Abhi Aggarwal

Glutamate is the primary molecule that neurons use to communicate with each other.

Previously, scientists have mostly recorded when neurons fire output signals, but now with a new glutamate indicator, they can record the many inputs that causes cells to fire. 🧵

#neuroskyence

Glutamate indicators with increased sensitivity and tailored deactivation rates - Nature Methods iGluSnFR4f and iGluSnFR4s are the latest generation of genetically encoded glutamate sensors. They are advantageous for detecting rapid dynamics and large population activity, respectively, as demonst...

iGluSnFR4 is now available @natmethods.nature.com

www.nature.com/articles/s41...

Glutamate indicators with increased sensitivity and tailored deactivation rates 🧪

We haven’t observed any clear signs of cytotoxicity or aggregation, in contrast to what we’ve occasionally seen with jRGECO1a. We imaged at around 2 weeks in cultured cells and 4–6 weeks in vivo.

A sensitive orange fluorescent calcium ion indicator for imaging neural activity Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance...

#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪

A sensitive orange fluorescent calcium ion indicator for imaging neural activity

www.biorxiv.org/content/10.1...

Where can I get reagents?

Bacterial (pRSET) and mammalian (pAAV) expression vectors, including different promoters, Cre- and Flp- dependent vectors, and both the NGR and PDGFR membrane targeting sequences, are available from @addgene.bsky.social. /3

www.addgene.org/browse/artic...

🚨iGluSnFR4 is finally out!🚨🧪

We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️

www.biorxiv.org/content/10.1...

🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX

#Neuroscience

Hi Dani! Can you add me whenever you get a chance?
scholar.google.com/citations?us...

Thanks for organizing, can you add me?
scholar.google.com/citations?us...

Hi, thanks for organizing. Can you add me?
scholar.google.com/citations?us...

High-speed video showing glutamate lighting up as it’s released in synapses, visualizing the neurotransmitters of the brain with the fluorescent indicator protein iGluSnFR3.

Credit: Allen Institute, Aggarwal et al.

We’re hoping to have the preprint out within the next month or two🤞

First sky post 🦋 Sharing some vibrant iGluSnFR3 minis in cultured neurons!

I’d love to be added! 😊