biorxiv.org/cgi/content/...

Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵

Structure, regulation and assembly of the photosynthetic electron transport chain Nature Reviews Molecular Cell Biology - The electron transfer chain in chloroplast thylakoid membranes uses solar energy to produce NADPH and ATP, which power carbon fixation into biomass. This...

Pleased to share my very general review of Photosynthesis now published in Nature Reviews Mol Cell Biol
🌞🌱
rdcu.be/em1lz Hopefully a useful primer for Postgrad and undergrad students

Towards climate-change resilient crops by unravelling the relationship between CO2 assimilation and ATP production at University of Sheffield on FindAPhD.com PhD Project - Towards climate-change resilient crops by unravelling the relationship between CO2 assimilation and ATP production at University of Sheffield, listed on FindAPhD.com

2. Towards climate-change resilient crops: unravelling relationships between CO2 assimilation and ATP production led by @profmattjohnson.bsky.social
Power photosynthesis for climate-smart crops. Investigate CO₂ uptake + ATP production.
bit.ly/4m5BZ8X
#PlantScience #AcademicSky

Tethering ferredoxin-NADP+ reductase to photosystem I promotes photosynthetic cyclic electron transfer The location of the key photosynthetic enzyme FNR impacts the partitioning of photosynthetic electrons between the linear and cyclic electron transport pat

Finally! After a lot of hard work our paper on tethering FNR to PSI in Chlamy is out in @theplantcell.bsky.social
@sheffieldpps.bsky.social @profmattjohnson.bsky.social

academic.oup.com/plcell/advan...

A family of NADPH/NADP+ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes - Nature Communications This article presents NAPstars, a family of genetically-encoded biosensors that enable real-time monitoring of NADP redox dynamics across species. The sensors reveal robust NADP redox regulation, cell...

A family of NADPH/NADP+ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes www.nature.com/articles/s41... @naturecomms.bsky.social

Determining role of FNR location in photoprotection of photosystem I https://buff.ly/4imFBBM
Life depends on photosynthesis; you will study a key photoprotective regulatory mechanism controlling electrical current flow between photosystems
matt.johnson@sheffield.ac.uk @profmattjohnson.bsky.social

Molecular architecture of thylakoid membranes within intact spinach chloroplasts Thylakoid membranes coordinate the light reactions of photosynthesis across multiple scales, coupling the architecture of an elaborate membrane network to the spatial organization of individual protei...

www.biorxiv.org/content/10.1...

What’s 7 years among friends? 😂

Molecular architecture of thylakoid membranes within intact spinach chloroplasts Thylakoid membranes coordinate the light reactions of photosynthesis across multiple scales, coupling the architecture of an elaborate membrane network to the spatial organization of individual protei...

This one was delayed for a while… apologies to the musicians. Collab with Matt, Will and Lorna (over on Twitter). And a great dev effort from Lorenz @lorenzlamm.bsky.social. Ofc @cellarchlab.com team. Comment if you want to help. #teamthylakoid

www.biorxiv.org/content/10.1...

It took us seven years, but our collaboration with @profmattjohnson.bsky.social on the molecular architecture of plant thylakoids is finally online. Check it out! #PlantScience #TeamTomo 🧪🔬🧶🧬🌾

🥳New cryo tomography Preprint ☀️🌱🔬Molecular architecture of thylakoid membranes within intact spinach chloroplasts.

doi.org/10.1101/2024.1…
Proud to be part of this great work with
@cellarchlab.com
@wojwie.bsky.social
@sheffieldpps.bsky.social

Two great #PhDposition available in the Photosynthesis Research Group at Sheffield 🌞🌱🦠 Pls RT
tinyurl.com/5yhajrpp
tinyurl.com/4nsdu45s
Come and join us!

www.biorxiv.org/content/10.1...

Great to see our paper on tethering FNR to PSI in Chlamydomonas out as a preprint @sheffieldpps.bsky.social