Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress Cryo–electron tomography reveals how mitochondria reorganize their translation and folding systems under proteostatic stress.

Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress | Science Advances www.science.org/doi/10.1126/...

Function and regulation of the mitochondrial stress response - Nature Structural & Molecular Biology In this review, the authors discuss and contextualize the cellular responses launched after mitochondrial stress and note the importance of the emerging understanding in applying this new knowledge in...

March kickstarted @natsmb.nature.com focus on Proteostasis. Throughout the year we will be publishing state-of-the-art Reviews and Perspectives. What a better way to start than excellent Review by Michael Rapé and colleagues on the mitochondrial stress response. www.nature.com/articles/s41...

New preprint from
@novoalab.bsky.social !

Which tRNAs are used by ribosomes during translation?

We introduce tRIBO-seq, a nanopore method to sequence ribosome-associated tRNAs and track how the active tRNA pool changes across stress conditions.

www.biorxiv.org/content/10.6...

Thread 👇

A conserved archaeal ribosome-associated factor linking bacterial hibernation and eukaryotic energy sensing https://www.biorxiv.org/content/10.64898/2026.02.22.707292v1

Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes Cells down-regulate protein synthesis when stressed to conserve energy and shift resources toward repair. We found that in some mammalian cells, including neurons, stress also resulted in the formatio...

Out now in @science.org
Ribosomal RNA expansion segments mediate the oligomerization of inactive animal ribosomes | Science www.science.org/doi/10.1126/...

Bacterial GTPases act as successive placeholders to mediate ribosome assembly and its coupling to translation initiation www.biorxiv.org/content/10.64898/2026.02.15.706057v1 #cryoEM

Please spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳

mrc.tal.net/vx/mobile-0/...

Ribosome biogenesis and homeostasis: in the front line to cope with cellular stress Cells must continuously adapt to both internal and environmental stresses by finely tuning their molecular and metabolic activities. One of the most r…

Fascinated by how cells adapt to a plethora of stimuli?

​This review describes how cells use and modulate #ribosomes to cope with stresses. We discuss the central role of ribosome-associated processes in #cellplasticity, #signaling, #geneexpression and #diseases 🕹

@cbitoulouse.bsky.social

Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In Arabidopsis, the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SU...

In @science.org this week, thermospermine affects ribosome methylation, which in turn regulates translation of xylem development proteins www.science.org/doi/10.1126/...

#plantscience

Blocking RAN translation without altering repeat RNAs rescues C9ORF72-related ALS and FTD phenotypes GGGGCC (G4C2) repeat expansion in C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Toxicity is thought to result from the accumulation...

I'm thrilled to anounce that our latest study on ALS/FTD neurodegenerative diseases has been published. Very proud to be part of this excting project. Many thanks to Clotilde Lagier Tourenne for an amazing longlasting and fruitfull collaboration, #ALS #FTD #Ribosomes
www.science.org/doi/10.1126/...

Signaling to make human ribosomes: Connections between the cytoplasm and the nucleolus Lawrence et al. examine how ribosome production in the eukaryotic nucleolus is coordinated by cytoplasmic processes to maintain appropriate ribosome numbers. The authors highlight regulatory inputs from cytoplasmic signaling, mitosis, and cytoplasmic organelles that converge on nucleolar function, and they discuss how disruption of these pathways contributes to human disease.

Online Now: Signaling to make human ribosomes: Connections between the cytoplasm and the nucleolus Online now:

Postdoctoral Researcher - Structural Studies - Dr Suyang Zhang - LMB 2761 - Cambridgeshire (GB) job with MRC Laboratory of Molecular Biology (LMB) | 12852642 Postdoctoral Researcher Salary £42,694 per annum  MRC Laboratory of Molecular Biology, Cambridge, UK We are recruiting a Postdoctoral Researcher to...

🚨Postdoc opportunity🚨Our lab at MRC-LMB Cambridge is recruting a postdoc to study transcription-coupled splicing using cryoEM and endogenous isolation! 🔬 🧬

Please feel free to get in touch directly #postdoc #cryoEM #biochemistry #splicing

Apply here by 11 Feb
www.nature.com/naturecareer...

When RNA Degradation 🤝 meets 🤝 Protein Degradation! tinyurl.com/E3TDMD In a collaboration of @bartellab.bsky.social and Schulman lab, we show that, in target-directed microRNA degradation (TDMD), 2-RNA-factors recruit an E3 ligase and induce the degradation of not only a protein but also RNA (1/5).

HYPK promotes N-terminal protein acetylation through rapid ribosome exchange of NatA Huntingtin-interacting protein K (HYPK) promotes the activity of N-terminal acetyltransferase A (NatA), which cotranslationally acetylates 40% of the eukaryotic proteome. Kinetic and in-cell measurements revealed that HYPK acts as a ribosome exchange factor for NatA, enabling its access to and acetylation of the translatome.

HYPK promotes N-terminal protein acetylation through rapid ribosome exchange of NatA

Mechanism of cotranslational modification of histones H2A and H4 by MetAP1 and NatD NAC enables cotranslational N-terminal processing of histones H2A and H4 by recruiting MetAP1 and NatD at the ribosomal tunnel.

Last X-Mas, the ribosome gave you methionine,
but the very next day, MetAP took it away.
This year, to save histones from tears,
NatD gives you an acetyl group. ⭐️

Explore our latest paper with the Deuerling lab @uni-konstanz.de and Shu-ou Chan lab @caltech.edu!

www.science.org/doi/10.1126/...

Our latest paper on the molecular mechanisms of XPF-ERCC1 recruitment to SLX4-dependent DNA repair pathways has been published! Congratulations to first author Junjie Feng, and a heartfelt thank you to all our collaborators for their contributions to this study!
www.nature.com/articles/s41...

Multiscale structure of chromatin condensates explains phase separation and material properties The structure and interaction networks of molecules within biomolecular condensates are poorly understood. Using cryo–electron tomography and molecular dynamics simulations, we elucidated the structur...

@science.org 🧬🔬 Multiscale structure of #chromatin condensates explains phase separation and material properties | Science www.science.org/doi/10.1126/... @janhuemar.bsky.social et al.

GTPBP1 is a GTPase implicated in neurodevelopment. Our cryo-EM maps show GTPBP1 delivering tRNA to ribosome.

Due to delayed GTPase dissociation, mRNA decoding is more stringent than with bona fide delivery factor eEF1A.

Congrats to Denis Susorov and collab with Pestova lab! tinyurl.com/3smdvt3v

Using "in extracto cryo-EM", we visualize ribosomes in mammalian lysates, including RRL. "Hibernating" ribosomes carry an extended set of proteins that protect functional centers. These include elongation factor eEF2, LARP1 implicated in mTOR signaling, eIF5A etc. www.biorxiv.org/content/10.1...

ZAK activation at the collided ribosome - Nature The kinase ZAK is activated at collided ribosomes to mediate the ribotoxic stress response.

Check out our latest work on how collided ribosomes activate the MAP3K ZAK! 💫

www.nature.com/articles/s41...

A fun collaboration with @beckmannlab.bsky.social @doubleshuang.bsky.social

Ribosome remodeling drives translation adaptation during viral infection and cellular stress https://www.biorxiv.org/content/10.1101/2025.10.24.684008v1

Oxidative stress sensing by the translation elongation machinery promotes production of detoxifying selenoproteins Selenocysteine, incorporated into polypeptides at recoded termination codons, plays an essential role in redox biology. Using GPX1 and GPX4, selenoenzymes that mitigate oxidative stress, as reporters,...

Colliding ribosomes are potent signals of cellular stress. But do cells use ‘programmed’ ribosome collisions to regulate gene expression? I’m excited to present a new story from my lab led by Frederick Rehfeld(@fred-rehfeld.bsky.social) which revealed that the answer is YES! Read on to find out how👇

Two microbiome metabolites compete for tRNA modification to impact mammalian cell proliferation and translation quality control - Nature Cell Biology Zhang, Lahry, Cipurko et al. show that the microbial metabolites queuine and preQ1 modify the same host tRNA. PreQ1-tRNA reduces cell proliferation, slows tumour growth, decreases the translation of ribosomal proteins and is cleaved by IRE1 on the ER ribosome.

☕Pan, David, Chevrier & co show that the microbial #metabolites queuine and preQ1 modify the same host tRNA. PreQ1-tRNA reduces cell proliferation, slows #tumor growth, decreases the translation of ribosomal proteins and is cleaved by IRE1 on the ER #ribosome.
👉https://rdcu.be/eJqFc
bit.ly/46BPXtJ

Nothing like a coffee break to fuel great conversations ☕✨ Our #EMBLProtein participants are connecting and recharging after the first half of session 1 'Ribosome, elongation, and termination'.

Starting Session 2 'Quality control' strong!

🎙️Oliver Mühlemann opens with 'Deciphering the role of the deubiquitinase USP9X in ribosome-associated quality control' at #EMBLProtein.

We’re back! 🥳

Kicking off our first conference after the summer break: 'Protein synthesis and translational control'.

🌟Today’s highlights include keynote lectures from Lori Passmore, Reuven Agami, and Marina Rodnina.

Welcome to #EMBLProtein! 🎉

Finally out!
I’m thrilled to share our new paper (Wolin et al., Cell 2025).

This paper describes SPIDR, a high-throughput method for mapping RBP binding sites.

By combining #SPIDR with #cryoEM, we identified the exact binding site of LARP1 within the #mRNA channel of the 40S ribosomal subunit.

m6A-induced ribosome stalling and collision trigger mRNA decay How m6A in different transcript regions regulates mRNA decay remains an intriguing question. Three studies from the labs of König, Jaffrey, and Steinmetz uncovered translation-dependent CDS-m6A decay: m6A in the coding sequence (CDS) induces ribosome stalling and collision to trigger mRNA decay, while tRNA modification alleviates the effects.

m6A-induced ribosome stalling and collision trigger mRNA decay

📣 Online now - the Review "The ribosome ubiquitination code: fine-tuning translation under stress" from
Haleigh Wooters, Neil Nimmagadda, Alicia Darnell, and @thesilvalab.bsky.social .

#Ubiquitination #TranslationalReprogramming #StressResponse

Read it here: authors.elsevier.com/a/1lPPS3S6Gf...

New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome!
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...