Daniel von Wangenheim

🔊A long time ago in a microscopic galaxy far, far away....

🎬Episode IV - A NEW MICROSCOPE 🔬
"It is period of cellular rebellion. Arabidopsis root cells have won first victory against the dark forces of DNA damage"

Thanks to @dvonwangenheim.bsky.social and new i3 spinning disk @the.3i.social

Thank you @dvonwangenheim.bsky.social @the.3i.social for this awesome demo of your spinning disc system for #rootimaging

Intelligent Imaging Innovations - Microscopist Intelligent Imaging Innovations

3i is hiring a Microscopist - repost & tell a friend!
Ideal candidate has experience in fundamental optical theory & familiarity with lightsheet, spinning disk, multiphoton, TIRF etc. Full-time, exempt position (travel to visit customers) offering a competitive salary & comprehensive benefits.

Visit our booth #818 at #CellBio2025 feat. Marianas SDC SoRa with Vector3 TIRF.

Arc TIRF, ring TIRF, FRAP & widefield all in one device with intuitive control powered by Synergy for SlideBook.

And enter to win our new pRImr device! (Link below)

#microscope #automation #superresolution

New 3i PlantScope in the wild! Can't wait to see beautiful root and shoot tracking data from your new toy.
#3iPlantScope @the.3i.social

Happy to contribute to the analysis of α5β1 integrin using @the.3i.social LLSM
@natcomms.nature.com

rdcu.be/eNeNh

@ludovicleconte.bsky.social M. Shafaq-Zadah @christianwunder.bsky.social J. Salamero, Johannes team
@institutcurie.bsky.social #inria @france-bioimaging.bsky.social @inserm.fr @cnrs.fr

Fantastic use of @the.3i.social #latticelightsheet microscopy in this multi-disciplinary project digging into the literal ins and outs of α5β1 integrin. Great work y'all!

Latest imaging data from our completely re-designed ExA-SPIM system.

Expansion-assisted imaging (3X expansion, 0.3 NA) enables brain-wide imaging with high-contrast and <400 nm effective resolution.

Thank you @paveltomancak.bsky.social @olafselchow.bsky.social @dvonwangenheim.bsky.social @bundschuhst.bsky.social @ereynaud.bsky.social @cuencam15.bsky.social Vlado, Stephan for a life-changing lightsheet experience towards even more beautiful and high-quality science

... and @dvonwangenheim.bsky.social's great video montage of a few of the samples. #LISH25
🔬🌍: www.intelligent-imaging.com/AxL

Huge thanks to @paveltomancak.bsky.social and @embo.org for hosting this year's amazing Light Sheet Microscopy course #LISH25. Our @dvonwangenheim.bsky.social and team were thrilled to capture beautiful images of many samples with AxL Cleared Tissue LightSheet 🔬
🌍 www.intelligent-imaging.com/AxL

#LISH25 final student team presentations. #Specimen_Illuminati

#LISH25 final student team presentations. #SPIMply_the_Best

#LISH25 final student team presentations. #Liv_and_Dye

#LISH25 final student team presentations. #The_Holy_de_Broglies

An AD mouse brain with therapeutic antibody (in blue) and smooth muscle cells (in yellow).The first attempt, but quite successful. Thanks to @dvonwangenheim.bsky.social
#LISH25

The world behind LS7 and Blaze starts with 3i for me. Very excited to see the result of an overnight imaging #LISH5 #AD #neuroscience

Super excited to show you our solutions for vertical plant microscopy at 3i @the.3i.social
🌱🔬

Or just swing by and say hello to Ben @bta3i.bsky.social and Daniel @dvonwangenheim.bsky.social
🙂

#ICAR2025

🔬data courtesy of @trevormnolan.bsky.social @xiaohuili.bsky.social - thank you!

Cell Division. Elongation. Differentiation.

Capture the full spatiotemporal dynamics of root development with PlantScope automated montage-tracking!

See 3i's @dvonwangenheim.bsky.social present at #ICAR2025 Friday June 19 at 9AM

🌍: www.intelligent-imaging.com/plantscope

🔬data courtesy of @trevormnolan.bsky.social @xiaohuili.bsky.social - thank you!

🌱 Excited to share that I will start my lab at EPFL, CH this June supported by a @snsf.ch Starting Grant! ramakrishna.ch

We are interested in plant mineral nutrition and will deep-dive into the fine-tuned mechanisms employed by plants to adapt to abiotic stresses such as salinity.

#newPI #plantsci

🌱 When a root cell undergoes symmetric division, are the daughter cells actually identical? By combining live-cell imaging and scRNAseq we discovered a new cell state with uneven BR activity. Read our full paper in Cell: doi.org/10.1016/j.ce... @nvukas.bsky.social @trevormnolan.bsky.social

🌱 Thrilled to see our latest collaborative work with @jennyrussinova.bsky.social @nvukas.bsky.social published in Cell! By combining single-cell RNA-seq and live imaging, we uncovered previously hidden brassinosteroid signaling states and their role in optimizing root growth. doi.org/10.1016/j.ce...

It's #3DThursday!🔬🌈 We had a great week at the Harvard Tissue Clearing Workshop, huge thanks to:
@edwardlachica3.bsky.social
@the.3i.social
@harvardmed.bsky.social
@bostonchildrens.bsky.social

Imaged: Dan Giardina & Ed Lachica, 3i
Visualized: syGlass.io
Sample Prep: Dharmendra Puri, HMS, BCH

Tried a new fixation method for 3D imaging of clear tissues using iDISCO. What a difference! 4% para on the left. New method on the right.
Stained MBP in magenta and Veins in green. Images captured using AXL from 3i. @edwardlachica3.bsky.social
@dvonwangenheim.bsky.social @samuelconnell.3i.social

@the.3i.social is ready to roll with its #lightsheet #microscope at @paulamonterollopis.bsky.social Tissue Clearing workshop #TCW2025 See you Monday!

Oh, just playing around with the #AllenBrainAtlas in #SlideBook for #FluorescenceFriday...

🔬 www.intelligent-imaging.com/AxL
🧠 brain-map.org
🖥️ www.intelligent-imaging.com/SlideBook

Give SlideBook Scripting Superpowers! 🦸‍♂️🖥️🔬🧠

Announcing two new modules for SlideBook #microscopy #software: Union and Synergy. Implement analysis and imaging pipelines best suited for your science including machine learning and deep learning

🌎: go.intelligent-imaging.com/SlideBook-Un...