Filipe Carvalho

@attychang.bsky.social, @nanigrotjahn.bsky.social et al. show that cytoplasmic #ribosomes on #mitochondria alter the local membrane environment for protein import. rupress.org/jcb/article/...

📕 From The Year In Cell Biology: rupress.org/jcb/collecti...
#CellBio2025

Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes - Nature Communications B. thuringiensis spores contain uncharacterized protein filaments that extend from the surface of the exosporium. Here, the authors show that these filaments feature conserved β-barrel neck domains an...

Many spores from the Firmicutes group are decorated with hair-like structures. Since the 60s, they remained a mystery. @remaut-lab.bsky.social named these fibres ENdospore Appendages (ENAs). Using Cryo-ID, we report the identity, structure and function of F-ENA.
www.nature.com/articles/s41...

#MicroSky

Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis - Communications Biology Pseudomonas aeruginosa coordinates several virulence factors – the type 3 and H1-6 secretion systems and the flagellum – on a single cell level using the second messenger cyclic di-GMP, promoting subp...

Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis

@commsbio.nature.com from @diepold-lab.bsky.social at @mpimicrobiomarburg.bsky.social
with Victor Sourjik, Daniel Unterweger

www.nature.com/articles/s42...

#MicroSky

#MicroSky #AMR

#MicroSky

#MicroSky

Efficient mitochondrial A-to-G base editors for the generation of mitochondrial disease models - Nature Biotechnology Efficient adenine base editors for mitochondrial DNA are achieved with evolved TadA deaminases.

www.nature.com/articles/s41...

Programmable RNA acetylation with CRISPR–Cas13 - Nature Chemical Biology Yu et al. developed a programmable RNA acetylation system using dCas13–eNAT10 to specifically install N4-acetylcytidine on target RNAs in cells and in vivo, enabling functional studies of RNA acetylat...

www.nature.com/articles/s41...

Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology Deep proteome sequencing achieves ~80% coverage of the human proteome.

This paper advances deep proteome sequencing, improving detection of protein variants & isoforms beyond standard shotgun proteomics. By analyzing six human cell lines, it identifies a million unique peptides from 17,717 protein groups, highlighting translation of most alternatively spliced isoforms.

#MicroSky #AMR

Third track model for coordination of septal peptidoglycan synthesis and degradation by FtsN in Escherichia coli - Nature Microbiology Partitioning of FtsN and the septal peptidoglycan synthesis complex between an active synthesis track and an inactive denuded glycan track coordinates opposing peptidoglycan synthesis and degradation ...

Our new paper on FtsN is out! Now there are three tracks for organized cell wall constriction-- the old, boring E. coli always manages to surprise us.

A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.

www.nature.com/articles/s41...

Bacterial extracellular vesicles target different bacterial species, impairing cell division and diminishing their pathogenicity #microsky

#MicroSky

Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency - Nature Biotechnology Complete 5-methylcytidine substitution enhances the efficacy of self-amplifying RNA.

Complete 5-methylcytidine substitution enhances the efficacy of self-amplifying RNA go.nature.com/3RYbPHK
rdcu.be/em3ZT

#MicroSky

#MicroSky

Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.

How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z

Out in Nature today: A new immune signaling molecule, His-ADPR, is produced by defensive TIR domain proteins in bacteria to protect from phage

Joint work with the Tamulaitienė and Kranzusch labs

www.nature.com/articles/s41...

Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!

The bacteria that causes Q FEVER with some elegant NICHE ENGINEERING!

𝘊𝘰𝘹𝘪𝘦𝘭𝘭𝘢 𝘣𝘶𝘳𝘯𝘦𝘵𝘪𝘪 survives in a hostile PHAGOLYSOME by kicking out key host protease CATHEPSIN B

With Dot/Icm secretion and retrograde trafficking, it remodels its vacuole into a friendlier home

www.nature.com/articles/s41...

#MicroSky

#MicroSky

Treatment of acute myeloid leukemia models by targeting a cell surface RNA-binding protein - Nature Biotechnology An RNA-binding protein on leukemia cells provides an effective target in mouse models.

Check it out - amazing new work by @raflynn5.bsky.social and Kostas Tzelepis labs, discovery of a cell surface RNA/glycoRNA binding protein that is also a cancer antigen, new target for antibody therapy!

www.nature.com/articles/s41...

#MicroSky

A human arm and hand holding a bouquet of red carnations in front of a beige background.

Happy Freedom Day and 51st anniversary of the Carnation Revolution! 🇵🇹

Obrigado! 🌹

#MicroSky

#MicroSky

MMSB - Molecular Microbiology and Structural Biochemistry @mmsb-lyon.bsky.social is now on Bluesky!
👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...