📢 Happy to share our new preprint from @sophiemartinlab.bsky.social !
Check our latest work on cell–cell fusion, uncovering how PAK kinases promote cell-cell fusion irreversibility by preventing cell wall repair.
Huge shoutout to
@sudiya14.bsky.social, Deepak, @nishaantpb.bsky.social, Sanjana, Sucheta, and Angana for their incredible efforts - couldn’t have done it without you!
Incredibly grateful to @syncellbiolab.bsky.social for the opportunity to do this amazing work!
IntAct-U-ExM: Ultrastructure Expansion microscopy of actin networks via an internally-tagged actin https://www.biorxiv.org/content/10.1101/2025.05.14.654030v1
Thank you very much. We are super excited to contribute to the field of actin cytoskeleton with our tool!
Lovely work from Saravanan and colleagues, they insert the 13aa alfa tag into actin and then use an Alexa-nanobody to monitor actin in expansion microscopy in yeast and human cells. This is an excellent tool for actin research, and the general principle can be applied to a variety of other proteins.
Beautiful images 🤩
You did it @syncellbiolab.bsky.social !! Amazing .
Congrats to the Team and what gorgeous imaging ...
Wow
Nice cool study, @syncellbiolab, @anubhav_dhar, @SuchetaDey69384, @MullickSan22454, @Nishaant_PB, @deepak_iisc and Angana. Wonderful to be collaborating with you on this work and looking at #actin #isoforms in #yeast and #mammalian_cells using #UExM.
Super excited to share our latest work on visualising actin networks through ultrastructure expansion microscopy. A major leap towards high-resolution visualisation of the actin cytoskeleton. Grateful to @syncellbiolab.bsky.social and @anubhavdhar.bsky.social for giving me this opportunity!
This method reveals actin patches, cables, rings (yeast), and cortex, filopodia, lamellipodia, stress fibers (cell lines). Notably, it captures nuclear actin filaments with high specificity highlighting IntAct-U-ExM’s power to resolve nanoscale actin architecture across species.
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
