Salaita Lab

🔬 #Mifobio2025

@salaita.bsky.social's lecture is online !

🎤 “May the Force Be Measured : mechano probes reveal the molecular forces generated by cells”

👉 youtu.be/H853ftTAaM4

@emoryott.bsky.social @salaitalab.bsky.social @cnrs.fr @cnrsingenierie.bsky.social @cnrsbiologie.bsky.social

#GDRimabio

Mechano-ID: Proximity Labeling of Mechanically Active Receptors Reveals the Mechanome and Tags Mechanically Active Cells A major challenge in the field of mechanobiology relates to the lack of methods that enable the identification of mechanically active receptors, associated proteins, and the individual cells that display enhanced force generation. For example, potent T cell activation requires the transmission of biophysical forces between the T cell receptor (TCR) and its peptide-loaded major histocompatibility (pMHC) complex antigens. Interestingly, TCR-antigen interactions are highly dynamic, displaying a broad range of force magnitudes between different cells and even within the same cell. Therefore, methods that can chemically tag mechanically active T cells, TCRs, and their associated proteomes, or mechanomes are highly desirable. Such techniques may enable a deeper understanding of the mechanisms governing immune responses and may also have broad applications in immunotherapy. Herein, we report a technique dubbed mechano-ID, which allows for mechanically selective proximity tagging by leveraging DNA-based molecular force probes that recruit proximity tagging enzymes. We demonstrate mechano-ID tagging of T cells using microscopy and flow cytometry, with further confirmation by proteomics and Western blotting of mechanically active T cell receptors.

Ever wonder which #Tcell receptors are actually pulling their weight? Our lab's new #JACS paper, Mechano-ID, uses proximity labeling to uncover receptors that engage under mechanical force 😎

Huge congrats to co-authors Rong, Hus, and the team for making this possible!

#emory #chemistry #immunology

#Mifobio2025

Focus on the module 5:
👉 Physical measurements, control and handling -Mechanobiology

We are delighted to welcome Professor @salaita.bsky.social @salaitalab.bsky.social

Lecture - “May the Force Be Measured : mechano probes reveal the molecular forces generated by cell

#GDRimabio

DNA Origami Tension Sensors (DOTS) for Single-Molecule Force Measurements at Fluid Intermembrane Junctions A key event in triggering adaptive immunity is the binding of a T cell receptor (TCR) to its antigen at the T cell–target cell interface. Mechanical forces are critical for TCR–antigen interactions, w...

It's an awesome Monday for the Salaita lab! Our latest paper was just accepted to #ACS #NanoLetters. Huge congratulations to Sarah Al Abdullatif, Alexander K. Foote Yuesong Hu, and Jhordan Rogers for their incredible work and well-deserved recognition 🧠💪

#emory #chemistry #DNA #tensionsensor #tcell

Congratulations to Joshua on receiving the BDCI T32 Predoctoral Training Fellowship! This well-deserved recognition reflects both his hard work and incredible potential. We’re so proud and excited to see where his research takes him!

#research #PhD #emory #chemistry

Science by day, art by night 🧑‍🎨 Our lab's annual paint night was a great way to unwind together. Always grateful for #science, #community, and a good splash of creativity!

#emory #chemistry

We successfully held our first annual workshop, Mechanobiology Methods: From Molecules to Cells (M3C), last week. We hosted 16 graduate students and postdocs from across the country for lectures and labs. So many wonderful and inspiring conversations with the next generation of researchers!

On April 25, our dept held its annual Awards Ceremony. Big congrats to Rachel for receiving the Quayle 4th-Year Excellence in #Research #Award and to Maia for the Citizen #Scholar Award!
We're so proud of you both and of all the amazing awardees who continue to uplift and inspire our community!

HCS is pleased to announce our travel award winners!

These awardees will participate in the upcoming Analytical and Quantitative Light Microscopy (AQLM) workshop next month at Marine Biological Laboratory.

Congratulations to Jessica, Anna, Amir, Erin, and Aliyah!

Excited to share highlights from the awesome time our lab had at the #AtlantaScienceFestival! It was a blast showing folks of all ages how DNA is more than just genetic material but can be used as a tool to understand the world around us! 🧬💪😎
#DNA #mechanobiology #biophysics #chemistry #emory #atl