TJ Browne

This is seriously cool

Expression of Tacr1 and Gpr83 by spinal projection neurons Anterolateral system (ALS) projection neurons underlie perception of pain, itch and skin temperature. These cells are heterogeneous, and there have therefore been many attempts to define functional po...

New preprint from the Todd / Bell labs now on @biorxiv-neursci.bsky.social www.biorxiv.org/content/10.1...

Tour de force of central processing hubs from the gut. Awesome

Fantastic opportunity

What a year for the spinal cord connections lab!

Awesome news in the rundown of 2024.

Now for 2025!

An Open‐Source Tool for Investigation of Differential RNA Expression Between Spinal Cord Cells of Male and Female Mice Chronic pain is a highly debilitating condition that differs by type, prevalence, and severity between men and women. To uncover the molecular underpinnings of these differences, it is critical to an....

Interested in potential sex differences in gene expression in adult mouse spinal cord neurons? Excitatory versus inhibitory dorsal horn neurons? Check out this online tool and technical report that enables you to dig deeper. 👇😊

onlinelibrary.wiley.com/doi/10.1002/...

I expect big things on #fluorescencefriday, even the most banal cerebellum image is 🤯

🙋‍♂️ if I’m not in already. Struggling to find which I have already joined

Please?

Cheers Ewan

Cheers Simon!

It is a great shirt, found out of the need to hide potential grubby little finger marks hah. Though not sure it upstages Brett’s grin that day!

I have stolen our research programs post from the other place. We were fortunate enough to receive funding from ARC. Bring on the experiments #neuroskyence #painresearch @spinalcordguy.bsky.social

I forgot #fluorescencefriday patching away last night.

Spinal projection neurons in a sagittal section, amongst subP-IR terminals

So forgive me as just a naive end user of AAVs but with SIO taking up less room, larger Cre-dependent constructs can fit into the AAV?

🙋‍♂️ can I please be added

The migration has been made @spinalcordguy.bsky.social and @davehughesgla.bsky.social

The nervous system of a mouse imaged with tissue clearing and light sheet microscopy

Also contributing to #FluorescenceFriday

(also follow our lab account @erturklab.bsky.social for more stuff like this)

#FluorescenceFriday has been epic! Thanks for sharing!
I’ll put up a very boring characterisation of a transgenic line. Sagittal spinal section showing GC6 expression (green), inhibitory (blue/magenta)and excitatory neurons (red only)

Happy #FluorescenceFriday ! Let's celebrate with a colorful snapshot of the developing ventral spinal cord of a 🐣 embryo. #neuroscience #microscopy

Happy #FluorescenceFriday !
Let's celebrate with a colorful image of a developing ventral spinal cord of a 🐣 embryo.
#neuroscience #microscopy

You are a machine!

I appreciate you saying that, thanks.

Lateral Lamina V Projection Neurons labelled with Brainbow. #ArtorScience #neuroskyence
www.nature.com/articles/s41...

consequence of these neurons and the significance of collateral input at level and further afield, more work is to come #neuroscience #painscience #painresearch. Big thanks to the support of the Spinal Cord Connections lab at Uni Newcastle (Aus) and the bluesky-ve Brett Graham and David Hughes

were variable. This suggests that as LVlat projection neurons, which are assumed to be WDR and have large receptive fields following peripheral stimulation, are providing inputs back into the local spinal cord to engage various circuits, one of which is inhibition. As for the functional...

monosynaptic excitatory, polysynaptic excitatory and polysynaptic inhibitory components.
5. The monosynaptic neurons displayed functional ephys characters that are usually associated with inhibitory interneurons - high excitability (^APs), and low freq excitatory drive - while polysynaptic neurons..

the Dorsal horn.
4. Further evidence supporting this hypothesis was collected with optogenetics assisted circuit mapping, stimulating LVlat axons/terminals to identify postsynaptic neurons. Under these conditions ~16% of neurons sampled had a reliable optically evoked currents. There were...

cord, some remaining within the lateral white matter. They were also quite expansive in the rostrocaudal plane but had simple branches in all planes (phylogenetically old?)
3. Spinal photostimulation of these neurons led to activation patterns that suggested a local recruitment of neurons across ...

1. These neurons target the parabrachial nucleus. They occupy a discrete column within the lateral part of lamina V, bordering on the dorsolateral funiculus and could be quite selectively targeted using retroAAV constructs.
2. Their dendrites were biased towards the lateral and ventral spinal ...

Lateral lamina V projection neuron axon collaterals connect sensory processing across the dorsal horn of the mouse spinal cord - Scientific Reports Scientific Reports - Lateral lamina V projection neuron axon collaterals connect sensory processing across the dorsal horn of the mouse spinal cord

First post to bsky eep.. Work from my PhD was (finally) accepted: www.nature.com/articles/s41.... The main findings: as spinal projection neurons axons ascend to the brain they are influencing spinal circuits, adding a layer of complexity to their role and function locally. More details below...

🙋‍♂️ Please. Thanks for organising