Hi microscopy folks! I'm looking for your advice 💡
We're shopping for cyclic immunofluorescence instruments to increase throughput and multiplex-ability of tissue IF. Have you worked with cyclic IF platforms (Comet, CellScape, Cell DIVE)? Thoughts? Pain points? Alternatives to consider? Thanks!
I've spent many afternoons crouched over a microscope with a pipette, waiting for the exact right moment to add my perturbation (& not breathing so I don't bump anything). Now I can just program it in and have a more relaxing experiment :)
The official deadline is February 1, 2026, but we'll start reading applications as they come in. To be eligible, you must be currently enrolled in a PhD program at a US university (sorry, awesome international microscopists).
I'm excited to be hiring an intern to develop new fluorescence-based assays for alpha synuclein aggregation at Calico during summer 2026 😃 🔬
If you're a current PhD student who loves microscopy, unnatural amino acids, and/or synuclein biophysics, please apply!
www.calicolabs.com/careers/?gh_...
If you want to read more, please check out the full paper here: andrewgyork.github.io/tumbling_is_...
We think it will work with most fluorescent proteins and (relatively) minimal modification of existing instruments
Data from an unmodified commercial confocal instrument that we jerry-rigged into taking tumbling measurements. A proof of principle & far from what you could do with a custom rig!
Want to measure protein-protein interactions? We recently did some dreaming about how you can do this with a single fluorescent tag using molecular "tumbling." We simulated how you'd do this on a lightsheet, a flow cytometer, or a cheap handheld device. We even took prelim data on a commercial rig:
I no longer have the follower count nor the algorithm to help promote stuff, but @jlazzaridean.bsky.social and Andy York reached out to me a while ago to do an animation explaining a way to measure moluecular "tumbling" and I think it worked out pretty well! andrewgyork.github.io/tumbling_is_...
Hi bluesky! Excited to share some new work in voltage sensing I was a part of in my past life in the Evan Miller lab at Berkeley: a versatile dye for both fast voltage imaging and lifetime-based quantification
Introducing CRhOMe: www.biorxiv.org/content/10.1...
