Excited to share our latest research, out today in Cell (@cellpress.bsky.social)
Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling.
Read the paper here: doi.org/10.1016/j.ce...
When RNA Degradation π€ meets π€ Protein Degradation! tinyurl.com/E3TDMD In a collaboration of @bartellab.bsky.social and Schulman lab, we show that, in target-directed microRNA degradation (TDMD), 2-RNA-factors recruit an E3 ligase and induce the degradation of not only a protein but also RNA (1/5).
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
New paper alert! Scientists in Clemens Plaschkaβs lab at the IMP and @juliusbrennecke.bsky.social's lab at
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cellβs nucleus to its periphery. More: bit.ly/4nHcvys
Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...
Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social
www.cell.com/cell/fulltex...
Working on Death Domain Fold proteins (CARD, Pyrin, DED, DD)? Check out our new paper for some more perspective:
www.pnas.org/doi/10.1073/pnas.2426986122
Happy to share our new study showing that the Ub E3 ligase SIAH1, known to dimerise via its C-term SBD, also dimerises via its N-term RING. When these tendencies combine, full-length SIAH1 forms multimers, which might explain its clustering in cells & preference for aggregated/multimeric substrates.
Best way to start 2025! Thrilled to share our Nature paper about the structures of axonemal components from sperm flagella and from epithelial cilia of the oviduct and brain ventricles. Great collaboration with Tzviya Zeev-Ben-Mordehai and @alanbrownhms.bsky.social
www.nature.com/articles/s41...
Architecture of the 1.3 MDa complex of human UBR4 (2 x UBR4/KCMF1/CALM1). Picture shows top and side views of the cryo-EM density map and the modeled structure, colored by protein components and domains.
π xmas preprint π we are excited to share our cryo-EM structure of UBR4 in complex with KCMF1 and CALM1. the PQC ligase forms a massive ubiquitination arena, primed to amplify ubiquitin chains (E4 activity) and boost degradation of defective proteins. www.biorxiv.org/content/10.1...
giant e3 ligases shaking in their boots as Dan crosses another one off the list
Are you a #postdoc with an innovative vision for starting your own lab in #Syntheticbiology, #Genomics, #GeneRegulation, #Development, #Cancer, #Immunology or #InfectionBiology?
@IMPvienna is inviting applications for group leader positions: www.imp.ac.at/career/open-...
Pls share!
We are still considering postdoc candidates for the post advertised below. A 2-year contract beginning in 2025 to work on protein SUMO E3 ligases and the nucleosome.
Check out this list of ubiquitin and Ubl profiles to follow. Repost if you want me to add you to it. Letβs create a good community here βοΈ go.bsky.app/3VPQofn
Hard to convey how positively working with Marcin will impact your science- can not recommend enough.
