German train? Very brave! 😉
02.12.2025 06:37
👍 1
🔁 0
💬 1
📌 0
German train? Very brave! 😉
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data.
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
The Dutch mountains…
Finally, an FSC discussion again! It’s been a while…..
Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
Last day of a fantastic Inserm tomography workshop in Bordeaux.
Oranjerie Roermond tonight.
Cold showers and warm beer the rest of the week at CCPEM!
Mark? @subangstrom.bsky.social
Ever wondered how some of the automation in cryo-EM data collection works? Read Bram Koster's 1989 thesis!
repository.tudelft.nl/record/uuid:...
I like the genre!
Take a look at my personal perspective on the past, present, and future of in situ structural biology with cryo-ET+
While initially skeptical about the genre, I found it fun to write and hopefully, you can enjoy my experiences and some thoughts. Nice weekend y'all
www.cambridge.org/core/journal...
Looking for a primer on why the contrast transfer function matters in #cryoEM?
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
Phase plates: what do we expect from the next attempt, the laser phase plate?
As long as it’s not past 2/3 Nyquist, nobody cares.