Galligan Lab

Awesome!!!!

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Glyoxalase 1 is a proadipogenic gene Diabetes is one of the most prevalent and widespread diseases, with the majority of cases stemming from prolonged obesity. Obesity occurs through the expansion of adipose tissue in an unhealthy and dy...

www.jbc.org/article/S002...

Profiling the proteome-wide selectivity of diverse electrophiles - Nature Chemistry Covalent inhibitors are powerful entities in drug discovery. Now the amino acid selectivity and reactivity of a diverse electrophile library have been assessed proteome-wide using an unbiased workflow...

How can we study target engagement and selectivity of covalent inhibitors? Which electrophilic probes are best suited to study a certain amino acid?

Our study on "Profiling the proteome-wide selectivity of diverse electrophiles" is published in Nature Chemistry.(1/7)

www.nature.com/articles/s41...

Glycation-lowering compounds curb hunger, lower insulin resistance and extend lifespan in mice Buck scientists fight the sugary chemistry of aging The same chemical reaction that makes a piece of freshly toasted bread delicious also happens in our bodies, with far less appetizing consequences. ...

Can a cocktail of glycation-lowering compounds curb #hunger, lower #insulin resistance and extend #lifespan? It does in mice! Buck scientists fight the sugary chemistry of #aging. www.buckinstitute.org/news/glycati... @cp-cellreports.bsky.social, @pkapahi.bsky.social

Reactivity-based metabolomics reveal cysteine has glyoxalase 1-like and glyoxalase 2-like activities - Nature Chemical Biology Using reactivity-based metabolomics, methylglyoxal and its main metabolite lactoylglutathione were found to react rapidly with cysteines to generate l-lactoylated and d-lactoylated thioesters, suggest...

A new paper used reactivity-based metabolomics to show that methylglyoxal and its main metabolite lactoylglutathione react rapidly with cysteines to generate L- and D-lactoylated thioesters suggesting an additional pathway to enable protein lactoylation

www.nature.com/articles/s41...

Sorbate induces lysine sorbylation through noncanonical activities of class I HDACs to regulate the expression of inflammation genes Sorbic acid regulates inflammatory gene expression through the mechanism of lysine sorbylation.

Super excited to share our new publication identifying the food preservative, sorbic acid, as a new PTM. Even cooler is that the deacylation and acylation of this PTM is catalyzed by the non-canonical activity of Class I HDACs using free sorbate (not sorbyl-CoA) www.science.org/doi/10.1126/...

Reactivity-based metabolomics reveal cysteine has glyoxalase 1-like and glyoxalase 2-like activities - Nature Chemical Biology Using reactivity-based metabolomics, methylglyoxal and its main metabolite lactoylglutathione were found to react rapidly with cysteines to generate l-lactoylated and d-lactoylated thioesters, suggest...

www.nature.com/articles/s41...

Transfer RNA acetylation regulates in vivo mammalian stress signaling An ancient tRNA modification is used by mammalian cells to coordinate protein translation and adaptive signaling.

Excited to share this one! We developed an in vivo model for specific manipulation of transfer RNA acetylation and found it serves as a sentinel modification whose loss causes ribosome stalling and stress signaling. Implications for a genetic disorder and cancer.

www.science.org/doi/10.1126/...

Woooooo!!!!!!!

A nucleosome switch primes hepatitis B virus infection A reconstituted cccDNA platform facilitated the discovery of an early chromatinization event that promotes transcription of the hepatitis B virus (HBV) regulatory X gene. The use of a small-molecule c...

I am extremely proud to see our work about Hepatitis B now published in @cellpress.bsky.social

From basic research to identifying the key step of infection & a potential first drug for curing this devastating disease. This has been an incredible journey.🧵

www.cell.com/cell/fulltex...

Coenzyme A biosynthesis: mechanisms of regulation, function and disease - Nature Metabolism This Review summarizes the fundamental aspects related to coenzyme A synthesis and its implications as a central molecule in metabolism.

First post! Starting by pinning our review in Nature Metabolism on Coenzyme A biosynthesis and metabolism. Check it out if you didn't catch it when it was published!

www.nature.com/articles/s42...

SLC7A5 is required for cancer cell growth under arginine-limited conditions Metabolic adaptations are key to cancer cell survival in nutrient-limited microenvironments, but strategies for coping with arginine scarcity are poorly understood. Dunlap et al. use CRISPR screening and metabolomics to identify the plasma membrane transporter SLC7A5 as a citrulline transporter essential for cancer cell growth under arginine limitation.

Happy New Year. I’m pleased to report publication of the Ducker lab’s latest cancer metabolism paper led by graduate student Kyle Dunlap in @cp-cellreports.bsky.social

www.cell.com/cell-reports.... 1/n

31 grants!?

Hoping this is like what the old one used to be!