Gokul Upadhyayula

Assistant/Associate/Full Project Scientist - Instrumentation Microscopy – Advanced Bioimaging Center – Department of Molecular and Cell Biology University of California, Berkeley is hiring. Apply now!

We are recruiting an instrumentation scientist.
aprecruit.berkeley.edu/JPF05253

Imaging spotlight: Volumetric Imaging via Photochemical Sectioning (VIPS) - FocalPlane Imaging spotlight: Volumetric Imaging via Photochemical Sectioning (VIPS) - Tools

🔬🔦Check out our ‘Imaging spotlight’ on Volumetric Imaging via Photochemical Sectioning (VIPS) from @ruixuan3.bsky.social, Srigokul Upadhyayula and colleagues.

VIPS helps researchers scale super resolution imaging to large whole-mount tissues.
focalplane.biologists.com/2025/12/02/i...

Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structure...

(1/x) Excited to report VIPS (Volumetric imaging of biological specimens via photochemical sectioning) @science.org! VIPS uses light-based sample sectioning to eliminate the inherent imaging depth limit of high-resolution optical microscopes @supgokul.bsky.social: www.science.org/doi/10.1126/...

This work was made possible in part by support from
@czbiohub.bsky.social @cziscience.bsky.social
@sloanfoundation.bsky.social, @berkeleylab.lbl.gov, @hhmi.org, @nersc.bsky.social, Philomathia Foundation and NIH.

Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structure...

www.science.org/doi/10.1126/...

Cover: 3D WT bulb, wedge-out view; VIPS alternates light-sheet imaging & photochemical sectioning. #ExM #LLSM #imaging #neuroscience

Thrilled to share our Science cover! VIPS (volumetric imaging via photochemical sectioning) eliminates working-distance limits for whole-mount, nanoscale imaging. LLSM + photodegradable hydrogel + petabyte-scale compute → mapped axons & myelin across two mouse olfactory bulbs (WT vs NPC1).

Fourier-based three-dimensional multistage transformer for aberration correction in multicellular specimens - Nature Methods Adaptive optical vision Fourier transformer (AOViFT) is a machine learning-based framework for accurately inferring aberrations and restoring diffraction-limited performance in diverse biological specimens.

Adaptive optical vision Fourier transformer (AOViFT): a machine learning framework for inferring image aberrations and restoring diffraction-limited performance.

www.nature.com/articles/s41...

Finally here! Our preprint on the MOSAIC, a multimodal adaptive optical microscope enabling non‑invasive in‑vivo imaging from molecules to organisms, is out. MOSAIC captures everything from subcellular dynamics to neural activity in live mice.
www.biorxiv.org/content/10.1...

A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms https://www.biorxiv.org/content/10.1101/2025.06.02.657494v1