Carballido-López's lab

The registration is now open for BACELL meeting on 8-9 June 2026 at University of Malaga, Spain.

Register and submit abstract for oral or poster presentation: bacell2026.eu

Deadline for abstract submission: 31 March 2026
Registration deadline: 15 May 2026

Organizer @diegromero.bsky.social

Let's get ready for a full month of training in image analysis in @pasteur.fr , this summer!

Are you interested in learning how to do bioimage analysis? For light microscopy? for cryo-EM? And for cellular cryo-EM?

Stay tuned, Pasteur offers access to THREE courses, this June.

👇🎓

Save the date for the next International Conference on Gram-Positive Bacteria which will take place between 29 June–2 July 2027. The organizers (@annadragos.bsky.social, @polonca32.bsky.social, and Ines Mandic-Mulec) look forward to welcoming you to Ljubljana, Slovenia

Convergent MurJ flippase inhibition by phage lysis proteins - Nature A common mechanism of inhibition of the essential lipid II flippase MurJ by three distinct phage-encoded single-gene lysis proteins provides insights into potential new targets for antimicrobial devel...

Convergent MurJ flippase inhibition by phage lysis proteins www.nature.com/articles/s41...

Checkout our latest preprint which is a real lab-wide effort to map antibiotic stress signatures across the pneumococcal pangenome. All findings have also been incorporated in PneumoBrowse 2.0.
www.biorxiv.org/content/10.6...

Structural mechanism and inhibitor discovery for DhhP, a Borrelia burgdorferi cyclic di-AMP phosphodiesterase with an Fe/Mn bimetallic center Klima et al. report the crystal structure of DhhP, a cyclic di-AMP phosphodiesterase of Borrelia. The structure reveals atomic details of the heterobimetallic active site and the overall asymmetry of ...

🫳 For c-di-AMP aficionados: Structural mechanism and inhibitor discovery for DhhP, a Borrelia burgdorferi cyclic di-AMP phosphodiesterase with an Fe/Mn bimetallic center!

In

👇

Structure www.cell.com/structure/fu...

@structure

Mechanistic basis of teichoic acid transport by a gatekeeper flippase www.biorxiv.org/content/10.6...

Lipid scrambling: New players, new questions, new opportunities Nearly a quarter of the proteins encoded in most organisms are transmembrane proteins. Contrary to textbook description, many feature a hydrophilic gr…

Amazing PhD @cristianrocharoa.bsky.social
and I have collected our thoughts and questions (many!) on lipid scramblases in this review:
www.sciencedirect.com/science/arti...
If you are interested in lipid transport and metabolism, membrane asymmetry and membrane protein function, give it a read!

The Origins of Agar First introduced into laboratories in 1881, agar remains indispensable as a culture medium.

The origins of agar
www.asimov.press/p/agar

We’re excited to announce the next Fluorescent Proteins & Biological Sensors conference! Abstract submissions are now open—hope to see you there.
www.janelia.org/you-janelia/...

Thank you for highlighting our paper on how septal PG hydrolysis licenses a second wave of PG transpeptidation during cell separation! www.nature.com/articles/s41.... I will do a thread when the page set version is out.

Our latest preprint: Together with the team of Jan Löwe, @danieltamarit.bsky.social and many others we discovered and characterized several Asgard tubulin genes and propose that microtubule architecture and dynamics evolved in Asgard archaea prior to eukaryogenesis
www.biorxiv.org/content/10.6...

SAVE THE DATE!
The next instalment of the long-running Bacterial and Archaeal Cell Division Conference will take place at www.marriott.com/en-us/hotels..., Italy from the 20th to 23rd of April 2027.
Organisers: me, @s-lab.bsky.social, @awehenkel.bsky.social.
More info soon.

#MicroSky

ATP-driven membrane binding and polymerization of bacterial actin MreB promotes local membrane fluidization https://www.biorxiv.org/content/10.64898/2026.02.13.705564v1

An SPFH Protein Couples Membrane Stress to Differentiation in Bacillus subtilis https://www.biorxiv.org/content/10.64898/2026.02.06.704340v1

The characteristics of autolysins associated with cell separation in Bacillus subtilis
-in #JBacteriology
journals.asm.org/doi/10.1128/...

New in JB: Jorgenson reviews recent advances in cell envelope assembly via the lens of the essential lipid carrier undecaprenyl phosphate and with an eye on targets of novel Abx development.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

Please share!!! I am looking for a postdoctoral researcher to join us. Focus will be on exciting collaborative projects on lipids and membrane biology, pushing the boundaries of in silico "reconstitutions". Previous experience with molecular dynamics simulations of biological systems a strong plus!

Identification of sporulation genes in Bacillus anthracis highlights similarities and significant differences with Bacillus subtilis How good is Bacillus subtilis as a model for the spore-forming pathogen Bacillus anthracis? Using high throughput genetics to identify B. anthracis sporulation genes and cytological analysis of the mu...

Fer’s tour de force in B. anthracis is out! Fer got Tn-seq running, built an ordered knockout library, defined all essential sporulation genes, and found a peptidoglycan deacetylase inhibitor critical for engulfment. Including our first one-by-all Alphafold screen! journals.plos.org/plosbiology/...

The Bacillus subtilis class A penicillin-binding protein 4 (PBP4) requires an accessory protein RpdA Peptidoglycan (PG) synthesis is essential to maintain cell integrity during bacterial growth and division. In Bacillus subtilis, PG synthesis involves class A PBPs that act independently and class B P...

The Bacillus subtilis class A penicillin-binding protein 4 (PBP4) requires an accessory protein RpdA www.biorxiv.org/content/10.6...

A decade later it’s still fun to see this in the wild

A teichoic acid-like wall modification associated with immune suppression is socially regulated in Streptococcus pyogenes https://www.biorxiv.org/content/10.64898/2026.01.07.698140v1

Quantitative Imaging: From Acquisition to Analysis Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)!

An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools.

🗓️ Apply online by Jan 30, 2026

RodZ acts through MreBCD to activate the elongasome in Escherichia coli https://www.biorxiv.org/content/10.64898/2026.01.05.697639v1

Wetting-Mediated Remodeling of the Cytoskeletal Cortex by Phase-Separated Condensates in Cell-Free Reactions Living cells dynamically coordinate gene expression with the spatial organization of their membranes and cytoskeletons. Unraveling how membrane-less organelles arise and interact with cytoskeletal net...

Wetting-Mediated Remodeling of the Cytoskeletal Cortex by Phase-Separated Condensates in Cell-Free Reactions
doi.org/10.1021/acs....

What is better than one? Two connected papers!

#NewResearch

S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.

#MicroSky 🦠

www.nature.com/articles/s41...

JB Editor's Choice: Anderson, Khursigara et al. review the role of peptidoglycomics in understanding the structure and function of peptidoglycan.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

wohoo! FtsZ aficionadas/os take note 👇
#MicroSky

Multi-omics profiling reveals atypical sugar utilization and a key membrane composition regulator in Streptococcus pneumoniae - Nature Communications The pathogen Streptococcus pneumoniae can adapt to diverse microenvironments in the human body. Here, De Bakker et al. study these adaptation responses, showing unusual sugar utilization and identifyi...

Really cool paper from @vincentdebakker.bsky.social @jonbakerlab.bsky.social now published @natcomms.nature.com #MicroSky www.nature.com/articles/s41...

Fluorescein-based dyes are not valid reporters of oxidative stress in bacteria, and conclusions based on their use must be reconsidered | PNAS Dihydrodichlorofluorescein derivatives have been employed as reporters of intracellular reactive oxygen species (ROS) in innumerable studies. Their...

www.pnas.org/doi/10.1073/...