Jacob Smith

Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues - Nature Communications Here proteomic chromatome analysis shows metabolic enzymes widely localize to chromatin in cancer in a tissue-specific manner. Nuclear enzymes affect DNA damage/repair and transcription, revealing non...

New paper out in Nature Communications:
www.nature.com/articles/s41...
We found >250 metabolic enzymes on chromatin.
Only ~20 had been reported before.
This means hundreds of metabolic enzymes may have unexplored nuclear roles.

for free access, see: diposit.ub.edu/items/3d97e3...

This graphical abstract summarizes the role of the liver circadian clock in regulating skeletal muscle metabolism through endocrine signaling. In wild-type (WT) mice, the liver clock is intact (functional), whereas in hepatocyte-specific Bmal1 knockout mice (Bmal1hep−/−) the liver clock is disrupted (core clock rhythms completely absent). RNA-sequencing analysis of skeletal muscle shows that deletion of the liver clock does not alter the core molecular clock machinery in muscle but modifies the rhythmic expression of metabolic genes, particularly those associated with mitochondrial and lipid metabolism. To investigate whether this regulation occurs through circulating factors, serum collected from WT or Bmal1hep−/− mice during different circadian phases was applied to cultured skeletal muscle myotubes. RNA-sequencing of myotubes revealed that serum from Bmal1hep−/− mice, particularly during the dark phase (ZT16), reduces the expression of oxidative phosphorylation and other mitochondrial genes compared with WT serum. Functional assessment demonstrated that treatment with Bmal1hep−/− serum decreases mitochondrial respiration and ATP production in myotubes. Together, these findings illustrate that the liver circadian clock communicates with skeletal muscle through blood-borne signals to fine-tune muscle metabolic gene expression and mitochondrial function without directly altering the intrinsic muscle clock. This endocrine liver–muscle signaling axis highlights a mechanism by which peripheral clocks coordinate systemic metabolism across tissues.

🧪 Very happy to have our story out in Journal of Biological Rhythms! In collaboration with Lab of Kevin Koronowski (UTHSA), we show that the liver clock regulates 24h transcriptional rhythms in skeletal muscle via serum-based signalling. pubmed.ncbi.nlm.nih.gov/41486525/. Thanks to all authors! 🙏

Peer Review is broken because a generation of Editors were trained that peer review is sacrosanct. Thus we have Editors who are clerks, sending and re-sending manuscripts to reviewers until they are happy. That's not the job. Be an Editor, not a clerk. Use your skill and judgement. Make decisions.

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects - Nature Communications The authors uncover a mechano-metabolic adaptation where confinement induces rapid mitochondrial relocalization to the nuclear periphery, generating localized nuclear ATP surges that support chromatin...

🚀 We’re excited to share our latest publication in collaboration with Verena Ruprecht's group at @crg.eu

Mechanobiology meets Nuclear Metabolism

www.nature.com/articles/s41...

🥳🎉 Congrats @ritoghose.bsky.social and @sdelcilab.bsky.social Beautiful data!!

Functional liver genomics identifies hepatokines promoting wasting in cancer cachexia Cancer-induced liver changes promote body wasting through secreted factors, with circadian regulators and hepatokines being important contributors to cancer cachexia.

🧪 Really cool new #circadian paper looking at the role of the liver clock in inter-organ crosstalk with muscle during Cachexia "Functional liver genomics identifies hepatokines promoting wasting in cancer cachexia": Cell www.cell.com/cell/fulltex...

Part time (20h per week) technician/predoc position available in my lab at the University of Barcelona 🧬⏰ studying circadian rhythms & inter-organ crosstalk.

Starts 1st Sept 2025.

Contact via email link in website (sites.google.com/view/smithla...) for more info

#ScienceJobs #Barcelona #Circadian

Yeh a lot do for sure- its really sad- about 50% voted for him at least (49.9% of popular vote was for Trump in 2024). Reminds me of the percentages for Brexit in UK (another terrible and embarrasing decision), 52% for, 48% against. Signs of deeply divided populations...

🧪 Encouraging to see people standing up against tyranny and fascism in the US. The Trump administration’s anti-science and pro-war agenda isn’t just dangerous at home — it threatens progress and stability worldwide. Enough!

US science is being wrecked, and its leadership is fighting the last war Facing an extreme budget, the National Academies hosted an event that ignored it.

The failure of the National Academy of Sciences USA to make a clear and unequivocal statement about the disastrous Trump administration science policies — it lets all of us down and dooms them to eventual irrelevance.

US science folks: it's been really heartening to see all the Stand Up For Science posts. Thank you for showing the backbone and resolve that most institutions have lacked.

Great to see US scientists protesting in response to the insanity that is the US "government" right now. @standupforscience.bsky.social 🧪

New preprint from the lab! 🚨

🧫 New galactose #CRISPR screening strategy
🎯 481 genes required for #OXPHOS
⚙️ #FAM136A in IMS proteostasis
👂Mito defects in #Menieres disease

by @marcellharhai.bsky.social @fbm-unil.bsky.social

A short thread 1/5

www.biorxiv.org/content/10.1...

Congrats and many thanks for sharing, this is really informative!

Thanks Pablo! 🙏

Hi, you can check my post for the free SharedIt link. If it doesn't work let me know

Check my profile for the sharedit link, thanks

As we age, our circadian clock goes off-track, which may contribute to age-related diseases. A new review paper gets into the mechanisms and potential ways to prevent the decline www.nature.com/articles/s41...

Circadian clock communication during homeostasis and ageing Nature Reviews Molecular Cell Biology - Communication between the circadian clocks of different cell and tissue types supports the daily rhythms of homeostatic processes that maintain bodily...

🧪 ✍️ Please check out our new review on intercellular and interorgan communication between circadian clocks ⏰🧬 out today in Nature Reviews Molecular Cell Biology 🎉
@natrevmcb.bsky.social
! Here is the SharedIt link for free access: rdcu.be/d5ppE

Congrats to you, your team and collaborators on a series of amazing manuscripts! 👏🤯

Wow, this is quite wild!

Hi Pancho, can I be added please? 🙏

It seems easy to make fun of basic science – for example why would you want to study bacterial immune systems? But then you would have to admit that that’s exactly what led to the discovery of using CRISPR as an insanely good technology for genome engineering.

Hi Christian, could you please add me to this? 🙏 all the best

Wow, this is cool. Non natural cis elements designed in silico that are more effective than natural

If you just moved to BlueSky and interested in #chronobiology, find some of your community here!

Want to be added? Reply with evidence your work relates to chronobiology.

go.bsky.app/Sw8L4aS

With all the new people coming over, I’ll repost weekly for the next month or so.

🧪 🌍 🦑 #EvoBio

Liver and muscle circadian clocks cooperate to support glucose tolerance in mice Smith et al. rescued circadian clock function in liver and muscle in otherwise clock-less mice, finding that glucose tolerance is achieved only when feeding rhythms and Bmal1 function in liver and mus...

Hi Nicholas 👋, could you add me please 🙏🕙💪 www.cell.com/cell-reports...

This month we published 2 MANUSCRIPTS in @natureportfolio.bsky.social , nature communication
1. Natalia Pardo discovers the role of nuclear MTHFD2 in centromere stability
2. Espinar and Garcia-Cao reveal the role of nuclear IMPDH2 in the DNA damage response.

Nuclear Metabolism is on 🔥