Ishier Raote

A young barmaid behind the bar at the famous French nightclub Folies-Bergère is staring forward in a bored, introspective way. Behind her is a mirror which reflects the lively scene. At the top left you see the green shoes of a trapeze artists. More importantly, at right you see the back of the barmaid, and see that she is face to a man who leans close and seems to be requesting something. This is Manet's famous painting "A Bar at the Folies-Bergère": https://en.wikipedia.org/wiki/A_Bar_at_the_Folies-Berg%C3%A8re

The perspective looks all wrong. You're staring straight at this barmaid, but her reflection in the mirror is way off to right. Even worse, her reflection is facing a guy who doesn't appear in the main view!

But in 2000, a researcher showed this perspective is actually possible!

(1/n)

India won their second cricket T20 World Cup in a row and I caught myself thinking: “Focused strategy, complementary expertise, efficient teamwork, ambitious objectives, coordinated execution, consistent performance, high-impact outcome…”
Grant application in progress, obviously.

Yup, heat could also make it go bad. It might also look yellowish, in addition to being more viscous and autofluorescent.
For once I'd follow the instructions, "store in a cool, dark, dry place!"

I'd just get a new bottle; this one's gone bad. Immersion oil degrades with heat, light, air, or maybe even some contamination got in (water, old oil from a slide).

strong illumination (esp. UV / blue) can photo-oxidize some immersion oils. That can increase autofluorescence and make the oil thicker / viscous over time. Often happens with older/contaminated oil.

Best buddies? More like budding buddies 🪼👯‍♀️✨ Six stages of asexual budding in Hydra to generate new genetically identical individuals (clones). 🟣: collagen 🟢: stem cells. 📸 Image by Ben Cox #FluorescentFriday

I know this guy very well because it's me! 😅

Thank you for this great opportunity!

We are very proud to present our newest paper on the role of Wnt signalling in abscission dynamics in stem cells! A brief thread to entice you to read Snježana’s exciting foray into signalling.

I had fun writing this N&Vs on two fascinating new papers on centromere evolution in yeast. If you’re interested in how centromeres adapt yet stay functional, here’s my take. rdcu.be/e6DD0 @nature.com @gautamdey.bsky.social @gsherloc.bsky.social @helsenjana.bsky.social @maxhaase.bsky.social

My writing technique

The Golgi Network, Volume II This book explores the interaction of Golgi with different organelles such as ER, the vesicular system, and protein complexes that execute these tasks.

A wonderful collection of contemporary topics and perspectives on secretion and Golgi biology edited by Akihiko Nakano and Jaakko Saraste.
The Golgi Network, Volume II @SN and link.springer.com/book/10.1007...

But The Matrix was released when I was finishing high school.

Very recent movie.

CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly - Nature CRISPR–Cas9 screening identifies CLCC1 as a factor that increases neutral lipid flux to prevent hepatic steatosis and promotes nuclear pore complex assembly by promoting membrane bending and fusi...

Sooo happy to share our new paper in @nature.com “CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.” A terrific collaboration with @arrudalab.bsky.social, led by co–first authors Alyssa Mathiowetz and Emily Maymand.
www.nature.com/articles/s41...

This is happening tomorrow! Looking forward to seeing all the #membranes enthusiasts on zoom!

Just a few days left for this application deadline!

Having just seen Gautam speaking in Edinburgh yesterday, can highly recommend!

After he has spoken at the Institut Jacques Monod, definitely.
I see you too are coming soon!

Institut Jacques Monod Seminar
Friday, Feb 27
Institut Jacques Monod

Gautam Dey @gautamdey.bsky.social (EMBL, Heidelberg) Evolution and diversity of mitosis

Exciting evolutionary cell biology: bridging genomics, experimental evolution, and stunning imaging across diverse protozoans
buff.ly/uCSfjgQ

#membrane seminars are back this week wirh Manu @deriverylab.bsky.social and Andela Šarić! Join us on Thursday at 5 pm CET spread the word!

#VAMP7-dependent late endosomal #secretion of #ER and #mitochondrial proteins impacts the #tumor #microenvironment and #macrophage engagement
www.nature.com/articles/s41...

A multidisciplinary framework for protein trafficking: tackling unanswered questions

A study on the effect of being a PI on greying hair. Correlation or causation? (Also an article on the Roadmap published last year in NRMCB)

www.unamur.be/en/newsroom/...

Join us next Tuesday for the next @dgz.bsky.social workshop on #trafficking and EVs! Spread the word please!

𝙈𝙚𝙢𝙗𝙧𝙖𝙣𝙚𝙨 𝙖𝙣𝙙 𝙩𝙝𝙚𝙞𝙧 𝙡𝙞𝙥𝙞𝙙𝙨 𝙖𝙣𝙙 𝙥𝙧𝙤𝙩𝙚𝙞𝙣𝙨 𝙞𝙣 𝙤𝙧𝙜𝙖𝙣𝙚𝙡𝙡𝙚 𝙗𝙞𝙤𝙜𝙚𝙣𝙚𝙨𝙞𝙨 - Spetses, Greece - May 2026 | Utrecht University Utrecht University

CALLING PHDs AND POSTDOCS! Apply for the 20th FEBS/EMBO summer school on Membranes lipids & proteins in organelle biogenesis! Spend a glorious week with leaders in the field on the beautiful island of Spetses in Greece this May. Registration: spetses2026.sites.uu.nl @embo.org @febspress.bsky.social

📢 Institut Jacques Monod Seminar

📅 February 27th
📍 Institut Jacques Monod

Invited by the @ishier.bsky.social, @gautamdey.bsky.social will present the Institut Jacques Monod seminar "Evolution and diversity of mitosis"

➡️ buff.ly/uCSfjgQ

A model to describe what we show in the paper. INVs are important for sequestering GLUT4 and related proteins into GSVs, the vesicles that are mobilised in response to insulin.

🚨 We have a new preprint out! 🧪

In this paper we look at GLUT4 trafficking. This is a short explainer thread, but please read the paper!

1/n 🧵

www.biorxiv.org/content/10.6...

Cells come in many shapes and sizes, with diverse physiological functions. But how do #organelles and their interaction networks remodel during #differentiation of stem cells into different cell types? Here’s what we discovered about neuronal differentiation: 1/13

Where does the rest of the GSV content come from - is it an ERGIC-derived membrane fusing with the INV?
GSVs have been suggested to be able to bypass the Golgi en route to secretion, is that a different pool of GSVs?
Is there any TUG on these GSVs? Looking forward to how this develops!

Nice preprint! We still don’t know how insulin-responsive GLUT4 storage vesicles (GSVs) are made. @steveroyle.bsky.social shows a subset of intracellular nanovesicles sort and retain GLUT4, supplying components that build these unique vesicles.
www.biorxiv.org/content/10.6...

Bet it isn't as brilliant as Calvin

Cellularization flips the switch between multinucleate and multicellular states, and at least in opisthokonts, the group that includes animals and fungi, the toolkit + recipe are conserved.

Another fun Dudin-Dey team-up with many in both labs and EMBL core facilities pitching in