We identified a new type of what seems to be a gene transfer agent in the most abundant gut microbe. Long read sequencing was the key here, as it showed suspiciously many 4kb and 8kb reads, which fits the smallest T=3 shrinked and T=3 regular capsids. And indeed, GTAs! www.nature.com/articles/s41...
Was invited to give a talk about tomography at ccp4 summer school. The most important slide is done
Same screen, three weeks later. At SLAC they sure know how to teach you to fib mill. Now, how to interpret the #tomo data?
My second visit to SLAC-Stanford to learn more about cryoET. Look at the size of that screen! This is going to be fun!
We still have space for additional attendees, and we encourage you to share this invitation within your networks so others can benefit from the fantastic panel we have assembled for this edition. Better program than your favourite paid conference - just saying 😉
Could an immune system control release of gene transfer agents? Contributed here with tomograms of Caulobacter (check the beautiful segmentations done by my PhD student Mai) showcasing how the cell envelope changes when the immune system is present vs absent.
What about your Easter Monday? #cryoem #remote #SLAC
First time I am seeing holes in my aromatics, collected at #SLAC temalpha #cryoem. Full story hopefully by the end of the summer!
Penton blooming of Ebor now on the cover of the April issue of mBio. #cryoET of #virus infection in toto
"You cannot take a dewar from York to Leeds without a car!" "Hold my pint, mate!" #cryoEM @astburycentre.bsky.social
Segmentation of a tomogram of the host cell infected by Ebor
In toto #CryoET shows that Ebor, the representative of a single jelly roll fold #virus lineage, uses five receptor-binding protrusions to bring a single #capsid penton closer to the #host membrane, triggering subunits dissociation from the centre - penton blooming.
journals.asm.org/doi/10.1128/...
#cryoEM #cryoET #virus #assembly PhD project at Uni of York, UK - apply by the end of tomorrow
www.findaphd.com?pj=164040
