Edismauro Freitas-Filho, Ph.D.

Autophagy-lysosome pathways are known targets of intracellular bacteria – Ivan Đikić @idikic.bsky.social et al find Legionella uses phosphoribosyl-ubiquitination via SidE to alter autophagic SNAREs & create a bacteria-containing vacuole unable to fuse with lysosomes
www.embopress.org/doi/full/10....

Phosphoribosyl ubiquitination of SNARE proteins regulate autophagy in Legionella infection https://www.biorxiv.org/content/10.1101/2025.05.19.654886v1

Mitochondria–membranous organelle contacts at a glance Summary: We provide an overview of contacts between mitochondria and other membranous organelles, describing the interorganelle tethers involved and the factors that regulate the composition and funct...

journals.biologists.com/jcs/article/...

a man in a suit and tie is sitting at a table with his eyes closed and a pile of food .. ALT: a man in a suit and tie is sitting at a table with his eyes closed and a pile of food ..

#Autophagy and #mTOR papers and preprints from last week. As always a great selection, enjoy! 👌

biomed.news/bims-auttor/...

Retromer promotes the lysosomal turnover of mtDNA Lysosomal uptake of mtDNA controls mitochondrial quality.

Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵

www.science.org/doi/10.1126/...

a man in a suit and tie is asking if he read it ALT: a man in a suit and tie is asking if he read it

#Autophagy and #mTOR papers from last week, lots of relevant stuff as always, enjoy!

biomed.news/bims-auttor/...

RAB5c controls the assembly of non-canonical autophagy machinery to promote phagosome maturation and microbicidal function of macrophages https://www.biorxiv.org/content/10.1101/2025.03.25.645097v1

Florey Lab = Science + Good Vibes™️ 🧪✨ Beyond grateful for the collaboration… and thrilled to say I’ll be back this year! (Stay tuned for more 🔥)

Mycobacterium tuberculosis phagosome Ca2+ leakage triggers multimembrane ATG8/LC3 lipidation to restrict damage in human macrophages Ca2+ leakage triggers LC3 lipidation on multimembrane as Mtb phagosome damage response, independent of autophagy.

Happy to share the latest work from the lab @crick.ac.uk. Outstanding work from Di Chen and Tony Fearns showing the link between Mtb membrane damage, calcium leakage and ATG8/LC3 lipidation in macrophages. Congratulations Di and Tony!
www.science.org/doi/10.1126/...

Exciting news from the lab out today in Science - the structure of human PINK1 on a TOM-VDAC complex!
@wehi-research.bsky.social

"Big thanks to @floreylab.bsky.social for the awesome collaboration—couldn’t have done it without you! 🙌 #TeamScience"

🔑 Key findings:
RAB5c is essential for non-canonical autophagy machinery assembly.
It fine-tunes ROS production & V-ATPase recruitment.
Disrupting the RAB5c-V-ATPase-ATG16L1 axis impairs pathogen clearance in vivo.

🚀Our latest work reveals how RAB5c orchestrates LC3-associated phagocytosis (LAP) to boost macrophage defense against A. fumigatus! 🦠🔬
🧵 Dive into the mechanisms linking endosomal traffic to innate immunity!
#Immunology #Autophagy #Phagocytosis #Macrophage #Preprint
doi.org/10.1101/2025...