28.02.2026 19:50
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“We present two platforms, single-cell outlining and transcriptome sequencing (scGOATseq) and GlycoScope…ScGOAT-seq quantifies lectin-accessible glycan states with gene expression, while GlycoScope enables multiplexed in situ co-detection of glycans and proteins in tissues.“ #Glycotime
“cathepsin K…uniquely degrades cell-surface mucins, proteoglycans, and polysialylated glycoproteins, and…reduces total glycocalyx thickness…a promising starting point for the development of a glycocalyx-debulking enzyme for cancer therapeutics.” #glycotime @carolynbertozzi.bskyverified.social 👏
We used to synchronize arabidopsis germination in the 4C/-20, maybe this will help here
thx for the boost! 🚀
Sheddase Targeting Chimeras (SHEDTACs) catalyze membrane target proteolysis https://www.biorxiv.org/content/10.64898/2026.02.06.703938v1
#TargetedProteinDegradation #InducedProximity #MolecularGlue #TPD #eTPD #PROTAC #LYTAC #MoDEA #KineTAC #PROTAB #AbTAC #EpiTAC #REULR #GlueTAC #TICTAC #AUTAC #ATTEC #AUTOTAC #TrogoTAC #RIPR #RIPTAC #AgnoTAC #FragTAC #SHEDTAC
This is precisely the direction we are pushing at my company, Ablytx Inc.
If you would like to learn more about what we are building at the company, or ways to become involved, please reach out and say hello!
bg@ablytx.com
Thank you for your time and feedback
At a high level, SHEDTACs add a third axis to the eTPD toolbox, distinct from proteasomal and lysosomal strategies, and create room to explore new protease-target pairings across disease areas.
· Functionally, enforced LAG3 shedding afforded enhanced TCR signaling beyond checkpoint blockade.
· This approach was extended to the non-canonical ADAM10 substrate, PD-1, suggesting induced shedding may be possible even for “unnatural” protease-target pairings.
...
· Using a focused SHEDTAC library, LAG3 shedding was robust across multiple epitope combinations on both LAG3 and ADAM10.
· Shedding was rapid and unaffected by perturbations of proteasomal or lysosomal pathways.
...
As a proof of concept, this study demonstrates induced shedding of the immune checkpoint LAG3 by the sheddase ADAM10 on primary T cells.
some key takeaways...
While eTPD has enabled new ways to access difficult targets, modern TPD approaches depend on endocytosis and intracellular trafficking. This work illustrates an alternative, more direct route - enforced proteolysis at the plasma membrane.
SHEDTACs are bispecific antibodies that bring endogenous membrane proteases (sheddases) into proximity with cell-surface targets to catalyze receptor proteolysis (shedding).
Dear friends and colleagues,
I am excited to share a preprint describing my take on extracellular targeted protein degradation (eTPD) - a proximity modality termed Sheddase-Targeting Chimeras (SHEDTACs).
www.biorxiv.org/content/10.6... 👀
At a high level, SHEDTACs add a third axis to the eTPD toolbox, distinct from proteasomal and lysosomal strategies, and create room to explore new protease-target pairings across disease areas.
· Functionally, enforced LAG3 shedding afforded enhanced TCR signaling beyond checkpoint blockade.
· This approach was extended to the non-canonical ADAM10 substrate, PD-1, suggesting induced shedding may be possible even for “unnatural” protease-target pairings.
...
· Using a focused SHEDTAC library, LAG3 shedding was robust across multiple epitope combinations on both LAG3 and ADAM10.
· Shedding was rapid and unaffected by perturbations of proteasomal or lysosomal pathways.
...
As a proof of concept, this study demonstrates induced shedding of the immune checkpoint LAG3 by the sheddase ADAM10 on primary T cells.
some key takeaways...
While eTPD has enabled new ways to access difficult targets, modern TPD approaches depend on endocytosis and intracellular trafficking. This work illustrates an alternative, direct route - enforced proteolysis at the plasma membrane.
SHEDTACs are bispecific antibodies that bring endogenous membrane proteases (sheddases) into proximity with cell-surface targets to catalyze receptor proteolysis (shedding).
A word cloud from the titles of ~1100 NSF grants that were terminated. The most frequent words are STEM, ADVANCE, Student, career, and engines.
This a word cloud from the titles of ~1100 terminated NSF grants.
It is hard not to see these grant terminations as an attack on the training of the future United States scientific workforce.
Amazing
Checkpoint blockade dependence on time of day (ToD)
“After…28.7 months, the median PFS was 11.3 months…in the early ToD group and 5.7 months…in the late ToD group”
Congrats, awesome figures and super comprehensive
On #WorldCancerDay 🎗️, our review on Writers and Readers of Sialylation in Immunoregulation in Cancer is out today in #JBiolChem:
🔗 www.jbc.org/article/S002...
Follow for future experimental glyco-work from the @siglecdude.bsky.social lab 🔬🧫 #glycotime
Desialylation potentiating immune escape #Glycotime
“We demonstrate… complement components (IgG, C1q, C4, and C5) and regulators (Factor I, Factor H, and C4bp) are sialylated, and that bacterial… desialylation suppresses complement activation…, thereby enabling complement evasion.”
The time of day for cancer immunotherapy is associated with major outcomes. Early is better. Results from a randomized trial of lung cancer, backs up the importance of our circadian rhythm and immune system
www.nature.com/articles/s41...
#resistandunsubscribe @profgalloway.com 👏
Apple & Google removed ICE tracking apps in their app stores.
TikTok reportedly suppressed videos criticizing ICE & the shooting of Alex Pretti.
Meta blocked Facebook groups tracking ICE & links to a database of agents that the government wants to keep secret.
Big Tech is enabling Trump's regime.
qualifying exam shenanigans