LEK Lab

Making invisible excited state protein structures visible by combining NMR and machine learning NMR relaxation studies of the precursor form of the pro-inflammatory cytokine interleukin-18, pro-IL-18, show that it adopts two sparsely populated (<0.5%) and transiently formed (ms lifetimes) excite...

Jeff & Co. Just finished cooking this up. Give it a read!
👇👇👇👇
www.biorxiv.org/content/10.1...

The dynamic and heterogeneous composition of biomolecular condensates and its functional relevance Nature Reviews Molecular Cell Biology, Published online: 20 November 2025; doi:10.1038/s41580-025-00897-2Biomolecular condensates are non-membrane-encapsulated compartments that control various biological processes. Recent studies have revealed that condensates change in response to stimuli and over time. This Review discusses the heterogeneity and composition changes of nuclear and cytoplasmic condensates, their regulation and how the changes affect cellular biochemical reactions.

ICYMI: New Online! The dynamic and heterogeneous composition of biomolecular condensates and its functional relevance

The dynamic and heterogeneous composition of biomolecular condensates and its functional relevance Nature Reviews Molecular Cell Biology, Published online: 20 November 2025; doi:10.1038/s41580-025-00897-2Biomolecular condensates are non-membrane-encapsulated compartments that control various biological processes. Recent studies have revealed that condensates change in response to stimuli and over time. This Review discusses the heterogeneity and composition changes of nuclear and cytoplasmic condensates, their regulation and how the changes affect cellular biochemical reactions.

New Online! The dynamic and heterogeneous composition of biomolecular condensates and its functional relevance

Solution NMR goes big: Atomic resolution studies of protein components of molecular machines and phase-separated condensates The tools of structural biology have undergone remarkable advances in the past decade. These include new computational and experimental approaches tha…

Solution NMR goes big: Atomic resolution studies of protein components of molecular machines and phase-separated condensates, Alexander I.M. Sever, Rashik Ahmed, Philip Rößler, Lewis E. Kay* @lek-lab.bsky.social @utoronto.ca www.sciencedirect.com/science/arti... #NMRchat 🧲

Functional spectrum of USP7 pathogenic variants in Hao–Fountain syndrome: Insights into the enzyme’s activity, stability, and allosteric modulation | PNAS Hao&ndash;Fountain syndrome is a rare neurodevelopmental disorder caused by mutations in the deubiquitinating enzyme Ubiquitin-Specific Protease 7 (USP7)...

Our latest paper is online today ahead of print in PNAS
@pnas.org

www.pnas.org/doi/10.1073/...

The CSC and CSChE societies and communities express deep concern over proposed Tri-Council funding cuts. Canada must invest in science to ensure long-term health, innovation, and prosperity. Read the full statement here: buff.ly/peiCUfk

Application of NMR to Large RNAs Heteronuclear NMR methodologies developed over the past 40 years have enabled atomic level insights into the solution-state structure and dynamics of …

Journal of Molecular Biology (open) Review Article, Application of NMR to Large RNAs, Brian D. Grossman, Jan Marchant*, Michael F. Summers* www.sciencedirect.com/science/arti... #NMRchat 🧲

Dynamic interactions in the human β1AR signalling complex with mini-Gs revealed by NMR A wealth of insights into the mechanisms of GPCRs have been gained from biophysical studies of thermostabilized β1 adrenergic receptors (β1AR) from tu…

New paper from the Gossert lab: Dynamic interactions in the human β1AR signalling complex with mini-Gs revealed by NMR www.sciencedirect.com/science/arti...

Allosteric regulation of proteolytic machines unveiled by the synergy between cryo-EM and solution NMR spectroscopy pubmed.ncbi.nlm.nih.gov/40857152/ #cryoEM

BPS2026 Annual Meeting | Biophysical Society The BPS2026 Annual Meeting in San Francisco will showcase Biophysics by the Bay, highlighting the exciting advances in science and technology brought forth by big data and AI.  This year’s program…

Get important feedback from your community and move your work forward. Share your research at #bps2026!

Submit an abstract today buff.ly/oOjstk6

How a small lab @cicbiogune.bsky.social led by Óscar Millet, took on one of the world’s rarest diseases www.the-scientist.com/modeling-ang... #NMRnews #NMRchat #NMR 🧲

New paper alert! Stellar work by brilliant lead first author @annaleder.bsky.social together with project leader @masgu.bsky.social reveals a groundbreaking discovery: PDIA6 forms multi-chaperone condensates creating "folding factories" in the ER. www.nature.com/articles/s41...

Seedless: on-the-fly pulse calculation for NMR experiments - Nature Communications The authors develop a computational tool to design NMR pulses, based on the GRadient Assisted Pulse Engineering approach, that can calculate optimal pulse shapes on the fly during the measurements, to...

Nature Communications (open) Seedless: on-the-fly pulse calculation for NMR experiments, Charles J. Buchanan, Gaurav Bhole, Gogulan Karunanithy, Virginia Casablancas-Antràs, Adeline W. J. Poh, Benjamin G. Davis, Jonathan A. Jones* & Andrew J. Baldwin* www.nature.com/articles/s41... #NMRchat #NMR 🧲

#3: #PhD Position on Structure–Dynamics–Function Relationship of the #Chaperone #Hsp90:

➤ NMR spectroscopy for atomic-resolution
➤ Single-molecule FRET for time-resolved dynamics
Collab.: @hillerlab.bsky.social & Schmid lab @unibas.ch

More info & online application system: tinyurl.com/mr2em3nk

💥 We have 3 positions open in my lab in Basel, Switzerland🇨🇭:
#nanopores #smFRET #biomolecular #dynamics #singleMolecules

We’re looking for talented & ambitious new colleagues enthusiastic about biomolecular dynamics & single-molecule tech.

Please share broadly, thank you!🤝

schmid.chemie.unibas.ch

book cover

on behalf of Professor James Keeler (University of Cambridge) considering the possibility of revising the text and creating a third edition : Understanding NMR Spectroscopy, James Keeler - survey concerning a possible third edition cambridge.eu.qualtrics.com/jfe/form/SV_... #NMRbooks #NMRchat #NMR 🧲

Diffusing protein binders to intrinsically disordered proteins - Nature Using RFdiffusion, a general method for targeting intrinsically disordered proteins and regions for protein design has been developed.

Nature research paper: Diffusing protein binders to intrinsically disordered proteins

go.nature.com/4lSCdzE

Trump Administration Puts New Chokehold on Billions in Health-Research Funding The National Institutes of Health can’t award grants to outside researchers under a new White House restriction.

Absolutely outrageous interference in NIH extramural funding by OMB dropping this evening. Damaging to our nation’s world leadership, innovation, and training today and for years in the future. www.wsj.com/politics/pol...

#bioGUNE_Talent | 👏 Óscar Millet #CICbioGUNE @brtaeus.bsky.social, appointed Vice President of @group-ampere.bsky.social, Europe’s top #NMR organization, the first Spanish scientist to hold this role

🎉 Congratulations, Óscar!

🔗 ow.ly/hxi450Wuwiw

Great #NMR work on PTMs of histones by @roesslph.bsky.social and colleagues at @lek-lab.bsky.social!

Check it out!

I am (optimistically) looking for a postdoc with solution state biomolecular NMR expertise to join our group. I can provide access to high-end instrumentation and technical assistance via our membership at the New York Structural Biology Center. #nmr #academicsky #postdoc

Tenure track professorships

Tenure Track Professor of Structural Biology of Emerging Drug Targets at Medical University of Graz www.medunigraz.at/en/job-openi... #NMRjobs #NMRchat #NMR 🧲

The C-Terminal Tail of Ubiquitin-Specific Protease 7 Facilitates Ubiquitin Release and Ensures Efficient Catalytic Turnover USP7 is a key ubiquitin-specific protease involved in human development. Its activity is regulated by an intramolecular interaction between the cataly…

New publication alert!
Using NMR we show how the C-tail of USP7 contributes to its activation by facilitating ubiquitin release.

Online today in Journal of Molecular Biology.

www.sciencedirect.com/science/arti...

Good heavens. How are we supposed to do NMR when D2O prices are north of $1,500/L?

ADD YOUR NAME: Sign the Open Letter in Support of NIH Staff Join the courageous and committed National Institutes of Health (NIH) public servants by adding your name now.

Please join me and >5000 others in supporting NIH staff in their work supporting US biomedical research! actionnetwork.org/forms/add-na...

Group of scientists cutting red ribbon with large red scissors in front of a large 1.2 GHz NMR instrument.

Fun to celebrate inauguration of the National Gateway Ultrahigh Field NMR Center at Ohio State with the 1.2 GHz Bruker Ascend Spectrometer @osuchembiochem.bsky.social #nmr @brukercorporation.bsky.social

How a Key Enzyme Switches On and Off—And How a Rare Mutation Pushes It Into Overdrive USP7 is an important enzyme whose activity is tightly controlled in cells. Using advanced NMR techniques, researchers gained a rare glimpse into how the enzyme’s movements regulate its function. They ...

link.growkudos.com/1e2aa2yse0w

USP7 spotlight

Leveraging relaxation-optimized 1H–13CF correlations in 4-19F-phenylalanine as atomic beacons for probing structure and dynamics of large proteins - Nature Chemistry Correlating aromatic carbons attached to fluorine with meta-position hydrogens in fluorine-labelled phenylalanines can yield two-dimensional correlations with narrow linewidths in large proteins. Adap...

@natchem.nature.com Leveraging relaxation-optimized 1H–13CF correlations in 4-19F-phenylalanine as atomic beacons for probing structure and dynamics of large proteins www.nature.com/articles/s41... #NMRchat #NMR 🧲

This is no way to run a bagel shop or a country Academic science in the US is being gutted by Trump

NSF is in trouble. Here is my latest Substack post. Please share. open.substack.com/pub/artediso...