Eliver Ghosn

To strengthen the European research on early life immunology we have started the EFIS study ELINE
Info and seminar announcement 👇🏼
#EFIS#ELINE#immunology

On the balance of knowledge - Nature Reviews Immunology Ruslan Medzhitov shares his thoughts on the balance between generating data and developing theories in immunology, with a focus on exploring the rules that govern complex systems.

Too much data, too little thinking.
A important essay from Ruslan Medzhitov on the importance of understanding data, not just generating it. A must read.
@Yale
www.nature.com/articles/s41...

The Yewdell Lab at Genentech is seeking a summer intern for 2026!! An excellent opportunity to learn how we combine fundamental, basic research with drug discovery to create novel therapeutics, and to see what biotech is all about. Apply now! roche.wd3.myworkdayjobs.com/ROG-A2O-GENE...

Download our large database of postdoc fellowships in all fields of research.

Database freely available to all; 281 fellowships.

Download here: research.jhu.edu/rdt/funding-...

Farm exposure in infancy is associated with elevated systemic IgG4, mucosal IgA responses, and lower incidence of food allergy Infants from a traditional farming community exhibited antibody profiles that were found to be associated with lower incidence of egg allergy.

Researchers in #ScienceTranslationalMedicine find a distinct #Bcell and antibody profile in infants and mothers in an Older Order Mennonite farming community, associated with lower incidence of egg #allergy. https://scim.ag/48rPBGS

PD-L2 Regulates Natural Antibody and IL-10 Secretion by B-1 cells B-1 cells are innate-like lymphocytes that play a critical role in homeostasis by secreting natural antibodies, typically IgM, and immunosuppressive molecules such as IL-10. However, the regulation of...

This #Preprint reports that B6 (but not wild mice) have a truncated PDL2, missing an active intracellular signalling domain:
"Surprisingly, in C57BL/6J (B6) mice PD-L2 has only a 5-amino acid intracellular domain, and the same is true for several other laboratory mouse strains"
#Immunology

$15k stipend to support 9 weeks of full time summer research!

For rising juniors/seniors who are able to travel to and obtain housing at the location of the lab they are placed in!

I did an HHMI summer experience in 2013 and it changed everything for me! Apply…or tell someone to apply!

🧪🧠

FlowLITE: A protocol to characterize and quantify total antibody isotypes in human plasma using flow cytometry FlowLITE is a flow cytometry bead-based assay that enables multiplexing and high-throughput quantification of all antibody isotypes from humans, mice, or other species, using small volumes of any fluid specimen. Here, we detail a FlowLITE protocol to quantify and characterize antibody light-chain and isotype expression in human plasma. We describe steps for titrating the capture and reveal antibodies, multiplexing assay beads, and staining samples. We then describe proced...

FlowLITE: A protocol to characterize and quantify total antibody isotypes in human plasma using flow cytometry #protocol #starprotocols #cellpress

#FlowLITE is a modular, easy-to-build, bead-based assay for characterizing & quantifying absolute number of antibodies in small volumes (~10μL) of any fluid specimen. This is Justin Hahm’s Honors Thesis project (Highest Honors!). He was mentored by PhD student Alex Nazzari, the co-first author.
2/4

Cell Press: STAR Protocols STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

While #FlowLITE quantifies total antibodies, #FlowBEAT also quantifies antibody specificities to defined antigens. Both protocols can be adapted to your research needs.
4/4
star-protocols.cell.com/protocols/4572

Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19 Nasal IgA1 autoantibodies against IFN-α are associated with better COVID-19 prognosis, including fewer symptoms and robust anti–SARS-CoV-2 immunity.

For context: FlowLITE was based on our previous technology, FlowBEAT, which we developed to measure human antibody isotypes against #SARSCoV2 and autoantigens in the #nasal #mucosa of #COVID19 patients (Sci Transl Med, 2024)
3/4
www.science.org/doi/10.1126/...

No more #ELISA assays?

Our 🌈 Annual Cook-off event keeps growing! We build community around great food, drinks, and music. Thank you for the delicious dishes and fantastic company.

The 2025 Chef Laureate goes to...

You will identify immune mechanisms underlying neuroinflammatory autoimmune diseases in children vs adults--autoimmune encephalitis (AE) and multiple sclerosis (MS). You will apply multi-omics single-cell assays to study the role of B/plasma cells and antibodies using patient samples (CSF and blood)

We are #hiring a postdoctoral fellow! Please share!

Join our B-cell team @Emory to lead NIH-funded projects on human neuro-immunology and autoimmunity in a co-mentorship and collaboration with neuroscientist Dr. Grace Gombolay.

Please apply here: faculty-emory.icims.com/jobs/156609/...
#BYourCell

You will identify immune mechanisms underlying neuroinflammatory autoimmune diseases in children vs adults--autoimmune encephalitis (AE) and multiple sclerosis (MS). You will apply multi-omics single-cell assays to study the role of B/plasma cells and antibodies using patient samples (CSF and blood)

We are #hiring a postdoctoral fellow! Please share!

Join our B-cell team @Emory to lead NIH-funded projects on human neuro-immunology and autoimmunity in a co-mentorship and collaboration with neuroscientist Dr. Grace Gombolay.

Please apply here: faculty-emory.icims.com/jobs/156609/...
#BYourCell

Main dish or Appetizer
1. Adas Polo & Lamb (Fatemeh Yousefian and Masoud Ilkhani)
2. Lasagna Bolognese (Alex Nazzari & Matt Stern)

Dessert
1. Mohanthal (Hardy Nariya)
2. Cesta di Frutta @katyely.bsky.social

No more #ELISA assays?

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572

Is it time to retire the ELISAs for #antibody measurements?
1/4

Cell Press: STAR Protocols STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

While #FlowLITE quantifies total antibodies, #FlowBEAT also quantifies antibody specificities to defined antigens. Both protocols can be adapted to your research needs.
4/4
star-protocols.cell.com/protocols/4572

Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19 Nasal IgA1 autoantibodies against IFN-α are associated with better COVID-19 prognosis, including fewer symptoms and robust anti–SARS-CoV-2 immunity.

For context: FlowLITE was based on our previous technology, FlowBEAT, which we developed to measure human antibody isotypes against #SARSCoV2 and autoantigens in the #nasal #mucosa of #COVID19 patients (Sci Transl Med, 2024)
3/4
www.science.org/doi/10.1126/...

#FlowLITE is a modular, easy-to-build, bead-based assay for characterizing & quantifying absolute number of antibodies in small volumes (~10μL) of any fluid specimen. This is Justin Hahm’s Honors Thesis project (Highest Honors!). He was mentored by PhD student Alex Nazzari, the co-first author.
2/4

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572

Is it time to retire the ELISAs for #antibody measurements?
1/4

Main dish or Appetizer
1. Adas Polo & Lamb (Fatemeh Yousefian and Masoud Ilkhani)
2. Lasagna Bolognese (Alex Nazzari & Matt Stern)

Dessert
1. Mohanthal (Hardy Nariya)
2. Cesta di Frutta @katyely.bsky.social

Our 🌈 Annual Cook-off event keeps growing! We build community around great food, drinks, and music. Thank you for the delicious dishes and fantastic company.

The 2025 Chef Laureate goes to...

It was great to present our lab's work "Engineering B-cell therapies for life-long immunity" at the Annual Research Day @emorydeptofmed.bsky.social

We discussed our Gates Foundation project and how studying human early-life B cells can inform this technology. Great discussions!
#25DOMResearchDay

It was great to present our lab's work "Engineering B-cell therapies for life-long immunity" at the Annual Research Day @emorydeptofmed.bsky.social

We discussed our Gates Foundation project and how studying human early-life B cells can inform this technology. Great discussions!
#25DOMResearchDay

What makes PhD students happy? Good supervision Supervisors who invest in positive mentoring relationships with their PhD candidates also reap the benefits for their own research.

What makes PhD students happy? Good supervision www.nature.com/articles/d41...

Establishing a Fixed Time Period of Admission and an Extension of Stay Procedure for Nonimmigrant Academic Students, Exchange Visitors, and Representatives of Foreign Information Media Unlike most nonimmigrant classifications, which are admitted for a fixed time period, aliens in the F (academic student), J (exchange visitor), and most I (representatives of foreign information media...

Comment period ends on Monday for a proposed rule change that would terminate student visas after 4 years. It also puts limits on exchange visitors and reps of foreign media, and shortens the length of time at the end of the visa from 60 days to 30 days.

www.federalregister.gov/documents/20...