Luiz Passalacqua

RNA: Searching for druggable targets A strategy that analyzes the structural properties of RNA could help identify regions that are promising targets for antiviral drugs.

An editorial on our work publsihed in @elife.bsky.social
@ucllifesciences.bsky.social #GQuadruplex

Amazing! Looking forward to the upcoming papers!

Structural switching dynamically controls the doubly pseudoknotted Rous sarcoma virus–programmed ribosomal frameshifting element | PNAS A hallmark of retrovirus replication is the translation of two different polyproteins from one RNA through programmed –1 frameshifting. This is a m...

Excited to share my latest research. In this paper in PNAS, I use #cryoEM and #crystallography to solve multiple new structures of a viral RNA motif that controls frameshifting.

#virus #retrovirus #structuralbiology #science #biochemistry

www.pnas.org/doi/10.1073/...

Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display - Nature Communications Understanding how RNA folds into functional structures requires methods that can systematically assess how sequence perturbations affect RNA structure and function. Here, the authors develop a broadly...

Our paper describing our Transcription Elongation Complex Display platform for high-throughput cotranscriptional RNA functional assays is now online at @naturecomms.bsky.social: www.nature.com/articles/s41.... We used TECdisplay to assess the activity of >1M ZTP riboswitch variants!

Amazing! Congratulations!

Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

Our latest preprint, led by Skyler Kelly: we developed a platform for high-throughput cotranscriptional RNA biochemistry called Transcription Elongation Complex display. www.biorxiv.org/content/10.1...

Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing ... RNA begins to fold as the nascent transcript emerges from a transcribing RNA polymerase. Here, the authors develop a concise method for mapping RNA folding pathways that couples in vitro transcription...

The final version of our article describing a modernized procedure for cotranscriptional RNA structure probing is online! www.nature.com/articles/s41...