Sebastian Schade

bioRxiv: Plasma membrane nanoscale dynamics of Arabidopsis leucine-rich repeat receptor kinase complexes (2026) Plasma membrane-localized receptors operate as dynamic signaling complexes and integrative networks, yet the spatial and temporal regulation of these interactions remain largely unknown. Here, by analyzing the components of a minimal Arabidopsis leucine-rich repeat receptor kinase network, we describe the differential diffusion and organization of receptor complex components and unveil the nanoscale spatial and temporal logic underlying the formation of receptor kinase complexes. The ligand-binding receptors FLS2 and BRI1, and the accessory receptor BIR3, are organized in plasma membrane nanodomains, within which the co-receptor BAK1 diffuses and is spatially arrested upon ligand perception. BAK1 spatial arrest relies on extracellular domain (ECD)-ECD interactions but does not require receptor complex activation. Mathematical modelling, single molecule imaging and bio-assays infer that accessory receptors maintain a dynamic pool of co-receptors in the vicinity of ligand-binding receptors to promote ligand-induced complex formation and signaling. We propose that ligand-induced receptor kinase complex formation is a deterministic process defined by the relative nanoscale spatial positioning of individual signaling and regulatory components.

New Preprint: Plasma membrane nanoscale dynamics of Arabidopsis leucine-rich repeat receptor kinase complexes (2026)

https://www.tsl.ac.uk/publications/164874

The Receptor Kinase MEE39/ATHE Mediates Cell Wall Integrity Surveillance During Root Vascular Pathogen Infection https://www.biorxiv.org/content/10.64898/2026.03.05.708959v1

Alternative splicing expands the functional portfolio of a plant virus to control the viral cycle https://www.biorxiv.org/content/10.64898/2026.02.19.706752v1

Very nice and useful.
onlinelibrary.wiley.com/share/F87AVP...

Dear PPRM community, we are pleased to invite you to the next PPRM meeting, 14-16 September 2026 in Freising, Germany.

Registration opening soon!

Plants use cell-surface and intracellular receptors that collaborate to detect pathogens🦠. We discovered that a key #ubiquitin recognition event recruits both receptor types into an unexpected dual receptor complex that boost the translation of defence proteins and establishes robust #PlantImmunity👇🏾

Please share!
My group at @zmbp-tuebingen.bsky.social is offering a post-doctoral position (4 years). We look for a structural biologist with experience in Cryo-EM/Cryo-ET to investigate the mechanisms of host invasion by pathogenic fungi. Deadline February 28th!
uni-tuebingen.de/universitaet...

Diversify and conquer: How effector diversity is shaped by host–microbe co-evolution

Thank you
@marioncmueller.bsky.social, @binebrumm.bsky.social, Eric Kemen, Yiheng Hu and Thomas Lahaye for this nice common effort on effector diversity.

Diversify and conquer: How effector diversity is shaped by host–microbe co-evolution
doi.org/10.1371/jour...

Important theoretical framework for NLR evolution.
doi.org/10.32942/X2R...

Membrane-binding domains define REMORIN phylogeny and provide a predicted structural basis for distinctive membrane nano-environments https://www.biorxiv.org/content/10.64898/2025.12.22.695504v1

Manipulation of REMORIN gene dosage affects salt signaling and tolerance in Arabidopsis thaliana https://www.biorxiv.org/content/10.64898/2025.12.22.695520v1

An ancient alkalinization factor informs Arabidopsis root development https://www.biorxiv.org/content/10.64898/2025.12.22.695669v1

Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n

Queer in Microbiology: A Conversation 18 Nov - Virtual, online

It’s #TransAwarenessWeek 🌈
This week, we’re celebrating trans and nonbinary people while also raising awareness of the challenges and discrimination that many still face. Check out our discussion with Rowan Casey: microb.io/4hFL6vk 👈
#TransInSTEM #TransInScience #LGBTQSTEMDay

Thanks for their contribution to: Henriette Leicher, Martin Stegmann, Michelle von Arx, @isabelmonte.bsky.social, @julien-gronnier.bsky.social

The interplay of RALF structural and signaling functions in plant-microbe interactions Rapid alkalinization factor (RALF) peptides are important signaling molecules binding to Catharanthus roseus receptor-like kinase 1-like (CrRLK1L)—LORELEI/LORELEI-like GPI-anchored protein complexes t...

Our Mini-review is out now! Here we collect recent findings of RALF peptide functions, adress their (potential) roles in plant-microbe interactions and discuss the resulting emerging questions. Pls share!

dx.plos.org/10.1371/jour...

Splicing was discovered in adenoviruses, but was thought to be only anecdotal in plant viruses... We may need to revisit plant viral transcriptomes and proteomes!

Pervasive splicing in a plant DNA virus Viruses maximize their limited coding space through strategies that increase transcript and protein diversity. In mammalian viruses, splicing is a well-established mechanism for proteome expansion, ye...

How do geminiviruses maximize their limited coding capacity? Our recent preprint uncovers splicing of viral transcripts as one more strategy used by this viral family. We show that RNA splicing is prevalent in the geminivirus TYLCV — and required for infectivity! www.biorxiv.org/content/10.1...

Very excited to share that our paper "A RALF-brassinosteorid signaling circuit regulates Arabidopsis hypocotyl cell shape" is now published in @currentbiology.bsky.social !

Enjoy reading it!!

www.sciencedirect.com/science/arti...

A RALF-brassinosteroid signaling circuit regulates Arabidopsis hypocotyl cell shape doi.org/10.1016/j.cu...

📢 A Junior Professorship (W1, tenure track) is open at the Center for Plant Molecular Biology at Uni Tübingen (@zmbp-tuebingen.bsky.social) — to work on plant protein biochemistry (composition, structure, dynamics, or regulation of protein complexes). 🌱 Great opportunity — Apply by Oct 17th!

CEPR2 perceives group II CEPs to regulate cell surface receptor-mediated immunity in Arabidopsis Author summary To protect themselves against pathogens, plants have evolved a sophisticated and intricate immune system that allows pathogen detection and the activation of appropriate defense respons...

CEPR2 perceives group II CEPs to regulate cell surface receptor-mediated immunity in Arabidopsis doi.org/10.1371/jour...