Our work now on its final version. We mapped the mechanical properties of roots at tissue and single cell levels using Brillouin microscopy and molecular rotors. Additional mutants and stress measurements from what we previously showed in the preprint are included.
www.science.org/doi/10.1126/...
7/7 If you have any feedback or questions about the article, do not hesitate to get in touch!
6/7 Strikingly, only two directly interacting proteins have been described for THE1 (GEF4 and the ligand RALF34). We propose that identifying other interacting proteins is key for a better mechanistic understanding of THE1.
5/7 Don't forget about the supplemental figures, where we show which post-translation modifications are conserved and different between THE1 orthologues and other CrRLK1Ls.
4/7 We also provide a new overview of the available mutants and possible post-translational modifications, of which the function remains to be tested.
3/7 We find that THE1 already diverged from the other CrRLK1Ls in the earliest stage of angiosperm evolution and is conserved in angiosperms, but is notably absent in grasses.
2/7 THE1 was discovered almost 20 years ago as a mediator of the response to cellulose biosynthesis inhibition. Since, several new roles have been described in hormone signaling, linking cell wall integrity to immunity, and determining mechanical properties of cell walls.
1/7 Happy to share that the first publication from my PhD, in which we review the literature about THESEUS1, is now out in @theplantjournal.bsky.social ☘️: doi.org/10.1111/tpj....
Fig. 1.Schematic overview of pattern-triggered immunity (PTI) in plants upon pest infestation. Cell wall-degrading enzymes (CWDEs) secreted by herbivory disrupt cell wall integrity, releasing damage-associated molecular patterns (DAMPs). The defence response is activated by the recognition of these DAMPs, as well as herbivore-associated molecular patterns (HAMPs), by pattern recognition receptor complexes (PRRs and co-PRRs). Elicitor binding to PRRs leads to a series of signalling events, such as membrane depolarization (Vm), activation of Ca2+ channels and influx of Ca2+ into the cytosol, burst of reactive oxygen species (ROS) via activation of RBOHD, membrane depolarization, and activation of the mitogen-activated protein kinase (MAPK) cascade. MAPK and Ca2+-dependent kinase (CDPK) cascades trigger transcriptional reprogramming, activating phytohormone (SA, JA, and ET) signalling, transcription regulatory factors, and synthesis of defensive compounds.
🪲 REVIEW 🪲
This review uncovers the critical role of the plant cell wall in pest defence, integrating structural, molecular, and genetic insights to highlight its emerging significance in plant–pest interactions during herbivory - Perez-Alonso et al.
🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪
Fig. 1.The functional diversity of plant cysteine-rich peptide families, RALF, and EPF/EPFL in Arabidopsis. The representation of various growth and developmental roles of well-characterised functionally RALF and EPF members based on their expression in different Arabidopsis organs. EPF, Epidermal Patterning Factor; EPFL, EPF-Like; RALF, Rapid Alkalinization Factor.
🌱 SPECIAL ISSUE REVIEW 🌱
Lu et al. review the cysteine-rich peptides RALF and EPF/EPFL, and their roles in regulating growth, development, immune responses and stress adaptation, highlighting new mechanistic insights and challenges in peptide research.
🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪
Christoph Ringli and collaborators identify a new regulator of the Arabidopsis extensin-mediated cell wall integrity sensing: phosphatase PP2C12 inhibiting receptor kinase FERONIA
www.embopress.org/doi/full/10....
Could it be an American badger? Flat skull, long canine teeth, google images of badger skulls look quote similar...
Thrilled to share our latest review on receptor kinase processing in plants! 🌱
Huge congratulations to @adithya1972.bsky.social and Anna for leading this work, and a big thank you to our fantastic collaborator Martina-Ried Lasi for making this a truly rewarding team effort!
What... There is an Arabidopsis gene called Lord of the Rings (AT5G50150).
"LOTR1 protein has an unknown function. It contains both DUF4409 and DUF239 domains. Loss of function mutations show defects in formation of the Casparian band, which is correlated with mis-localization of CASP1. "
"Plants have a secret language that scientists are only now starting to decipher" - Nature News feature
@nature.com
www.nature.com/articles/d41...
Signals from the plant cell wall help to orchestrate growth, reproduction and immune function. Can we harness them to create better crops?
Fran @frangordilloc.bsky.social and I wrote this piece on one of our favorite topics: the evolutionary relevance of co-receptors for immune receptor transfer in #plants 🌱
#EvoMPMI #MPMI
Check also the beautiful paper by Zhang et al. on the PRR STOMR and discover what happened to its co-receptor 😱
A RALF-brassinosteroid signaling circuit regulates Arabidopsis hypocotyl cell shape doi.org/10.1016/j.cu...
Very proud to share our latest preprint: postdoc/LRSF fellow Yue Rui studies the mechanisms regulating attachment of the cell wall to the plasma membrane. The Cellulose Synthase Complex and REMORINs determine the extent of attachment and root osmotic stress tolerance. www.biorxiv.org/content/10.1...
One of the key aspects influencing morphogenesis is mechanics. We mapped the mechanical properties of living roots at the tissue and cellular levels using Brillouin microscopy and molecular rotors.
www.biorxiv.org/content/10.1...
Epidermal cells acquire different cell shapes to enable their functions and maintain tissue integrity in plants. Meristematic cells differentiate and expand into diverse mature cell types, including jigsaw puzzle-shaped pavement cells (a), round stomatal guard cells (b), elongated epidermal cells in hypocotyls and the apical hook (c), and root epidermal cells with root hairs (d). The spatial distribution and arrangement of cell wall polysaccharides, such as cellulose microfibrils, xyloglucans, and pectins (e.g., homogalacturonan represent as HG), and the abundance of methylester groups on these components in differentiating cells regulate anisotropy during cell growth, enabling the acquisition of specific cell shapes. In addition, proteins like KATANIN and CLASP reorient dynamically the cortical microtubules. These cortical microtubules rearrangement is in response to mechanical cues, either self-generated (as in b, c) or from neighboring cells (a, c), leading to the resulting cell shape. Attribution-NonCommercial-NoDerivatives 4.0 International
Auxin controls cell expansion by regulating cell wall biosynthesis and cell remodeling. Auxin promotes cell expansion by acidifying the cell wall and activating wall synthesis and loosening enzymes. Auxin efflux (PINs) and influx (AUX1) transporters establish concentration gradients in growing tissue. Auxin enters cells via influx transporters and activates the TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX PROTEINS-AUXIN/INDOLE ACETIC ACID (TIR1/AFB-Aux/IAA) nuclear signaling cascade, which regulates auxin-responsive genes, including AUXIN RESPONSE FACTORs (ARFs) and SMALL AUXIN UP RNAs (SAURs). Auxin activates the H+-ATPase proton pump through TRANSMEMBRANE KINASE 1 (TMK1), acidifying the cell wall and triggering loosening enzymes including PECTIN METHYLESTERASEs (PMEs), EXPANSINs, and XYLOGLUCAN:XYLOGLUCOSYL TRANSFERASEs (EXTs). Cellulose microfibrils are synthesized by the plasma membrane-bound cellulose synthase complex (CSC), with cortical microtubules guiding the exocytosis of this complex toward expanding cell edges. Auxin coordinates the reorientation of cortical microtubules and actin filaments to regulate the trafficking of cell wall polysaccharides to ensure proper cell wall expansion and specific cell shape acquisition. Attribution-NonCommercial-NoDerivatives 4.0 International
🌱🧩 How do plant cells get their shape? 🧪
Check out the new #open-access #review exploring the mechanochemical duet between auxin & the cell wall in shaping diverse plant cell types from @srobertgroup.bsky.social's Lab.
🔗 doi.org/10.1111/ppl....
#PlantBiology #CellShape #Auxin #Biomechanics
Infographic demonstrating how multifactorial stress influences cell wall integrity and cell wall-dependent pathogen susceptibility.
DARWIN REVIEW: "Plant cell wall structure and dynamics in plant–pathogen interactions and pathogen defence" - Kristina S Munzert et al, doi.org/10.1093/jxb/...
#plantscience 🧪
The FERONIA–RESPONSIVE TO DESSICATION 26 module regulates vascular immunity to Ralstonia solanacearum academic.oup.com/plcell/advan...
#PlantScience
