Gautier Courbon

Figure 1. Cryo-EM of abundant protein complexes in native membranes.

Figure 2. Cryo-EM of membrane proteins in vesicles.

Figure 3. 3D reconstruction of V-ATPase in native synaptic vesicle membranes.

Figure 4. Generation of membrane vesicles for structure determination of proteins in their native lipid bilayer.

I've written a review on what I think is an extremely exciting direction in cryo-EM:

Cryo-EM of endogenous membrane proteins in their native lipid bilayer

Open access in Quarterly Reviews of Biophysics:
doi.org/10.1017/S003...

Mapping the yeast atructural interactome with AlphaFold3: an open call for collaboration We are excited to announce the early-stage release of our S. cerevisiae  structural interactome mapping project. Using AlphaFold3 (AF3), w...

We have started a project trying to predic the interactions/structures of all yeast protein pairs using an AlphaFold pooling approach. We are making the current dataset open and we welcome collaborations.
www.evocellnet.com/2026/03/mapp...

Introducing our new blood-stage malaria vaccine immunogen based on the essential invasion protein PfCyRPA. By understanding where on PfCyRPA the best antibodies bind, we used structure guided design to generate PfCyRPA-EM, which contains only the epitopes of the best antibodies.

We are searching for a tenure-track junior faculty member. Join our collaborative research environment in the Department of Microbiology and Immunology at WCM. Please see the link to the job posting and distribute among your networks!
apply.interfolio.com/181869

Convergent MurJ flippase inhibition by phage lysis proteins - Nature A common mechanism of inhibition of the essential lipid II flippase MurJ by three distinct phage-encoded single-gene lysis proteins provides insights into potential new targets for antimicrobial development.

Nature research paper: Convergent MurJ flippase inhibition by phage lysis proteins

go.nature.com/4aAWHKb

In the first preprint from the lab, @sandaru-ileperuma.bsky.social demonstrates that the P. aeruginosa NQR complex supports virulence factors and identifies inhibitors of this complex. He also uncovered some interesting NQR dynamics controlled at the substrate binding site. tinyurl.com/yush599n

Structure of Acinetobacter baumannii cytochrome bo3 ubiquinol oxidase pubmed.ncbi.nlm.nih.gov/41759743/ #cryoEM

Discovery of inhibitors of the Pseudomonas aeruginosa NADH:ubiquinone oxidoreductase (NQR) that hinder virulence factors https://www.biorxiv.org/content/10.64898/2026.02.25.708042v1

Senior Scientist, Protein Sciences in South San Francisco, California, United States of America | Research & Development at Genentech Apply for Senior Scientist, Protein Sciences job with Genentech in South San Francisco, California, United States of America. Research & Development at Genentech

Our department at Genentech is hiring. We're looking for a freshly-minted PhD (0-3 years) protein biochemist / structural biologist to join as a Sr Scientist. A rare opportunity to join our group leader job family at a more junior level than we usually hire.

We’re hiring a Microscopy Core Director at the Wadsworth Center!
It's a state position (job stability & PENSION!) in a uniquely academic, research-driven environment 😎
Please share 🙏
statejobs.ny.gov/public/vacan...

Please spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳

mrc.tal.net/vx/mobile-0/...

Reminder to register for an upcoming in-person #CryoSPARC workshop in Toronto, delivered by @sickkidsto.bsky.social and the #CryoSPARC team, in partnership with the Canadian #CryoEM Nexus!
Deadline fast approaching!

The Structura logo with dates for the workshop listed. The bottom reads "Hosted by SickKids and Structura Biotechnology"

Deadline approaching to register for the @structurabio.bsky.social & @sickkidsto.bsky.social CryoSPARC workshop in Toronto!
Open to all, this is a unique opportunity to learn CryoSPARC from the experts at Structura.
April 30/May, 2026.
For more information and to register:
tinyurl.com/y42pf4wy

🚨 New preprint!

Together with @carobarisch.bsky.social lab, we investigated how intracellular compartmentalization shapes lipid acquisition in mycobacteria

Interestingly, deleting one FACL enzyme (FACL6) revealed striking compartment-dependent phenotypes 🔬

Check it out: doi.org/10.64898/202...

Thermophilic bacteria employ a contractile injection system in hot spring microbial mats Abstract. Bacterial contractile injection systems (CISs) are multiprotein complexes that facilitate the bacterial response to environmental factors or inte

Contractile Injection Systems - Hot Springs - cryoET: Read the final version of our paper here:
academic.oup.com/ismej/advanc...
#microsky #teamtomo

Long in the making, but happy to present the Chlamydomonas chlororibosome!
Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins.
bioRxiv 📖: shorturl.at/q44tG
This suggests greater chlororibosome diversity than expected!
1/n 🧵

🧪🧬New preprint We present cryo-EM structures of reconstituted CTCF–nucleosome complexes, showing CTCF dimerization drives nucleosome oligomerization into defined higher-order assemblies. Disrupting CTCF–CTCF interfaces in mESCs reduces looping and impairs differentiation. tinyurl.com/CTCF-nucleos...

Cartoon representing the mycobacterial oxidative phosphorylation system, including cytochromes, NDH-2, and ATP synthase. Taken from Figure 1 of the article.

#Microbiology

The anti-tuberculosis drug bedaquiline targets the mycobacterial ATP synthase, but how that leads to bacterial death is unclear

Here the authors clarify how bedaquiline works

@jnblab.bsky.social @morwan.me
#TBSky #antibiotics #AMR

http://dlvr.it/TQjZ0W

Crystal structure of malaria parasite fusogen PfHAP2 domain 3 and nanobodies - unsolved mysteries galore! 🦟🦙💎

PfHAP2 is part of family of ancient fusogens involved in fertilisation processes in algae, plants to malaria parasites.

Check out the first crystal structure of PfHAP2 D3 and nanobodies

Dear Colleagues, As part of the Canadian Cryo-EM Nexus, The Hospital for Sick Children (SickKids) and Structura Biotechnology are hosting a two-day CryoSPARC workshop at SickKids in Toronto on April 30 and May 1, 2026. Led by experts from Structura, this workshop offers a unique opportunity for participants with prior CryoSPARC experience to deepen their skills and become a key resource for cryo-EM image analysis within their institutions. Applicants should have hands-on experience with CryoSPARC, demonstrated by having processed at least two datasets, and must be committed to sharing their expertise with colleagues upon returning to their home institution. There is no charge for participating in the workshop, which is sponsored by SickKids and Structura. Attendees will need to bring their own laptop computer and arrange for travel, accommodation, and meals. Given the limited capacity (15 students), we kindly request that interested individuals ensure that they can cover these costs before registering. Please register before February 20, 2026 using the following link: https://sickkids.cventevents.com/event/a0aebb07-66e6-4cbe-b471-8009fe0c227c/summary?i=97c975e9-4bac-42b9-a1b2-95974110db9e&tm=DXVv8zGvH5uA7nqj8lEF4iifi-ukLal5QBBjdXWGD2E&locale=en-US We look forward to welcoming you to this workshop. Sincerely, Samir Benlekbir and John Rubinstein (SickKids) & CryoSPARC Team (Structura)

@structurabio.bsky.social and @sickkidsto.bsky.social CryoSPARC workshop in Toronto!
April 30/May 1st 2026.
Part of the Canadian Cryo-EM Nexus.
Please register here: sickkids.cventevents.com/event/a0aebb...

Cryo-EM structure of bixafen-bound S. cerevisiae complex II unravels SDHI specificity against pathogenic fungi pubmed.ncbi.nlm.nih.gov/41606097/ #cryoEM

Our paper on the structural and functional basis of the human dimeric OS9–SEL1L–HRD1 ERAD complex, together with @qilabuva.bsky.social, is now out! Huge congratulations to Leo, Emir, and Elina!
www.nature.com/articles/s41...

Four representative slices of cryo-tomograms showing annotated organelles.

New on the CryoET Data Portal: ~27,000 tomograms of affinity-captured lysosomes from HEK293T cells across 4 physiological states. Includes raw data, AreTomo3 reconstructions & Membrain-Seg annotations. Openly available for reuse!

cryoetdataportal.czscience.com/depositions/...

🚀 CryoSPARC v5.0 BETA is here!

We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!

Full changelog: cryosparc.com/updates/v5.0.0

This is figure 1, which shows that ASCT enables high-throughput assessments of bacterial viability.

A paper in Nature Microbiology presents Antimicrobial Single-Cell Testing, a large-scale live-cell imaging approach that quantifies bacterial killing in real time at single-cell resolution, which can predict treatment outcomes. go.nature.com/3Nwnez8 #microsky 🧪

Excited to share our latest work on dissecting the mechanism of processive telomeric DNA synthesis by telomerase. Led by amazing PhD student Sebastian Balch in collaboration with lab members,@automnenine.bsky.social @rachael-kretsch.bsky.social,@rdaslab.bsky.social.
www.nature.com/articles/s41...

Cryo-EM structure reveals how influenza A virus NEP binds the viral polymerase at a regulatory hotspot, coordinating RNA synthesis and nuclear export. Fantastic collaboration with @loiccarrique.bsky.social and Jon Grimes. www.science.org/doi/10.1126/...

We’re excited to share our latest preprint on the mechanism of excised linear intron stabilization in yeast! This work was led by PhD student @glennli.bsky.social and was a wonderful collaboration with @maxewilkinson.bsky.social. Link: www.biorxiv.org/content/10.6... (1/4)

Lonsdale's notebooks, showing incredibly neat and consistent handwriting

Lonsdale's notebooks, showing incredibly neat and consistent handwriting

We couldn't let World Handwriting Day go by without sharing Kathleen Lonsdale's penmanship ✍️

Lonsdale conducted pioneering research in X-ray crystallography and worked at the Ri. In 1929, she proved that the structure of benzene was flat—the chemical was also discovered here, by Michael Faraday.

Postdoctoral Researcher - Structural Studies - Dr Suyang Zhang - LMB 2761 - Cambridgeshire (GB) job with MRC Laboratory of Molecular Biology (LMB) | 12852642 Postdoctoral Researcher Salary £42,694 per annum  MRC Laboratory of Molecular Biology, Cambridge, UK We are recruiting a Postdoctoral Researcher to...

🚨Postdoc opportunity🚨Our lab at MRC-LMB Cambridge is recruting a postdoc to study transcription-coupled splicing using cryoEM and endogenous isolation! 🔬 🧬

Please feel free to get in touch directly #postdoc #cryoEM #biochemistry #splicing

Apply here by 11 Feb
www.nature.com/naturecareer...