The mass range of tandem mass spectra can be categorized into three main regions. For glycopeptides, the lower mass range contains mainly oxonium ions, the middle range contains mainly peptide fragments, and the high range contains charge-reduced fragments. All regions contain valuable fragment ion information, but they can only be simultaneously accessed by breaking the 5–10–15 rule.
MS/MS scan range can be an afterthought that doesn't receive attention in method design, but improper settings can affect experimental outcomes. This is especially true in glycoproteomics.
A #glycotime thread about a new #JASMS paper on this idea from @riley-research.bsky.social
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27.02.2026 19:40
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Profiling Glycoproteins Enriched by Multinanoparticle Protein Corona
Biofluids, such as plasma and cerebrospinal fluid (CSF), present significant challenges for proteome and glycoproteome analysis due to their complex protein composition and wide dynamic range of prote...
A month behind the times, but I wanted to share an update from @emmajays.bsky.social and other co-authors in @riley-research.bsky.social. Our manuscript "Profiling Glycoproteins Enriched by Multinanoparticle Protein Corona" is now published in Analytical Chemistry:
pubs.acs.org/doi/abs/10.1...
10.02.2026 15:59
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Awards for Undergraduate Students
Visit the post for more.
Do you know outstanding undergraduate researchers who could use up to $1,000 in travel funding to present their work at the Spring @acs.org or @pittcon.bsky.social Meetings?
Please encourage them to apply for a Kolthoff award! Deadline is Jan. 12, 2026.
acsanalytical.org/undergraduat...
25.11.2025 20:27
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US HUPO - Distinguished Contribution Award
Congratulations to Mike MacCoss on receiving the Donald F. Hunt Distinguished Contribution in Proteomics Award from US HUPO!
us-hupo.org/Distinguishe...
12.11.2025 17:12
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US HUPO - Computational Proteomics Award
Congratulations to Bill Noble on receiving the 2026 Gil Omenn Computational Proteomics Award from US HUPO!
us-hupo.org/Computationa...
12.11.2025 17:14
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Have fun with the poster presentation today @timveth.bsky.social!
11.11.2025 16:30
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@timveth.bsky.social is not only attending #SFG2025 - he helped organize Glycopalooza events for early career researchers and is presenting poster B10-110 Tuesday. Check it out in session 2 to hear his impressive work integrating bottom-up and top-down methods to define glycoproteoform heterogeneity
10.11.2025 21:15
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The overlap of #HUPO2025 with #SFG2025 this week means I’m missing out on the #glycotime fun at SFG in San Diego.
I am happy postdoc @timveth.bsky.social is able to represent @riley-research.bsky.social at SFG instead!
10.11.2025 21:15
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If you are at #HUPO2025 and want to see some recent work from @riley-research.bsky.social, come check out Kayla’s talk on Monday afternoon in the Glycoproteomics session in the Frontenac Ballroom!
My poster on our recent work informatics tools is on Monday afternoon too. See you there!
#glycotime
09.11.2025 22:34
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🧪#glycotime
16.10.2025 18:35
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Our manuscript describing @riley-research.bsky.social's GlyCounter informatics tool is now in press at Molecular and Cellular Proteomics. Congrats to first-author Katie Kothlow, her first first-author paper!
The manuscript is available here: www.mcponline.org/article/S153...
16.10.2025 18:05
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Awesome to see GlyCounter at work!
www.biorxiv.org/content/10.1...
27.09.2025 00:54
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Awesome to see GlyCounter at work!
www.biorxiv.org/content/10.1...
27.09.2025 00:54
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Can nanoparticles help us probe the glycoproteome?
The new pre-print from @riley-research.bsky.social seeks to answer this question using the Proteograph technology from Seer Inc.
Check out this lovely study on glycoproteins NP-enriched from biofluids here: chemrxiv.org/engage/chemr...
24.09.2025 07:04
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Have you wondered how nanoparticle protein coronas (e.g., Seer’s Proteograph XT) can help with glycoprotein enrichment in biofluids?
We were curious, too, and this new @riley-research.bsky.social pre-print led by outstanding postdoc @emmajays.bsky.social details our #glycotime investigations.
24.09.2025 13:35
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This was so much fun! It was great to present and meet other incredible undergraduates at #ASMS2025. A huge thank you to @riley-research.bsky.social group members for their unwavering encouragement and even bringing me food and water during the presentations!
02.06.2025 22:55
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#ASMS2025
Come here about the latest in real-time instrument control including applications to single cell proteomics (@parallelsq.bsky.social), #glycotime, and quantitative proteomics!
02.06.2025 12:40
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#ASMS2025 offers great evening workshops. If you are interested in real-time decision-making during MS data acquisition or still looking for one to join,
consider the Real time Mass Spectrometry Workshop featuring speakers and panelists Sarah Sipe, Aarthie Senathirajah, and Manuel Peris Diaz.
02.06.2025 11:55
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Great to see #proudofalumni @nmriley.bsky.social on the @cenmag.bsky.social Talented 12 list! 🎉 🏆 Check out his #glycotime research program:
www.riley-research.com
24.05.2025 05:26
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Who's that T12-er? Nick Riley: "I was asking a lot more questions about the instrument than I was about the experiments."
@nmriley.bsky.social of @riley-research.bsky.social is pursuing better ways to study the biochemistry of sugars that decorate cell surfaces. He hopes that someday scientists could analyze these sugars en masse to infer the health of the cell. Read more: cen.acs.org/biological-c...
#CENT12 #chemsky🧪
28.05.2025 11:12
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@riley-research.bsky.social is excited for #ASMS2025 in Baltimore!
We made sure to capitalize on some of the classics, like a crab dinner at LP Steamers and an Orioles game, before the conference festivities kicked off.
Come hear what we have been up to!
02.06.2025 02:53
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We are lucky to have multiple people to celebrate in @riley-research.bsky.social recently. This time graduate student Kayla Markuson won 2 (!) @uwchemistry.bsky.social awards:
- Outstanding Teaching Assistant Award
- Excellence in Graduate Leadership and Service Award
Way to go Kayla!
08.05.2025 18:03
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Truthfully, I am proud of all the @riley-research.bsky.social members who applied for the GRFP, both this year and last. These are highly competitive awards, and it is tough not to be selected, but the scientific and personal growth I see throughout the process is always impressive and inspiring.
10.04.2025 19:14
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Another proud PI post today 🎉 , this time celebrating one of our stellar undergraduate researchers, @vishnutejus.bsky.social.
Vishnu received the @asms.org Undergraduate Student Award to present his work on an updated spectral annotator at the annual conference in June.
Congrats Vishnu!
11.04.2025 15:00
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Congratulations to Chris McGann and Bo Wen on receiving graduate student awards at the 2025 ASMS Conference!
11.04.2025 16:27
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Time for a proud PI post 🎉 : Katie Kothlow from @riley-research.bsky.social received an Honorable Mention from the National Science Foundation (NSF) Graduate Research Fellowship Program! Congrats, Katie!
10.04.2025 19:14
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Congratulations @nmriley.bsky.social! #proudofalumni
08.04.2025 15:18
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The Biochemical Society 2026 Awards. Early Career Research Award: Dr Nicholas M. Riley of University of Washington, USA
A world-class researcher and scholar, we are thrilled to present Nicholas Riley with an Early Career Research Award in 2026! @nmriley.bsky.social's research group pursues an exciting vision that leverages new technology development to solve critical problems in glycoscience (and beyond!).
08.04.2025 11:15
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