Joshua Saldivar

Engineering intrinsically disordered regions for guiding genome navigation Liu et al. design and test synthetic IDRs based on simple design rules derived from mutation-based analysis of 25 native TF IDRs. They show that hydrophobic amino acids endow activity, acidic ones prevent autoinhibition, and hydrophilic ones can tune specificity.

Engineering intrinsically disordered regions for guiding genome navigation

Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stability Marmolejo et al. uncover transcription condensate dynamics at histone locus bodies across the cell cycle. These dynamics are governed by cell cycle (DDK and CDK1/2) and checkpoint (ATR-CHK1) kinases to precisely control the “on/off” of histone gene transcription and maintain genome integrity.

Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stability

Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stability Marmolejo et al. uncover transcription condensate dynamics at histone locus bodies across the cell cycle. These dynamics are governed by cell cycle (DDK and CDK1/2) and checkpoint (ATR-CHK1) kinases t...

I am excited to share the first paper from the Saldivar lab! A special thank you to all who contributed.

Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stability: Molecular Cell www.cell.com/molecular-ce...

PAF15–PCNA exhaustion governs the strand-specific control of DNA replication Nature - PCNA–PAF15 has a key role in determining replisome dynamics during genome replication and protecting against genome instability.

Dear Colleagues,

Delighted to share that our first lab paper is now published in Nature.!!! 😊😊😊

We identify a previously concealed “safety limit” in DNA replication program: cells intrinsically rate-limit PCNA via PAF15, shaping strand-specific and global replication dynamics.

👉 rdcu.be/e1bBt

I am recruiting postdoctoral fellows to develop new research directions in the lab. We study transcriptional regulation through biomolecular condensates in development and disease, with an emphasis on first-principles and mechanistic discovery. Please repost or share with colleagues.