Mathias Nielsen

Tripartite phosphorylation of SPT5 by CDK9 times pause release and tunes elongation rate of RNA polymerase II Sun and Fisher performed chemical-genetic dissection of the conserved transcription elongation factor SPT5 in human cells, which revealed that CDK9 phosphorylates three distinct regions in SPT5 that coordinately regulate RNA polymerase II pausing, elongation rate, pre-mRNA splicing, and termination to ensure productive transcription and cell viability.

Online Now: Tripartite phosphorylation of SPT5 by CDK9 times pause release and tunes elongation rate of RNA polymerase II Online now:

New Article: "H3K36 methylation stamps transcription resistive to preserve development in plants" rdcu.be/efNVu

New chromatin state, transcription resistive, different from activation & silencing, marked by H3K36me, needs interplay between SDG8 and other H3K36me MTs.

Valentine's Dino-grams for you, your lab mates, and your loved ones! 💖 Print and share all of them here: plasmidsaurus.com/o/valentines... (2/2)

VACANCY - Are you a molecular biologist and looking for a postdoc?

Join Peter Kindgren's group @umeaplantscicentre.bsky.social and generate superior plants in terms of biomass and stress resilience.

Read more here and apply:
slu.se/en/about-slu...

#PlantSciJobs #PlantSciJob #hiring #plantscience

UK is cold 🥶

The best thing about FACS sorting is that it provides the best time for reading.

Enhancer cooperativity can compensate for loss of activity over large genomic distances Thomas, Feng, et al. introduce a synthetic platform that allows the building of complex regulatory landscapes. Integrating the same enhancer at different distances from a promoter uncovered that activ...

Enhancer cooperativity can compensate for loss of activity over large genomic distances www.cell.com/molecular-ce...

a group of bald men are sitting in a stadium with their hands in the air . ALT: a group of bald men are sitting in a stadium with their hands in the air .

After waiting six weeks, my reagent has finally arrived.

Rapid folding of nascent RNA regulates eukaryotic RNA biogenesis An RNA′s catalytic, regulatory, or coding potential depends on RNA structure formation. Because base pairing occurs during transcription, early structural states can govern RNA processing events and d...

We are happy to share the development of Co-transcriptional Structure Tracking by @leoschaerfen.bsky.social, which measures RNA structure formation during its synthesis in vivo. We are excited to discover the activity of helicases on nascent rRNA!
www.biorxiv.org/content/10.1...

ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene regulatory elements - Nature Genetics ChIP-DIP (ChIP done in parallel) is a highly multiplex assay for protein–DNA binding, scalable to hundreds of proteins including modified histones, chromatin regulators and transcription factors, offe...

Gene regulation involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. Our paper in Nature Genetics describes ChIP-DIP, a method for genome-wide mapping of hundreds of DNA-protein interactions in a single experiment.
www.nature.com/articles/s41...

A quantitative framework for structural interpretation of DMS reactivity Dimethyl sulfate (DMS) chemical mapping is widely used for probing RNA structure, with low reactivity interpreted as Watson-Crick (WC) base pairs and high reactivity as unpaired nucleotides. Despite i...

🧬 Excited to share our new preprint! DMS chemical mapping, a key technique for studying RNA structure. Everyone assumes low DMS reactivity = Watson-Crick , high = non-WC. However, analyzing 7,500 RNA structures containing known 3D structures reveals it's not that simple. doi.org/10.1101/2024...

November 18 is the International Day of LGBQTIA+ People in STEM! Today more than ever we want to stress the importance of communities, building connections, and raising each other up. Let's keep leaving our mark for the betterment of us all! 🏳️‍⚧️🏳️‍🌈 #QueerinSTEM

prideinstem.org/lgbtstemday/

A lab question for scientists in #PlantScience #RNAsky #ScienceSky 🧪🧬:
What is your favorite RNase inhibitor for plant RNAses? More precisely it's for young tomato 🍅 fruits.
#RNA #RNase
Thank you 🙏🙏

I just finished baking, and now it's time for a run. It's the perfect way to relax on a Saturday!

Failing to account for RNA quantity inflates background and leads to the misleading appearance that PRC2 and GFP bind to RNA in vivo We recently published biochemical and quantitative evidence that challenges the widespread claims that PRC2 binds directly to many RNAs in vivo . A recent preprint performs a re-analysis of some of ou...

The saga continues:

Does PRC2 binds RNA or not?

www.biorxiv.org/content/10.1...