Our study does not address the PL/LPS biosynthesis/transport coordination. However, talking about coordination, we speculate that the lipid tails of LptM help prevent that PLs of the OM inner leaflet access the lateral gate of LptD when this opens to release LPS.
A special thank you also to the METI platform @cbitoulouse.bsky.social
Indeed, fantastic collaboration!
rdcu.be/eRxNf here our new study on the characterization of the lipopolysaccharide holotranslocon, LptDE and the newly identified subunits LptM and LptY. Great collaboration with @fronzeslab.bsky.social @pstansfeld.bsky.social @JulienMarcoux @YvesQuentin
The small lipoprotein LptM intercalates the LptD lateral gate promoting gate dynamics and access by LPS. LptY (YedD) stabilizes the beta-taco domain and contributes together with LptM to optimal functioning of the Lpt transenvelope bridge.
The LptD beta-strand 1, defining the mobile side of the lateral gate, binds LPS and performs a stroke movement toward the external leaflet during the contracted-to-extended state transition.
In this paper we show that LptD functions via a conformational switch of the beta-taco/beta-barrel interface, which alternates between contracted and extended states.
Here our new preprint on the LPS holo-translocon doi.org/10.1101/2025... , collaboration with Yves Quentin @JulieMarcoux @fronzeslab.bsky.social @pstansfeld.bsky.social - @cbitoulouse.bsky.social
Spoiler of our new preprint: How does LPS cross the bacterial outer membrane?
Last days to send your application! deadline December 20!
Let's start the Bsky adventure with a job post. Still about two weeks to apply to this postdoc position on "Molecular and Structural Biology of Membrane Biogenesis" in the labs @raffaeleieva.bsky.social ky.social and @fronzeslab.bsky.social slab.bsky.social in beautiful south-west of France!
