AmpliPhy improves gene trees by adding homologs without affecting alignments
In phylogenomics, gene tree reconstruction depends on multiple sequence alignment (MSA) and tree inference, and ongoing work continues to improve inference quality. Denser taxon sampling has been associated with improved gene tree inference, suggesting that adding homologs could be a practical route to higher accuracy as sequence databases continue to expand. However, adding sequences can influence multiple steps of typical inference pipelines, and little is known on its specific effect on the multiple sequence alignment, tree reconstruction, and rooting steps. We performed a large-scale empirical benchmark to quantify how homolog enrichment affects alignment and phylogenetic inference. Using an enrichment-impoverishment design and a measure of tree accuracy based on taxonomic congruence, we found that enrichment consistently improves tree inference quality, while effects on alignment quality are marginal. We show that this improvement is associated with accurate root placement on enriched trees when sensitive homolog search is accompanied. Notably, much of the benefit can be retained with relatively compact alignments produced by sequence addition. Building on these observations, we provide a tool, AmpliPhy, which efficiently improves phylogenetic reconstruction of protein families through homolog enrichment. The AmpliPhy open-source pipeline software is available at https://github.com/DessimozLab/ampliphy. ### Competing Interest Statement The authors have declared no competing interest. Swiss National Science Foundation, https://ror.org/00yjd3n13, 216623, 10005715
Can ever-increasing sequence databases improve phylogenetic reconstruction of a gene family? Our new preprint introduces AmpliPhy, a pipeline that automates homolog enrichment to improve gene tree inference, built on a robust phylogenomic benchmark scheme. ๐งต1/n
๐ doi.org/10.64898/2026.01.26.701724
28.01.2026 06:10
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Gene annotations matter for downstream analyses: different structural gene-annotation pipelines yield markedly different orthology calls. We compare four strategies across species and argue for standards & QC before inference. Work led by @silviaprietob.bsky.social. Paper: doi.org/10.1093/bioi...
23.10.2025 06:20
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Happy to see my illustration featured on the cover!
07.11.2025 09:42
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Our lab has published a couple of major papers in the past few months, which I would like to highlight in this thread! ๐งต
23.10.2025 06:10
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Here are all the talks I attended and sketched at #ESEB2025 ! Only a fraction of the 642 speakers at the conference ๐ฎ
I had an absolutely amazing week chatting about lots of fascinating science
#sciart #eseb #esebcongress @eseb2025.bsky.social
02.09.2025 09:11
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You can find my poster "Microsynteny and functional relatedness in eukaryotes" at the slot 209 today and visit at around 5 if you want to know more or talk anything synteny, gene order, functional association or genome evolution related :) #ESEB2025
19.08.2025 12:32
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@irenejulca.bsky.social presenting the results unveiling the functional fate of duplicates genes at #ESEB2025 !
19.08.2025 12:07
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It's been an exciting first day of #ESEB2025, with a great opening talk by Michael Lynch. Moreover, a very timely recap on lessons learnt in pines climate change adaptations by Santiago Gonzรกlez, during a week marked by unprecedented wildfires in Spain. [Among many, many other interesting talks]
18.08.2025 22:19
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Another consequence of the disastrous attack on NIH by Trump, Musk, and their followers
03.07.2025 14:09
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This work is partly limited by a poorly resolved phylogeny and so future work will look to revisit these results with an improved phylogenetic framework. Nonetheless, we hope our extensive literature synthesis and results will facilitate further work in this under-studied molluscan clade ๐๐.
03.07.2025 08:00
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Surprisingly, we found no evidence that diet choice, chemical acquisition and colour pattern evolved together.
03.07.2025 08:00
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What was the dorid ancestor like? Likely a sponge-eater ๐งฝ sequestering its metabolites, with a flair for fashion: showing complex colour patterns with spots or stripes โจ๐๐. This trend remains common in modern dorids (figs 1 & 3).
03.07.2025 08:00
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We compiled data on prey preference ๐, chemical acquisition method ๐งช and colour pattern ๐จ across 88 dorid genera.
Using published sequence data, we reconstructed a phylogeny of the Doridoidei to investigate the evolution of these traits in tandem.
03.07.2025 08:00
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Image corresponding to Figure 1 in the manuscript. The colour patterns diversity in dorid nudibranchs is displayed by 25 images of different dorid species.
Nudibranchs are known for housing diverse chemical compounds and for their eye-catching colour patterns. These features are key in predator deterrence and signaling.
03.07.2025 08:00
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Tracing the evolution of key traits in dorid nudibranchs
Reconstructing trait evolution is critically important for elucidating the processes generating biodiversity. However, this work is in its infancy in non-model clades for which we lack a basic underst...
Check out our recent paper โTracing the evolution of key traits in dorid nudibranchsโ! ๐งฌ๐ dx.plos.org/10.1371/jour...
Nudibranchs are a fascinating and diverse group of molluscs, unique and interesting systems in ecology and evolutionary biology.
@molluscular.bsky.social
03.07.2025 08:00
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Unicore is now published on GBE ๐
Unicore rapidly identifies structural single-copy core genes from input species proteomes for phylogenetic analysis. Powered by Foldseek and ProstT5, Unicore enables linear-scale structure-based phylogeny of any given set of taxa. ๐งต1/n
๐ doi.org/10.1093/gbe/evaf109
03.06.2025 06:54
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Computational Biology Symposium
After 6 (healthy) months of hiatus from social media, first post with a brand new conference announcement!! ๐ฅCOMPUTATIONAL BIOLOGY IS EVERYWHERE! Join us in Lausanne for the first international Computational Biology Symposium! 18-19 September 2025 Registrations are OPEN! cbiosymposium.unil.ch
18.03.2025 13:08
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