Light-Sheet Fluorescence Microscopy (LSFM) course's Avatar

Light-Sheet Fluorescence Microscopy (LSFM) course

@lsfm-na

This is the US version of the long running course Pavel and Emmanuel have been running. We are just this side of the pond. Being held at MDIBL in Bar Harbor, Maine, June 2-6, 2025.

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Latest posts by Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na

This was a great sample to check out -- who knew 100M year old amber would be so close to Ethyl Cinnamate in RI? Huge shout out to @brukercorporation.bsky.social for the fleet of lightsheets at @lsfm-na.bsky.social last week!

09.06.2025 19:30 πŸ‘ 7 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0
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For #MicroscopyMonday, a 100M year-old 🦟 in amber! Lots of familiar #NESM faces up in Acadia for the #LSFM25 at the MDI @mdibiolab.bsky.social !

πŸ”¬: Bruker @brukercorporation.bsky.social, LCS SPIM
🟠: Courtesy the Sims Collection
πŸ₯Ό&πŸŽ₯: Jan Frankowski @frankowskijc.bsky.social

@lsfm-na.bsky.social

09.06.2025 16:03 πŸ‘ 32 πŸ” 8 πŸ’¬ 0 πŸ“Œ 1
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And with that, #lsfm25 comes to a close! We want to thank everyone who helped make this possible - the faculty, course directors, TAs, vendors, and sponsors. But most importantly the many wonderful people at MDIBL. See you all next year!

07.06.2025 13:39 πŸ‘ 3 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Preparation for the final event of the course: a boat tour of Acadia!

06.06.2025 21:57 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Now that the students have had several days to learn sample preparation and image acquisition, we are wrapping up day five with a crash course called "Now what do you do with your data?" Led by @dpshepherd.bsky.social and Carsten Wolff.

#lsfm25 #microscopy #science #education

06.06.2025 17:59 πŸ‘ 4 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
Adaptive optics oblique plane microscopy of 100 um thick mouse brain slice with immunofluorescence.

Adaptive optics oblique plane microscopy of 100 um thick mouse brain slice with immunofluorescence.

Spending some time at #lsfm25 cleaning up code. It now has distributed GPU gradient-consensus RL decon (@jamesdmanton.bsky.social @andrewgyork.bsky.social) our fast orthogonal deskew, and stitching/fusion to ome-ngff v0.5. Example AO-OPM data on uncleared mouse brain slice github.com/QI2lab/opm-p...

04.06.2025 18:56 πŸ‘ 23 πŸ” 3 πŸ’¬ 0 πŸ“Œ 0
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Up in Acadia at the @mdibiolab.bsky.social for #LSFM25! Here's a cleared whole mouse head on the SmartSPIM, 3.6X objective for #3DThursday.

πŸ”Ž @jtakatoh.bsky.social, now at @stonybrooku.bsky.social
πŸ”¬ LifeCanvas Technologies, lifecanvastech.com/products/sma...
πŸ₯½ syGlass.io

@lsfm-na.bsky.social

05.06.2025 12:54 πŸ‘ 12 πŸ” 6 πŸ’¬ 0 πŸ“Œ 0
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We are so excited to start day four with a wonderful talk from Kara Cerveny!

#lsfm25 #science #microscopy #education
@mdibiolab.bsky.social
@kara-cerveny.bsky.social

05.06.2025 13:01 πŸ‘ 4 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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It's day three and we are starting our morning off with an excellent intro to clearing by Doug Richardson.

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social

04.06.2025 13:48 πŸ‘ 3 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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And it begins....

03.06.2025 23:00 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

The calm before the storm indeed! In just a half an hour, this board will be bombarded by 25 students rushing to grab a spot on their instrument of choice. 11 systems. Only 9 labs. May the odds be ever in their favor...

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social

03.06.2025 22:30 πŸ‘ 2 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0
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We are back from lunch with a talk from Raghav Chhetri telling us all about using light sheet microscopy for neuroscience imaging!

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social
@rchhmiscope.bsky.social

03.06.2025 17:41 πŸ‘ 4 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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It's day two! We're starting off a day filled with science with a talk from Anna-Karin Gustavsson from Rice University.

#lsfm25 #microscopy #science #education

@mdibiolab.bsky.social
@riceuniversity.bsky.social
@gustavssonlab.bsky.social

03.06.2025 13:11 πŸ‘ 5 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0
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Morning in Bar Harbor doesn’t suck.

03.06.2025 10:25 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Step 8: In @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Well day one done worth a sunset!

03.06.2025 01:59 πŸ‘ 4 πŸ” 1 πŸ’¬ 1 πŸ“Œ 0
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Step @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Went to collect some samples... Here is a pink tunic ate!

02.06.2025 19:31 πŸ‘ 9 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0
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Reto Fiolka and OPM for the Keynote!

02.06.2025 21:08 πŸ‘ 5 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
Fiolka Lab | UT Southwestern, Dallas, Texas The long-term goal of Fiolka Lab's researchΒ is to develop and implement imaging technologies that provide unprecedented insight into cancer biology.

labs.utsouthwestern.edu/fiolka-lab

02.06.2025 20:52 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

With the end of our first round of vendor talks behind us, we are excited to welcome Reto Fiolka as our keynote speaker this year.

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social

02.06.2025 20:52 πŸ‘ 2 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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Holly Gibbs discussing the logistics of image processing and analysis

02.06.2025 17:37 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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While we are on our lunch break, the students are enjoying some beautiful weather here at @mdibiolab.bsky.social.

#lsfm25 #microscopy #science #education

02.06.2025 17:04 πŸ‘ 4 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0
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We now have Vera Morales from Nikon telling us all about objectives: the why, when, and how!

#lsfm25 #microscopy #science #education

02.06.2025 15:02 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Claudette St Croix from CBI kicks off LSFM 2025 at @mdibiolab.bsky.social

02.06.2025 13:22 πŸ‘ 6 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0

We are thrilled to have you, Melissa!

02.06.2025 13:08 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

@lsfm-na.bsky.social Is this heaven, or am I just currently surrounded by ELEVEN DIFFERENT LIGHT SHEET MICROSCOPES? So thrilled to be out here in Acadia chatting about microscopy!

02.06.2025 13:01 πŸ‘ 4 πŸ” 3 πŸ’¬ 2 πŸ“Œ 0
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LSFM 2025 has kicked off with an introduction from the one and only, @glaevsky.bsky.social, our course director.

#lsfm25 #microscopy #science #education

02.06.2025 12:53 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Calm before the storm…

01.06.2025 20:50 πŸ‘ 18 πŸ” 3 πŸ’¬ 0 πŸ“Œ 1

You will be missed Ed. And thank you!

01.06.2025 13:12 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Time to sheet!

01.06.2025 13:10 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Why no swim?!

31.05.2025 21:04 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0