โจ Blinking #nanobodies that work for single-molecule localization ๐ฌ
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
PREPRINT OUT โจ "hack" your microscope to work as a 3D printer! print micrometer sized features at cm scale. a method for easy and cheap microfabrication, with a focus on biological applications. THREAD โ
www.biorxiv.org/content/10.1...
YES! THIS on GenAI!
Please read this absolutely splendid piece of writing that had me cheering, a little bit weepy, and writing in the margins:
"An extraordinary amount of money is spent by the AI industry to ensure that acquiescence is the only plausible response. But marketing is not destiny."
Not only is the ratio of Al's resource rapacity to its productive utility indefensibly and irremediably skewed, Al-made material is itself a waste product: flimsy, shoddy, disposable, a single-use plastic of the mind.
I also loved this observation. The techโs true โinnovationโ is its utter disregard for efficiency. It โworksโ bc it accepts the most irresponsibly resource-intensive production method imaginable. What matters is not what it costs but *who* pays. The apotheosis of a culture built around disposability
Save the date! The @pasteur.fr Pasteur course on image analysis is announced:
It will happen from the 1st of June to the 5th 2026.
This year there will be only one track: the Early Career Investigator track (beginners). ๐
Just posted (bioRxiv): A novel #mechanobiology model revealing how #immune #Tcells combine a #nucleus as a #piston, an #uropod, and #microenvironment to power #amoeboid #cellmigration in #cellconfinement via a hydraulic cell engine. #CellBiophysics #ImmuneMigration
www.biorxiv.org/content/10.1...
"This could be the difference whether your child gets cancer. Are you willing for them to die because the therapy for them is delayed?"
Powerful video with voices from top US scientists on the very real and devastating impacts of the funding cuts.
Enough is enough. The health of the country is in danger. Read our resignation letters: insidemedicine.substack.com/p/breaking-n...
Final call: Applications close September 2! KI is recruiting 20 Assistant Professors in a wide range of subject areas. We're looking for early-career researchers with strong scientific merits and future potential. All positions: ki.se/en/about-ki/...
Destroying the agency that brought us the internet, the SuperCollider and modern telescopes. Heading into the dark ages here
If you're in the US, considering Europe for your PhD, and you identify as a minority, I will chat with you and review any application documents you need ๐งช
Wondering if your study section cancelled? I update this sheet daily. As of today, 56/124 study sections that should have met since Jan 2, 2025 have "not met as scheduled." docs.google.com/spreadsheets...
The US plans to cut billions in reimbursements to medical researchers for items such as hazardous waste disposal.
Fig. 1 Photostable and fluorescence properties of mScarlet3-S2. a, b, Photostability of red emitting mScarlet variants expressed in live U-2 OS cells under continuous WF illumination at 1.9 W/cm2
A highly photostable monomeric red fluorescent protein
www.researchsquare.com/article/rs-5...
UCLA QCBIO is looking for its next director! Please consider applying! recruit.apo.ucla.edu/JPF10007
International Microscopy Facility Survey - the survey includes questions on Optical Microscopy, Electron Microscopy and Data Analysis facility operations.
Please check the questions before starting the survey (lnkd.in/giJApzUa).
The survey will close on Friday 20th December 2024: lnkd.in/gNxz9uA3
After 1.5 years of tinkering and three rewrites, I'm happy to say that my web-based ray tracer for #optics and lens design has reached a point where I can finally start packing in features and make it *useful.*
Feel free to let me know what you think.
kmdouglass.github.io/cherry/
We are happy to report that our journals and community sites now have Bluesky accounts. Follow them at:
@dev-journal.bsky.social
@jcellsci.bsky.social
@jexpbiol.bsky.social
@dmmjournal.bsky.social
@biologyopen.bsky.social
@the-node.bsky.social
@prelights.bsky.social
@focalplane.bsky.social
Hi NIH grantees - would you request NIBIB these days as primary institute give wnat is coming towards us in January? Pay line is higher - but the institute might not exist any more in February. NCI pay line is lower but seems safer.๐ค
This is a beautiful field of view! ๐คฉ
๐ฌCurious about what makes my role as an ๐ฅ&๐ ๐ฆ๐ฐ๐ถ๐ฒ๐ป๐๐ถ๐๐ ๐ถ๐ป ๐ข๐ฝ๐๐ถ๐ฐ๐ฎ๐น ๐ ๐ฎ๐ป๐๐ณ๐ฎ๐ฐ๐๐๐ฟ๐ถ๐ป๐ด at ZEISS Group so exciting? Letโs dive into it... ๐ ulrikeboehm.org/what-rd-scie...
the vasculature of the murine retina, depth coded by color.
For #fluorescencefriday, the vasculature of the mouse retina perfused with far red fluorescent lectin and cleared with EZClear from:
elifesciences.org/articles/77419
star-protocols.cell.com/protocols/3458
It's not a repost if it's a new platform, right? right??
Lattice light sheet movie of actin in mitosis. Blebby.
Timelapse SIM of Bacillus subtilis MreB-HaloTag (40x actual speed)
Hi everyone! We study biophysics of bacterial cell wall division and growth using advanced light microscopy holdenlab.github.io #microsky #scisky #microscopy
๐๐ง Bluesky Intro please repost๐: Welcome to the Solecki Lab! We dive into how young nerve cells navigate & mature, building brain circuits that shape thought. Follow along for behind-the-scenes updates & mesmerizing microscopy videos, captured by me. You wonโt want to miss what we uncover nextโฆ
Reminder for newcomers that bioRxiv has Bluesky accounts in every subject category - great way to keep up (please re-skeet) connect.biorxiv.org/news/2023/09...
And you have all the rights to be super proud of it! Itโs a really nice cover! ๐คฉ
Thank you!
Hi Mike, great list, thank you! I would love to be added: I'm the CMB microscopy core director at St. Jude Children's Research Hospital. ๐ฌ
