More field trips is my wish
@rgsheld
Neuroscientist / synaptic physiologist interested in protein topography and cellular signal transduction. Ephys in the past, cryoET now, mouse genetics forever. https://scholar.google.com/citations?hl=en&user=ESpBK1AAAAAJ
More field trips is my wish
I just went to the gym...but it's Jan. 1 so PR for the year
Set my deadlift PR for the year π€«
Holiday triptych
June does christmas right
Holds up:
www.mcsweeneys.net/articles/the...
Image is from:
grants.nih.gov/news-events/...
Sources for the idiocy are harder to narrow down...
Absolutely insane paragraph
The Nobel Prize committee should announce the World Cup winner tomorrow
Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers!
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell.
authors.elsevier.com/a/1mA6h,LqAr...
ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms pubmed.ncbi.nlm.nih.gov/41315814/ #cryoEM
Thanks! The center ones didnt get quite the color I wanted but I'm happy with it.
Not bad, not great
Soon....
So it begins
Clearly a poorly resolved flexible domain here
Important to remember how fast the fusion process actually is! Millisecond experimental temporal resolution is hard to achieve but synchronous fusion is happening within hundreds of microseconds of action potential onset.
www.nature.com/articles/384...
I'm not entirely on board with the proposed temporal sequence but I think the size of the dataset convincingly demonstrates the presence of "small" and "large" membrane proximal vesicle populations. A more tempting model imo would be that these small vesicles preferentially fuse asynchronously.
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
Gonna need somebody to explain daylight savings to my dogs
www.theatlantic.com/ideas/archiv...
I think about this tweet on a near-daily basis
This is what made America great
Booooo
The cat is officially out of the bag!
The LMB is home to a unique community of outstanding connectomics scientists β I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career β more soon! ππ§
Heart rate data or RC voltage step?
Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?