Ada Muñoz

Equipazo!✨👑

Glad to see our latest work out in Nature Microbiology!!

Extremely grateful to everyone involved in the project.

Check it out!! 👇🏻👇🏻

Check out the latest version of our paper — peer-reviewed, polished, and published. Hope you like it!

www.nature.com/articles/s41...

Plasmids promote antimicrobial resistance through insertion sequence-mediated gene inactivation - Nature Microbiology Inactivation of chromosomal genes through plasmid-encoded IS elements is an extended mechanism of antimicrobial resistance evolution in bacteria.

Final version of our last paper is out!
www.nature.com/articles/s41...

Excited to share our latest work! 📝

We measured the fitness effect of 136 AMR genes and found that many are neutral or even beneficial without selection. 🤯🧬

Oxygen availability can flip their fitness and our stochastic model indicates that oxygen fluctuations help maintain them.

Learn more 👇🏼

New preprint out on bioRxiv!

www.biorxiv.org/content/10.6...

Can conjugative plasmids be used to control plasmid and pathogen spread?

Follow me down the rabbit hole that led to this story 🧵

What a fantastic new addition to my lab! 🚀

Welcome @albertohca.bsky.social to the @uam.es family! Stay tuned for the science we’re going to build together in the coming years!

A short research stay in 2019 (back in the pre-COVID era) at José R. Penadés lab turned into the third chapter of my PhD and today it finally sees the light.

And what a beautiful @pnas.org cover @paularamiro.bsky.social and @jerorb.bsky.social !!!!!! You should be very proud!

Plasmid mutation rates scale with copy number | PNAS Plasmids are extrachromosomal DNA molecules that spread by horizontal transfer and shape bacterial evolution. Plasmids are typically present at mul...

New paper out in PNAS!!! 🎉

Do more plasmid copies mean faster evolution?

🧵 Dive into the story

www.pnas.org/doi/10.1073/...

Secret Invasion: Strain Fate Across Microbiomes

New #BehindThePaper for our latest paper in @natcomms.nature.com (rdcu.be/e2qMK)
Secret Invasion: Strain Fate Across Microbiomes

Cover illustration by Helena Klein (@illuzation.bsky.social).
communities.springernature.com/posts/secret...

Frontiers | Genome assembly and functional predation analysis of novel Bdellovibrio isolates from human gut microbiota IntroductionPredatory bacteria of the Bdellovibrio and like organisms (BALOs) have long been postulated as living antimicrobials, yet their occurrence and ec...

🧬New paper out! We report the first isolation of viable B. bacteriovorus predators from human gut microbiota. www.frontiersin.org/journals/mic.... Great work of Mario Romero @migueldiezfdz.bsky.social @josete600.bsky.social and @rosacampo.bsky.social

A graphic showing E. coli and T7 bacteriophage floating in space. Image credit: C. Chitboonthavisuk.

Phage-host interactions have a role shaping microbial ecosystems, but what happens in #microgravity? Experiments aboard the #ISS reveal distinct evolutionary adaptations in T7 bacteriophage & #Ecoli, revealing #phage variants effective against resistant pathogens @plosbiology.org 🧪 plos.io/3LtKOfs

Bacteria chromosomes contain Genomic Islands that provide virulence, antibiotic resistance, MGE-defence,... They transfer between cells, but the mechanism of most remains elusive.

Here we explore the conjugative capacity of these mysterious Genomic Islands.

www.biorxiv.org/content/10.6...

A pleasure and an honour to have received this prestigious grant with my admired colleagues and friends @sanmillan.bsky.social and @asanchezlab.bsky.social . More about AMR under plasmid lens!. My sincere gratitude to @caixaresearch.bsky.social

@microryc.bsky.social @esgem-sg.bsky.social

🎉🎊🙌 😎

New preprint out! 📣🚨

We found that loss-of-function mutations in the carbapenem entry porin OprD of Pseudomonas aeruginosa do more than confer #AntibioticResistance: they reshape the bacterial membrane and interaction with the host, enhancing epithelial colonization capacity 🦠

#Microsky

Resistance mutation supply modulates the benefit of CRISPR immunity against virulent phages Only a fraction of bacterial genomes encode CRISPR-Cas systems but the selective causes of this variation are unexplained. How naturally virulent bacteriophages (phages) select for CRISPR immunity has...

New preprint!

Ever wondered why only a fraction of genomes encode CRISPR immunity? 🧬 🦠

Turns out CRISPR is rarely beneficial against virulent phages, being most beneficial against those for which resistance mutations are rare!

An epic effort by Rosanna Wright

www.biorxiv.org/content/10.1...

Phages with a broad host range are common across ecosystems - Nature Microbiology Proximity-ligation-based sequencing from 111 samples and 5 environments reveals that a substantial proportion of phages infect multiple species.

A fresh view on phage specificity, which challenges the traditional view of a narrow host spectrum of phages by unveiling that multihost associations are common across ecosystems kudos at @mmarbout.bsky.social &
@rkoszul.bsky.social www.nature.com/articles/s41...

🥳💖

I'm not sure how relevant it is to be included within the top 2% of scientists worldwide by Stanford-Elsevier Ranking, but I've climbed from 1.5% to 1% in the last year (Microbiology and Evolutionary Biology fields) and I´m very happy!

elsevier.digitalcommonsdata.com/datasets/btc...

Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens Nature Communications - This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the...

This work is finally published! 🥳🧬
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14

Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate Plasmids are autonomously replicating DNA molecules that stably coexist with chromosomes in bacterial cells. These genetic elements drive horizontal gene transfer and play a fundamental role in bacter...

New paper alert! 🚨

Plasmids promote bacterial evolution through a copy number-driven increase in mutation rate.

We combine theory, simulations, experimental evolution, and bioinformatics to demonstrate that mutation rates scale with plasmid copy number.

Let's dive in! 🧵👇

Do plasmids evolve faster 🐇, slower 🐢, or just like chromosomes 🧬?

In our new paper, we tackled this question using theory, simulations, bioinformatics, and experiments!

👇 Check out all the details in Paula’s thread!

Hint: 🐇 (most of the time)

This is a legitimately incredible result that hints at something fundamental about plasmids that we don’t yet understand

Proud of the talk of Iván Linares at the #FEMS2025 on hospital-associated microbiomes and its role on pathogens persistance 🦠 @femsmicro.org

Universal rules govern plasmid copy number - Nature Communications Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...

🚨🚨New paper out in @natcomms.nature.com!!

Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬

👉 www.nature.com/articles/s41...

Universal rules govern plasmid copy number - Nature Communications Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...

Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨

doi.org/10.1038/s414...

We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇

Happy International Day of Women and Girls in Science 👩‍🔬🧬 #womeninscience #girlsinscience

A plasmid-chromosome crosstalk in multidrug resistant enterobacteria - Nature Communications The authors describe a new crosstalk between a globally disseminated carbapenem resistance plasmid and clinical enterobacteria clones. This crosstalk provides a fitness advantage to the plasmid-carryi...

A plasmid-chromosome crosstalk in multidrug resistant enterobacteria

by Laura Toribio-Celestino, @sanmillan.bsky.social et al., in @naturecomms.bsky.social

www.nature.com/articles/s41...