Robin Rohwer

I'm on mac, but for me:

Cmd-F: find in console or script, whichever window cursor is in
Cmd-Opt-F: find in console, regardless of where cursor is
Cmd-Shift-F: find in files pop-up window
Opt-Shift-F: accented character Ï
Opt-F: mathy symbol ƒ
Cmd-Ctrl-F: full screen

I'm out of ideas to try..

keep getting tripped up that the updated @posit.co rstudio shortcut to run-the-current-function-definition has changed to find-in-console.

support page isn't updated (support.posit.co/hc/en-us/art...), has anyone else figured out the new keyboard shortcut? #rstats

Perhaps cumulative sum on the y-axis would show that despite a shift in timing we are actually back on trend line?

There is no vaccine for dengue you have definitely mixed up which childhood vaccines were cut

Wow this journal thinks their authors are so bad at writing that AI would do a better job

Half a century🤯 houses just have asphalt shingles around me (and water damage, if there's moss)

doesn't it damage the roof though??

Yeh and Fuhrman had several papers in 2019ish with an ocean mock that included at least thaumarchaea and they would've used v4-v5

Omg these are AMAZING 🤩😍🤩😍🤩😍🤩😍🤩😍🤩😍

Wow. It looks like it's growing on lake ice 😍

But you can just have AI do all that stuff now . ... 😬

A word cloud of my 2025 bluesky posts. Top words are 1. "diversity" 2. "strain" 3. "genomes" 4. "code" 5. "genes" and looks like "paper" is #6

A fun vanity app to see a word cloud of your 2025 posts: anisota.net/harvest

@bsky.app should make this a button to help you decide whether to follow back 😜

Not exactly, I went back and looked at when I recovered bins in my time series, and at the mapped reads to the dRep genome. Could see coverage and nucleotide diversity cutoffs below/above which I never recovered bins. 5-10x you may get bins, but start losing snps, thus my guess for subassembly

Being a parent did help me understand. I had to change peds to get my child their 2nd MMR before domestic travel near an outbreak (CDC only officially advises that for international travel). The disrespect when I asked for off-schedule (early) vaccination was shocking. No wonder parents lose trust

Really interesting discussion, especially your points about workflowing things. I do wonder though if some of it is a moot point because the sequencing tech itself changes so quickly

If they are highly abundant you could try a subassembly. By lowering an abundant organism to 5-10x coverage, you can lower its genomic diversity artificially via detection limit. I see both coverage and diversity cutoffs determine whether bins were recovered (if it works I'm curious to know!)

Didn't know this ASLO program included undergrads as well, what a cool opportunity for research abroad!

Minimap was faster than bbmap for me, but both worked fine on that scale of genomes. There is a setting with minimap you have to change to tell it to use more RAM though with big data

V4-EXT
www.biorxiv.org/content/10.1...

JEDI
www.biorxiv.org/content/10.1...

Now there's TWO recent preprints on 16S primer choice!

V4-EXT (V4 with broader diversity?)
JEDI (V4-V5 validated for 18S as well?)

Haven't wrapped my head around which is which and they're both brand new so no in-paper discussion

(ppr links below)

A friendship bracelet where the beads spell DR ROHWER

My friend knew this was a stressful week for me and dropped off this friendship bracelet 🤗😂 it is making me smile!

This is a compelling argument @froggleston.carpentries.org That blindly trusting the random person who wrote a package is not the same as blindly trusting an llm

This sentiment is not so different than wanting to understand generally what a package or library does, without worrying about the internal implementation.

Just noticed the Q&A comment boxes on peerJ articles now- wow! neat idea, curious if they'll be used

I dunno, yes you can dig for it, but does anyone actually? Like actually click through *all* old versions of a preprint to check for comments???

Biorxiv reviews just feel like reviewing into the void- public doesn't notice them, authors can ignore/bury them, now we learn even authors don't see it?

Missing from this discussion is that if authors want to bury a comment they don't like on their biorxiv preprint, all they have to do is just upload a new version. It happens even if they wanted it left in the public discussion, but want to address the comments with a new version!

I was discouraged from giving biorxiv reviews when I realized they are buried as soon as a new version is posted.

I think you should apply ;p After all it will fit "seamlessly into your existing commitments", and you've probably got an inside vote on the hiring committee

Pocket casts?

It is really cool that V4-V5 captures organelles so well... but I LOLed a little when I realized JEDI was just new branding for good old v4-v5 16S primers