Volkan K. Koseoglu

Proteoglycofili are glycosaminoglycan-containing fibrillar components released by neutrophils to neutralize bacteria Alternative to the formation of NETs, André et al. describe here that viable neutrophils may release a fibrillar component named proteoglycofili or PGF upon stimulation. PGF formation is associated wi...

Thrilled to share that neutrophils produce extra long fibrillar components named proteoglycofili or PGF. PGF are released by living neutrophils and do not contain DNA. They might look like NETs but are not NETs…

www.cell.com/cell-reports...

Activation of the Yersinia type III secretion system induces large-scale spatial reorganization of chromosomal and virulence plasmid DNA Activation of the Yersinia type III secretion system triggers rapid, large-scale relocalization of both chromosomal and plasmid DNA. This spatial reorganization links secretion activity to growth inhi...

Activation of the Yersinia type III secretion system induces large-scale spatial reorganization of chromosomal and virulence plasmid DNA: Cell Reports www.cell.com/cell-reports...

Excited to share our lab’s first preprint!

A huge shout-out to our lab tech, Jonah Lanier, for driving this project.

Enteropathogenic bacteria evade ROCK-driven epithelial cell extrusion - Nature The bacterial ubiquitin ligase NleL evades host defence mechanisms both by inhibiting pyroptosis and by preventing infected intestinal epithelial cells from being extruded into the lumen and expelled ...

Check out our new publication, a collaboration with Giovanni Luchetti and Vishva Dixit @ Genentech.
We show that the E.coli effector NleL inhibits intestinal epithelial cell extrusion after #inflammasome activation via degrading the kinases ROCK1 and 2!!
www.nature.com/articles/s41...

Explainer 1/8

Multilevel regulation of c-di-GMP biosynthesis by cAMP signaling increases Shigella sonnei fitness and pathogenicity in response to bile salts It was previously demonstrated that intestinal pathogens have evolved different mechanisms to enhance their infection and colonization in the intestin…

www.sciencedirect.com/science/arti...

Neutralizing antibodies induced by IpaB and IpaC mRNA vaccines inhibit Shigella flexneri invasion | Microbiology Spectrum This study aimed to utilize mRNA vaccine technology targeting conserved antigens (IpaB and IpaC) to overcome the limitations of previous Shigella vaccines. By demonstrating functional immune responses...

Neutralizing antibodies induced by IpaB and IpaC mRNA vaccines inhibit Shigella flexneri invasion | Microbiology Spectrum journals.asm.org/doi/full/10....

Macrophages orchestrate elimination of Shigella from the intestinal epithelial cell niche via TLR-induced IL-12 and IFN-γ Eislmayr et al. reveal a previously unrecognized role of macrophages in controlling the intracellular pathogen Shigella. By sensing bacteria and triggering IFN-γ production, macrophages, not neutrophi...

Macrophages orchestrate elimination of Shigella from the intestinal epithelial cell niche via TLR-induced IL-12 and IFN-γ: Cell Host & Microbe www.cell.com/cell-host-mi...

Targeting deubiquitinating enzymes and ubiquitin pathway modulators to enhance host defense against bacterial infections | mBio Antibiotic-resistant infections, particularly those caused by intracellular pathogens, represent an urgent public health threat due to their ability to evade immune responses and resist conventional a...

Targeting deubiquitinating enzymes and ubiquitin pathway modulators to enhance host defense against bacterial infections | mBio journals.asm.org/doi/10.1128/...

Role of listeriolysin O and phospholipases C in L. monocytogenes intercellular protrusion dynamics, resolution, and autophagy avoidance | mBio Intracellular bacterial pathogens, including L. monocytogenes, spread from cell to cell to effectively disseminate within host tissues and cause systemic infections. We developed a fluorescence micros...

Role of listeriolysin O and phospholipases C in L. monocytogenes intercellular protrusion dynamics, resolution, and autophagy avoidance | mBio journals.asm.org/doi/full/10....

Please share my 1 min video on the importance of maintaining NIH funding of infectious diseases and drug discovery research vs rising antibiotic resistance

Drastic budget cuts harm our health and imperil the next generation of scientists

More @UCSanDiego “Behind Every Breakthrough” bit.ly/3FlXQs3

Host glutathione is required for Rickettsia parkeri cell division and intracellular survival - Nature Communications Rickettsia species are bacterial pathogens that obligately reside in the host cell cytosol. Here, Sun et al. report that Rickettsia use host glutathione as a nutrient source that enables bacterial cel...

www.nature.com/articles/s41...

Only 328 days to go! We're very busy behind the scenes getting thigns ready for the meeting! In the meantime you can add your email address for updates here: coming-soon.embo.org/w26-04

1. Today the NIH director issued a new directive slashing overhead rates to 15%.

I want to provide some context on what that means and why it matters.

grants.nih.gov/grants/guide...

VopX, a novel Vibrio cholerae T3SS effector, modulates host actin dynamics | mBio Despite different infection strategies, enteric pathogens commonly employ a T3SS to colonize the human host and cause disease. Effector proteins are unique to each T3SS-encoding bacterial species and ...

journals.asm.org/doi/full/10....

Klebsiella pneumoniae employs a type VI secretion system to overcome microbiota-mediated colonization resistance - Nature Communications Through metagenomics, Bray et al. identified Klebsiella pneumoniae interacting microbiota partners in the gut, and a transposon mutagenesis screen revealed a role for its T6SS in gut colonization, wit...

www.nature.com/articles/s41...

ERK activation waves coordinate mechanical cell competition leading to collective elimination via extrusion of bacterially infected cells Epithelial cells respond to infection with the intracellular bacterial pathogen Listeria monocytogenes by altering their mechanics to promote collecti…

www.sciencedirect.com/science/arti...

Bacteria use exogenous peptidoglycan as a danger signal to trigger biofilm formation - Nature Microbiology Peptidoglycan released by neighbouring kin or non-kin cell lysis induces physiological changes that protect from a range of stresses, including phage predation.

www.nature.com/articles/s41...

Characterization of Shigella flexneri serotype 6 strains from geographically diverse low- and middle-income countries | mBio Shigellosis is an ongoing global public health crisis with >270 million annual episodes among all age groups; however, the greatest disease burden is among children in low- and middle-income countr...

journals.asm.org/doi/full/10....

A temporary cholesterol-rich diet and bacterial extracellular matrix factors favor Salmonella spp. biofilm formation in the cecum | mBio Typhoid fever is a systemic infectious disease triggered by the gastrointestinal dissemination of Salmonella Typhi and Paratyphi in humans. Three to five percent of infected individuals become chronic...

journals.asm.org/doi/full/10....

Excited to share our latest, "Biofilm dispersal patterns revealed using far-red fluorogenic probes." @plos.bsky.social We developed a cell-labeling strategy using far-red dyes to image dense microbial communities (where fluorescent proteins often do not work well) journals.plos.org/plosbiology/...

Could you please add me too?

A conserved interaction between the effector Sca4 and host clathrin suggests additional contributions for Sca4 during rickettsial infection | Infection and Immunity Intracellular bacterial pathogens must adapt to a host cell environment to enable their growth, survival, and dissemination. Consequently, they have evolved various mechanisms to manipulate their host environments to create a more hospitable niche (1–5). One common way for pathogens to interact with host cells is through surface-associated and secreted effector proteins. Bacterial pathogen effectors are known to exert diverse functions, including inducing cellular invasion, establishing vacuolar compartments, remodeling host organelles, modifying the host immune response, and facilitating the spread of these pathogens between cells (6–11).

journals.asm.org/doi/abs/10.1...

#Shigella and #ETEC main causes of #pediatric #diarrhea in Rwanda after 10 years of #Rotavirus vaccine.
Highlights vaccine success but continued burden of #enteric bacterial pathogens
🦠 🧫 #MicroSky
academic.oup.com/jid/article/...

I'd like to be added!

SPATEs promote the survival of Shigella to the plasma complement system upon local hemorrhage and bacteremia

www.pnas.org/doi/10.1073/...

Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation | mBio Inflammasomes are essential for host defense against intracellular bacterial pathogens like Legionella, as they activate caspases, which promote cytokine release and cell death to control infection. I...

From the S. Shin lab sunnyshinlab.bsky.social: Hu guanylate-binding protein 1 (GBP1) damages the vesicle around Legionella, revealing the pathogen to the cell by activating inflammasomes. In contrast, murine GBPs can directly attack cytosolic Legionella. doi.org/10.1128/mbio... #mbio #ASM #MicroSky

Can colleagues amplify this appeal to journals and journal editors to open accounts on Bluesky, please? @MicrobioSoc @TrendsMicrobiol @CellCellPress @JBacteriology @jbiolchem @Nature @ScienceMagazine @PNASNews @MolMicroEditors @NAR_Open @NatureMicrobiol @NatureComms @ASMicrobiology 🙏
🦠🧪🧫🧬

Making contact away from home: a bacterial secreted effector mediates inter-organelle communication | EMBO reports EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.

www.embopress.org/doi/full/10....

MAMPM February 2-4, 2025 Wintergreen Resort, Virginia

Calling all microbiologists! Registration is open for the Mid-Atlantic Microbial Pathogenesis Meeting, February 2-4, 2025.! Abstract deadline is 12/8/2024, but don't wait, filling fast. Check it out at mampm.org.