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Leon-Samuel Icking

@lsi

PhD student from the Kalmbach Lab in NeuchΓ’tel flowerpunk 🏡️ + solarpunk β˜€ Anything SynBio (and Plants πŸ‘€)! iGEM Freiburg Ultra🀘 Chaotic neutral

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04.09.2023
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Latest posts by Leon-Samuel Icking @lsi

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Quantitative imaging of apoplastic pH in plant roots via confocal microscopy Nature Protocols, Published online: 11 March 2026; doi:10.1038/s41596-026-01337-xThis protocol details the quantitative imaging of apoplastic pH in Arabidopsis thaliana roots using confocal microscopy combined with the fluorescent pH probe 8-hydroxy-pyrene-1,3,6-trisulfonic acid trisodium salt, enabling apoplastic pH dynamics to be assessed with high spatial resolution and accuracy.

New Article! Quantitative imaging of apoplastic pH in plant roots via confocal microscopy

11.03.2026 18:03 πŸ‘ 8 πŸ” 4 πŸ’¬ 1 πŸ“Œ 0
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Something exciting for student across Africa.
@Synbio4allA (SynBio4ALL) Lil Lab Innovation Program Micro-Grant 2026 is coming.
β€’ $50 micro-grant
β€’ Mentorship
β€’ Chance for a $100 follow-up grant
6 student teams from Africa.
Applications opening soon
Follow SynBio4ALL Africa.

12.03.2026 17:47 πŸ‘ 3 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0

You can @ her
@yschaerli.bsky.social

12.03.2026 09:39 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Quantitative imaging of apoplastic pH in plant roots via confocal microscopy Nature Protocols - This protocol details the quantitative imaging of apoplastic pH in Arabidopsis thaliana roots using confocal microscopy combined with the fluorescent pH probe...

Two papers on apoplastic pH visualisation coming out together – a nice double pack from @aroessling.bsky.social and @elkebarbez.bsky.social

-> www.nature.com/articles/s41...
-> link.springer.com/article/10.1...

@dompsfr.bsky.social @biologyunifreiburg.bsky.social @cibss.bsky.social

11.03.2026 18:00 πŸ‘ 34 πŸ” 20 πŸ’¬ 1 πŸ“Œ 0
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Want to image apoplastic pH in plants? πŸ”¬

Two new papers from our team offer a full workflow plus open access analysis tools.
Congrats to @aroessling.bsky.social, Niklas Mayle and @lguerard.bsky.social

Nature Protocols: rdcu.be/e7R2E
BMC Methods: lnkd.in/dctpWJ3X

Happy Imaging!

11.03.2026 19:59 πŸ‘ 50 πŸ” 28 πŸ’¬ 0 πŸ“Œ 2
TSL Summer Conference in Plant-Microbe Interactions, 20th – 31st July… Inviting Early Career Researchers to join international experts in discussing the latest approaches and discoveries in Plant-Microbe Interactions …

If you have the opportunity, apply for the TSL @thesainsburylab.bsky.social Summer Conference. An intense week with top experts in plant-microbe interactions. What you can learn here in a week would normally take months (or longer)!!! @kamounlab.bsky.social πŸ¦ πŸ”¬πŸŒ±

www.tsl.ac.uk/tsl-summer-c...

09.03.2026 11:01 πŸ‘ 8 πŸ” 8 πŸ’¬ 0 πŸ“Œ 0
AlphaFold 3 From Scratch in Pytorch - Introduction and Setup
AlphaFold 3 From Scratch in Pytorch - Introduction and Setup YouTube video by Kilian Mandon

Kilian is just crazy...

www.youtube.com/watch?v=CTlk...

10.03.2026 20:24 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

#Chemchat, take a look at this.

10.03.2026 11:06 πŸ‘ 18 πŸ” 5 πŸ’¬ 3 πŸ“Œ 0
Spectral properties of the Zinc sensor

Spectral properties of the Zinc sensor

An Ultraresponsive Green Biosensor for Robust in Vivo Imaging of Synaptic Zinc Dynamics
pubs.acs.org/doi/full/10....

07.03.2026 21:10 πŸ‘ 21 πŸ” 6 πŸ’¬ 0 πŸ“Œ 0

The science is as over-hyped and borderline fraudulent as the ethics are thorny (and that doesn't even count the George Church, friend of Epstein angle)

05.03.2026 17:53 πŸ‘ 42 πŸ” 14 πŸ’¬ 6 πŸ“Œ 1

Oh no what did BC do...

05.03.2026 18:04 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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🧬 Just pushed new updates to #OpenCloning Assembler based on user feedback!

✍️ Now supports #GoldenBraid and Plant #MoClo

πŸš€ You can edit assembly product names, view a full syntax overview, and enjoy cleaner visuals.

πŸ‘‰ Try it out (link in comments) β€” feedback welcome!

04.03.2026 16:46 πŸ‘ 6 πŸ” 1 πŸ’¬ 2 πŸ“Œ 0
Wave-like contamination on a 1/2 MS plate

Wave-like contamination on a 1/2 MS plate

@contamclub.bsky.social

03.03.2026 08:25 πŸ‘ 3 πŸ” 1 πŸ’¬ 1 πŸ“Œ 0

Since people are liking the Bocchi colors, I have a whole collection of color palettes on my GitHub: github.com/cxli233/Colo...

You will find the hex code, the preview, and the inspiration for each color palette. I hope some of them will be used.

#DataVis

28.02.2026 20:55 πŸ‘ 43 πŸ” 10 πŸ’¬ 1 πŸ“Œ 0
Addgene: OpenPlant Kit Use the OpenPlant toolkit for genetic engineering of the plant synthetic biology model Marchantia polymorpha.

The OpenPlant kit is finally available from Addgene as... a kit :)
www.addgene.org/kits/haselof...
@addgene.bsky.social #marchantia #synbio

26.02.2026 01:08 πŸ‘ 25 πŸ” 10 πŸ’¬ 0 πŸ“Œ 1
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Unmasking Academe’s Gilded Boys’ Club The Epstein files reveal an elite, chummy, and venal scholarly network.

β€œ...the old conferences did not care about diversity. I suggest you not either,” Epstein wrote to Brockman. β€œThe women are all weak, and a distraction sorry.” πŸ˜‘πŸ‘©πŸ»β€πŸ”¬ #AcademicSky

26.02.2026 16:54 πŸ‘ 13 πŸ” 7 πŸ’¬ 2 πŸ“Œ 0

New Ed Yong book in April 2027 let's gooooo

25.02.2026 22:04 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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We teamed up with OpenFlower (@nickdesnoyer.bsky.social), a nonprofit creating designer flowers for art & education, to release 3 limited edition postcards! These flowers were powered by Whole Plasmid Sequencing from Plasmidsaurus.

Want one? Just fill out the form!
tinyurl.com/plasmidsauru...

23.02.2026 16:43 πŸ‘ 15 πŸ” 7 πŸ’¬ 0 πŸ“Œ 2
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πŸš€ Excited to launch the #OpenCloning Assembler!

🧬 Built to demystify Golden Gate for beginners and save time for experts -> MoClo assembly blazing fast ⚑

✍️ Use existing syntaxes or design your own!

#SynBio #GoldenGate #MoClo #OpenSource #Biotech

πŸ‘‰ Try it: app.opencloning.org

Feedback welcome!

20.02.2026 12:54 πŸ‘ 32 πŸ” 14 πŸ’¬ 4 πŸ“Œ 4
Dennis nedry jurrasic park "see nobody cares" meme reads

Hey everybody, this guy still posts on X!

See? Everyone is horrified and disappointed. They feel it speaks directly to your values.

Dennis nedry jurrasic park "see nobody cares" meme reads Hey everybody, this guy still posts on X! See? Everyone is horrified and disappointed. They feel it speaks directly to your values.

18.02.2026 20:13 πŸ‘ 18817 πŸ” 3704 πŸ’¬ 150 πŸ“Œ 30
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The mechanical properties of Arabidopsis thaliana roots adapt dynamically during development and to stress Brillouin microscopy reveals in vivo dynamics of mechanical properties during plant development and response to stress.

Our work now on its final version. We mapped the mechanical properties of roots at tissue and single cell levels using Brillouin microscopy and molecular rotors. Additional mutants and stress measurements from what we previously showed in the preprint are included.
www.science.org/doi/10.1126/...

18.02.2026 20:22 πŸ‘ 58 πŸ” 32 πŸ’¬ 5 πŸ“Œ 3

DeepL seems to have updated and has removed the "thesaurus" feature. Does anybody know a good alternative to DeepL?

19.02.2026 10:19 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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A dual role of Arabidopsis PAL nuclear localization in fine tuning flavonoid biosynthesis PAL’s dual localization links metabolism with transcription, revealing feedback that fine-tunes phenylpropanoid regulation.

Interesting paper: Metabolic enzyme PAL can go into the nucleus in response to high product build up and sequester an activating TF of the pathway, thus acting as a feedback inhibition loop.

From: www.science.org/doi/full/10....
#PlantScience

18.02.2026 13:04 πŸ‘ 17 πŸ” 13 πŸ’¬ 0 πŸ“Œ 0
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Very nice genetic dissection of the roles of BSK kinases in brassinosteroid signaling by Wenqiang Tang's lab. bsk-u (ko for BSK1 1, 2, 3, 4, 5, 6, 7, 8, 10, 11 & 12) really looks like a bri1 null mutant and can be rescued by over-expressing BSK3. nph.onlinelibrary.wiley.com/doi/epdf/10....

17.02.2026 13:42 πŸ‘ 16 πŸ” 7 πŸ’¬ 0 πŸ“Œ 0
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iGEM Registry The iGEM Registry is a community-curated collection of standardised genetic parts used to design, build and share synthetic biology devices and systems.

Oops, I think this part got all the documentation: registry.igem.org/parts/bba-25.... Or ask Nicole, she'll definetly know

16.02.2026 21:24 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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iGEM Registry The iGEM Registry is a community-curated collection of standardised genetic parts used to design, build and share synthetic biology devices and systems.

You could try the one the iGEM team made this year (registry.igem.org/parts/bba-25...), should still be in the Signalhaus. to use it it not as an intermedia but rather as primary, you just have to change the epitope I guess πŸ‘

16.02.2026 21:12 πŸ‘ 0 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

And save >80% on the cost by pooling 6 or more plasmids into a single sample using our free SAVEMONEY algorithm!

elifesciences.org/articles/88794

colab.research.google.com/github/Masaa...

15.02.2026 03:04 πŸ‘ 101 πŸ” 34 πŸ’¬ 8 πŸ“Œ 2

Transcription terminators can be strong promoters https://www.biorxiv.org/content/10.64898/2026.02.12.705533v1

14.02.2026 10:21 πŸ‘ 1 πŸ” 1 πŸ’¬ 0 πŸ“Œ 0

Thanks Rita, it's not urgent :)

14.02.2026 12:12 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0