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Richard Held

@rgsheld

Neuroscientist / synaptic physiologist interested in protein topography and cellular signal transduction. Ephys in the past, cryoET now, mouse genetics forever. https://scholar.google.com/citations?hl=en&user=ESpBK1AAAAAJ

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28.07.2023
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Latest posts by Richard Held @rgsheld

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More field trips is my wish

07.01.2026 05:14 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

I just went to the gym...but it's Jan. 1 so PR for the year

02.01.2026 03:30 πŸ‘ 0 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

Set my deadlift PR for the year 🀫

02.01.2026 02:26 πŸ‘ 4 πŸ” 0 πŸ’¬ 2 πŸ“Œ 0
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Holiday triptych

25.12.2025 21:06 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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June does christmas right

25.12.2025 03:51 πŸ‘ 3 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
08.12.2025 02:13 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

Holds up:

www.mcsweeneys.net/articles/the...

07.12.2025 23:32 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
Implementing a Unified NIH Funding Strategy to Guide Consistent and Clearer Award Decisions | Grants & Funding

Image is from:

grants.nih.gov/news-events/...

Sources for the idiocy are harder to narrow down...

07.12.2025 06:09 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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Absolutely insane paragraph

07.12.2025 02:49 πŸ‘ 2 πŸ” 1 πŸ’¬ 0 πŸ“Œ 2

The Nobel Prize committee should announce the World Cup winner tomorrow

06.12.2025 04:29 πŸ‘ 38711 πŸ” 7481 πŸ’¬ 508 πŸ“Œ 300
PNAS Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers!

Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...

24.11.2025 20:54 πŸ‘ 16 πŸ” 7 πŸ’¬ 1 πŸ“Œ 1

I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell.

authors.elsevier.com/a/1mA6h,LqAr...

01.12.2025 15:43 πŸ‘ 15 πŸ” 4 πŸ’¬ 0 πŸ“Œ 0

ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/41315814/ #cryoEM

30.11.2025 23:30 πŸ‘ 1 πŸ” 2 πŸ’¬ 0 πŸ“Œ 0

Thanks! The center ones didnt get quite the color I wanted but I'm happy with it.

28.11.2025 03:11 πŸ‘ 1 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Not bad, not great

28.11.2025 02:16 πŸ‘ 6 πŸ” 0 πŸ’¬ 2 πŸ“Œ 0
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Soon....

28.11.2025 00:25 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 1
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So it begins

27.11.2025 02:30 πŸ‘ 3 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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Clearly a poorly resolved flexible domain here

18.11.2025 00:40 πŸ‘ 3 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
Timing of neurotransmission at fast synapses in the mammalian brain - Nature Nature - Timing of neurotransmission at fast synapses in the mammalian brain

Important to remember how fast the fusion process actually is! Millisecond experimental temporal resolution is hard to achieve but synchronous fusion is happening within hundreds of microseconds of action potential onset.

www.nature.com/articles/384...

07.11.2025 21:50 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

I'm not entirely on board with the proposed temporal sequence but I think the size of the dataset convincingly demonstrates the presence of "small" and "large" membrane proximal vesicle populations. A more tempting model imo would be that these small vesicles preferentially fuse asynchronously.

07.11.2025 21:50 πŸ‘ 0 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0
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β€œKiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...

Finally got to go through this carefully. An interesting paper and a tremendous amount of work.

www.science.org/doi/10.1126/...

07.11.2025 21:50 πŸ‘ 6 πŸ” 1 πŸ’¬ 1 πŸ“Œ 0

Gonna need somebody to explain daylight savings to my dogs

03.11.2025 02:11 πŸ‘ 2 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

www.theatlantic.com/ideas/archiv...

02.11.2025 02:28 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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02.11.2025 02:28 πŸ‘ 3 πŸ” 1 πŸ’¬ 1 πŸ“Œ 0
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I think about this tweet on a near-daily basis

09.10.2025 16:11 πŸ‘ 3 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0
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This is what made America great

08.10.2025 16:41 πŸ‘ 4 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0

Booooo

07.10.2025 15:37 πŸ‘ 0 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

The cat is officially out of the bag!

The LMB is home to a unique community of outstanding connectomics scientists β€” I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!

This is going to be the most exciting journey of my scientific career β€” more soon! 🌈🧠

15.09.2025 09:54 πŸ‘ 34 πŸ” 5 πŸ’¬ 5 πŸ“Œ 0
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Heart rate data or RC voltage step?

08.08.2025 15:14 πŸ‘ 1 πŸ” 0 πŸ’¬ 1 πŸ“Œ 0

Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?

17.07.2025 16:18 πŸ‘ 0 πŸ” 0 πŸ’¬ 0 πŸ“Œ 0